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Association study between SNPs in ACACA, ACLY, FASN, and SCD genes and fatty acid composition of backfat and muscle in Italian Large White pigs
No full textIsolation and mapping of two porcine skeletal muscle myosin heavy chain isoforms
No full textThe present paper describes the isolation and linkage mapping of two isoforms of skeletal muscle myosin heavy chain in pig. Two partial cDNAs (pAZMY4 and pAZMY7), coding for the porcine myosin heavy chain-2B and -β respectively, have been isolated from a pig skeletal muscle cDNA library. Four RFLPs were detected with the putative porcine skeletal myosin heavy chain-2B probe (pAZMY4) and one RFLP was identified with the putative myosin heavy chain-β probe (pAZMY7). Two myosin heavy chain loci were mapped by linkage analysis performed with the five RFLPs against the PiGMaP linkage consortium ResPig database: the MYH1 locus, which identifies the fast skeletal muscle myosin heavy chain gene cluster, was located at the end of the map of porcine chromosome 12, while the MYH7 locus, which identifies the myosin heavy chain-α/-β gene cluster, was assigned to the long arm of porcine chromosome 7
Muscle transcriptome analysis identifies genes involved in ciliogenesis and the molecular cascade associated with intramuscular fat content in Large White heavy pigs
Full text linkIntramuscular fat content (IMF) is a complex trait influencing the technological and sensorial features of meat products and determining pork quality. Thus, we aimed at analyzing through RNA-sequencing the Semimembranosus muscle transcriptome of Italian Large White pigs to study the gene networks associated with IMF deposition. Two groups of samples were used; each one was composed of six unrelated pigs with extreme and divergent IMF content (0.67 ± 0.09% in low IMF vs. 6.81 ± 1.17% in high IMF groups) that were chosen from 950 purebred individuals. Paired-end RNA sequences were aligned to Sus scrofa genome assembly 11.1 and gene counts were analyzed using WGCNA and DeSeq2 packages in R environment. Interestingly, among the 58 differentially expressed genes (DEGs), several were related to primary cilia organelles (such as Lebercilin 5 gene), in addition to the genes involved in the regulation of cell differentiation, in the control of RNA-processing, and G-protein and ERK signaling pathways. Together with cilia-related genes, we also found in high IMF pigs an over-expression of the Fibroblast Growth Factor 2 (FGF2) gene, which in other animal species was found to be a regulator of ciliogenesis. Four WGCNA gene modules resulted significantly associated with IMF deposition: grey60 (P = 0.003), darkturquoise (P = 0.022), skyblue1 (P = 0.022), and lavenderblush3 (P = 0.030). The genes in the significant modules confirmed the results obtained for the DEGs, and the analysis with "cyto- Hubba" indicated genes controlling RNA splicing and cell differentiation as hub genes. Among the complex molecular processes affecting muscle fat depots, genes involved in primary cilia may have an important role, and the transcriptional reprogramming observed in high IMF pigs may be related to an FGF-related molecular cascade and to ciliogenesis, which in the literature have been associated with fibro-adipogenic precursor differentiation
Identification of differentially expressed small RNAs and prediction of target genes in Italian Large White pigs with divergent backfat deposition.
No full textA BG/II POLYMORPHISM AT THE PORCINE TRANSFERRIN (TF) LOCUS
No full textA BGL II POLYMORPHISM AT THE PORCINE TRANSFERRIN (TF) LOCU
A HincII polymorphism at the porcine gamma-glutamyl transpeptidase (GGT) locus
No full textA HincII polymorphism at the porcine gamma-glutamyl transpeptidase (GGT) locu
HINCII AND PSTI REVEAL POLYMORPHISMS AT THE PORCINE OROSOMUCOID (ORM) LOCUS (VOL 25, PG 369, 1994)
No full textHINCII AND PSTI REVEAL POLYMORPHISMS AT THE PORCINE OROSOMUCOID (ORM) LOCU
A BgIII RFLP at the porcine calpastatin (CAST) locus
No full textA BgIII RFLP at the porcine calpastatin (CAST) locu
A MspI polymorphism at the porcine γ‐glutamyl transpeptidase (GGT) locus
No full textA MspI polymorphism at the porcine γ‐glutamyl transpeptidase (GGT) locu
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