135 research outputs found
The Molecular Cloning and Expression of Human Placental Matrix and Surface Proteins
Full text linkFibronectin protein isoforms have several functions in the body and play a vital role in maintaining the integrity of human placenta. The multifunctional properties of FN may have been mediated by the ability of FN isoforms to bind to cells and other extracellular matrix components such as collagens and fibrin. These and other functional properties which have been assigned to FN are thought to have been made possible by the existence of multiple FN isoforms
A COMMON FIXED POINT FOR WEAK φ-CONTRACTIONS ON <i>b</i>-METRIC SPACES
No full textaydi, hassen/0000-0003-4606-7211; Moradi, Sirous/0000-0002-8640-7252; , Hassen/0000-0003-3896-3809In this paper, we give a common fixed point result, for single-valued and multi-valued mappings satisfying a weak phi-contraction in b-metric spaces. Presented theorems extend, generalize and improve some existing results in the literature. Some examples are also given.Romanian National Authority for Scientific Research, CNCS UEFISCDI [PN-II-ID-PCE-2011-3-0094]The second author is partially supported by a grant of the Romanian National Authority for Scientific Research, CNCS UEFISCDI, project number PN-II-ID-PCE-2011-3-0094.Science Citation Index Expande
Prenatal diagnosis of citrullinemia type 1; seven families with c.1168G>A mutation of Argininosuccinate synthetase 1 gene in Southwest Iran: A case series
Full text linkBackground: Citrullinemia type 1 is an autosomal recessive disease resulting in ammonia accumulation in the blood, and if uncontrolled may progress to coma or death in the early months after birth.
Cases presentation: 7 families from Southwest Iran having one or more children in their families or relatives, who died in the early months after birth due to citrullinemia type 1 visited for genetic counseling and prenatal diagnosis. Whole-exome sequencing was performed on peripheral blood specimens and chorionic villus samples. Sanger sequencing confirmed the genetic results. Both parents were identified as carriers for the exon 15 c.1168G>A mutation in each family. The fetus in 6 out of 7 families was homozygote for A substitution on the argininosuccinate synthetase 1 gene.
Conclusion: The presence of a common mutation in the argininosuccinate synthetase 1 gene in all affected families of Southwest Iran shows a possible population cluster in this area.
Key words: Argininosuccinate synthetase, Chorionic villus sampling, Point mutation
Microsatellite analysis of mouse TNF and Cypla2 loci for polymorphism: detection and evaluation of genetic contamination
Full text linkThe mouse TNF locus comprises tandemly arranged genes coding for Tnf _ (cachectin) and Tnf ß (lymphotoxin). The mouse TNF and Cypla2 loci were selected to detect simple sequence length polymorphism on genomic DNAs of four laboratory mouse strains. These polymorphisms might be useful in the search for tumor supressor genes involved in specific cancers. The primary aim of this work was to analyse the use of these polymophic microsatellite markers, which could differentiate between four typical laboratory mouse strains (i.e. BALB/c, C57BL/6, CBA as three inbred mouse strains and Syrian Hamster as an outbred strain) and the second was to define possible genetic contamination of a BALB/c mouse colony. In this study, we investigated the applicability of DNA fingerprinting using short tandem repeats (STRs) by the use of PAGE and silver staining. We found that all microsatellite DNA sequences varied in size in the TNF locus in the four strains tested, but for the Cypla2 locus no polymorphism was found within these strains. To our knowledge this is the first report of lack of polymorphism in the Cypla2 locus within these strains. The other techniques for inbred mouse identification were skin grafting and a coat colour test. We found no signs of rejection during skin grafting within the BALB/c mouse strain and also as expected all strains showed normal phenotypes. These experiments suggested lack of genetic heterogeneity in Cypla2 locus and no evidence of genetic contamination was also found in the BALB/c mouse
Aberrant promoter hypermethylation of miR-335 and miR-145 is involved in breast cancer PD-L1 overexpression
No full textPD-L1 is one of the most important immune checkpoint molecules in breast cancer that plays an important role in suppressing the immune system when confronted with tumor cells and is regulated by various microRNAs. Among them, microRNA-335-3p and microRNA-145-5p, regulated by DNA methylation, have tumor suppressor activities. We studied the role of miR-335 and -145 on PD-L1 suppression in breast cancer. The expression of miR-355 and miR-145 was significantly downregulated in BC tissues and cell lines compared to their controls, and their downregulation was negatively correlated with PD‐L1 overexpression. In-silico and luciferase reporter systems confirmed that miR-335 and -145 target PD-L1. In BC tissues and cell lines, cancer-specific methylation was found in CpG-rich areas upstream of miR-335 and-145, and up-regulation of PD-L1 expression was connected with hypermethylation (r = 0.4089, P = 0.0147, and r = 0.3373, P = 0.0475, respectively). The higher levels of miR-355 and -145 in BC cells induced apoptosis, arrested the cell cycle, and reduced proliferation significantly. In summary, we found that miR-335 and -145 are novel tumor suppressors inactivated in BC, and these miRs may serve as potential therapeutic targets for breast cancer treatment
Genetic modification of mesenchymal stem cells to overexpress CXCR4 and CXCR7 does not improve the homing and therapeutic potentials of these cells in experimental acute kidney injury
No full textThe therapeutic potential of bone marrow mesenchymal stem cells (MSCs) in kidney failure has been examined in some studies. However, recent findings indicate that after transplantation, these cells home to kidneys at very low levels. Interaction of stromal derived factor-1 (SDF-1) with its receptor, CXCR4, is of pivotal importance in migration and homing. Recently, CXCR7 has also been recognized as another SDF-1 receptor that interacts with CXCR4 and modulates its functions. In this study, CXCR4 and CXCR7 were separately and simultaneously overexpressed in BALB/c bone marrow MSCs by using a lentiviral vector system and the homing and renoprotective potentials of these cells were evaluated in a mouse model of cisplatin-induced acute kidney injury. Using flow cytometry, immunohistochemistry, and real-time PCR methods for detection of GFP-labeled MSCs, we found that although considerably entrapped in lungs, native MSCs home very rarely to kidneys and bone marrow and this rate cannot be significantly affected by CXCR4 and/or CXCR7 upregulation. Transplantation of neither native nor genetically engineered MSCs ameliorated kidney failure. We concluded that overexpression of CXCR4 and CXCR7 receptors in murine MSCs cannot improve the homing and therapeutic potentials of these cells and it can be due to severe chromosomal abnormalities that these cells bear during ex vivo expansion
High level of intrafamilial phenotypic variability of non-syndromic hearing loss in a Lur family due to delE120 mutation in GJB2 gene
No full textRapid characterization of the CHO platform cell line and identification of pseudo attP sites for PhiC31 integrase
No full textCo-inheritance of αααanti 3.7 triplication with β-thalassemia trait in an Iranian family
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