100,321 research outputs found
Optimization of the “Nichoid” substrate for the Culture and Quantification of Stem Cell Function
No full text3D nichoid substrates affect mesenchymal stem cell morphology and euchromatin organization
No full textIn recent years, it has been demonstrated that the extracellular microenvironment plays a pivotal role in controlling mesenchymal stem cell fate and functionality. At the basis, there is the mechanotransduction process by which cells sense physical forces and translate them into biochemical/biological responses. In order to modulate gene expression, cell mechanotransduction involves cell-substrate interaction and cytoskeleton proteins, but also nuclear geometry and chromatin remodelling. We developed an innovative isotropic 3D culture substrate, nanoengineered via two-photon laser polymerization, able to control nuclear shape. In this work, we studied cell cytoskeleton and chromatin organization of rat bone marrow mesenchymal stem cells seeded into our 3D scaffold and compared to cells grown on standard flat glass substrates. Our results show that the 3D micro-lattice structure inhibits the formation of actin cup and fibers joining cell periphery to the nucleus. It affects nuclear morphology and euchromatin organization. Interesting, similar results were obtained on cells treated with a drug able to inhibit actin polymerization and cytoskeleton organization. In order to correlate euchromatin localization and organization with cell fate and functionality, future work will be focalized investigating gene expression of MSC cultured on standard glass 2D substrate and into our 3D micro-lattice structure
Multi-foci laser microfabrication of 3D polymeric scaffolds for stem cell expansion in regenerative medicine
No full textCharacterization of Stem Cell Differentiation in a 3D Nichoid Scaffold Through Label-free Multimodal Microscopy
No full textNuclear internalization kinetics of a permeable fluorescent dye in cell nuclei of different shape
No full text3d stem cell niche engineering via two-photon laser polymerization
No full textA strategy to modulate the behavior of stem cells in culture is to mimic structural aspects of the native cellâextracellular matrix (ECM) interaction. An important example of such artificial microenvironments for stem cell culture is the so-called âsynthetic niche.â Synthetic niches can be defined as polymeric culture systems mimicking at least one aspect of the interactions between stem cells and the extracellular surroundings, including biochemical factors (e.g., the delivery of soluble factors) and/or biophysical factors (e.g., the microarchitecture of the ECM). Most of the currently available approaches for scaffold fabrication, based on self-assembly methods, do not allow for a submicrometer control of the geometrical structure of the substrate, which might play a crucial role in stem cell fate determination. A novel technology that overcomes these limitations is laser two-photon polymerization (2PP). Femtosecond laser 2PP is a mask-less direct laser writing technique that allows manufacturing three dimensional arbitrary microarchitectures using photosensitive materials. Here, we report on the development of an innovative culture substrate, called the ânichoid,â microfabricated in a hybrid organicâinorganic photoresist called SZ2080, to study mesenchymal stem cell mechanobiology
Letter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Implantable medical device
No full textAn implantable medical device obtained by means of two- photon laser polymerisation of a resin to form a three-dimensional matrix, wherein: said three-dimensional matrix comprises a number of levels distributed in height; and said three-dimensional matrix comprises reference means designed to uniquely identify the height of each level from a pre-set reference, by means of a multiphoton fluorescence-excitation microscope; said implantable medical device being characterised in that said reference means comprise a solid having a cross section that varies with height
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