177,181 research outputs found
FISH testing of HER2 IHC 1+ early breast cancer with unfavorable prognostic factors
No full textF. Giotta2, S. Petroni 1, L. Caldarola 1, A. Latorre 2, R. Mallamaci 3, V. Lorusso 2, M. Zambetti 1, G. Simone 1
1 Pathology Department, NCRC Istituto Tumori “Giovanni Paolo II”, Bari, Italy
2 Medical Oncology Unit, NCRC Istituto Tumori “Giovanni Paolo II”, Bari, Italy
3 Human Physiology Institute, University of Bari, Italy
FISH testing of HER2 IHC 1+ early breast cancer with unfavorable prognostic factors
Background HER2-positive tumors are associated with a poor prognosis and a shortened disease-free and overall survival as well as with other unfavorable prognostic tumor characteristics (high histological grade, high proliferative index, negative or low estrogen receptor expression, etc.). HER2-positive tumors are also responsive to treatment with trastuzumab in reducing the risk of recurrence and improving survival. The aim of this study is to assess the incidence of HER2 gene amplification in selected tumors with adverse prognostic features which scored 1+ by immunohistochemistry (IHC).
Methods 75 women with infiltrating ductal carcinoma (IDC) and infiltrating lobular carcinoma (ILC) scoring 1+ by IHC were included. Forty-eight invasive breast carcinoma samples were selected according to unfavorable prognostic tumor characteristics and tested by FISH. HER2 amplification was evaluated using Vysis HER2/Cep17 probe (Path Vysion HER2 DNA Probe Kit®, Abbott Molecular, IL); ratio–based amplification was considered present when the HER2/Cep17 ratio was 2 or more and copy number-based amplification was considered present when the mean HER2 copy number was more than 6, in agreement with the ASCO/CAP/SIAPEC guidelines.
Results In 2013, 331 patients with invasive breast tumors were tested by IHC; 75 cases (23%) were scored 1+ of which 62 cases (19%) of IDC and 13 cases (4%) of ILC. Forty-eight invasive breast carcinoma samples (64%) were selected according to one or more unfavorable prognostic tumor characteristics; 22 out of 48 tumors (46%) showed high histological grade (G3); 27 cases (56%) had high proliferative index (Ki-67≥30%); 32 tumor samples (67%) were node-positive; and 29 cases (60%) showed vascular invasion. FISH was performed on 28 of the 1+ patients with adverse tumor characteristics and 6 IDC out of 48 (12.5%) showed HER2 amplification.
Conclusions Our preliminary retrospective data suggest that 6 patients out of 48 (12.5%) scoring 1+ by IHC show HER2 amplification, in agreement with the most recently published literature data. In order to not deny the benefit deriving from trastuzumab administration, in breast cancer patients showing IHC 1+, it is advisable to test HER2 gene amplification by FISH
Micrometeorological Measurement of ET. Part B: The role of canopy in modifying the partitioning of fluxes
No full textRadiofrequency Volumetric Reduction (ReVoR) for non-allergic and non-infectious perennial rhinitis.
No full textL’iperreattivita’ nasale puo’ essere considerata una causa favorente le flogosi recidivanti adenoidee?
No full textGastroesophageal reflux and nonspecific hyperreactivity of upper aerodigestive tract: possible correlations with otorhinolaryngological disorders.
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Understanding Data-Driven Product Service System characteristics: a conceptual framework for manufacturing applications
No full textThe explosion of digital technologies and data analytics capabilities are leading companies to rethink they offers and expand more than ever their business into the service domain. Specifically, considering manufacturers, the emerging possibility to connect products and create IoT architectures at the customer side, enable them to retrieve data flows during the products’ lifecycle. Analyze those data opened opportunities to obtain information to use intra-organization and at the same time to enhance already existing service or to develop new ones. Even the potential of data availability in this context is recognized in the literature, further work is still needed, especially defining how these new data-driven offers should be engineered and structured. In this view, this study provides a comprehensive interpretation on the general key components and characteristics of those services, defined as Data-Driven Product Service Systems (DDPSS), aiming at supporting the comprehension of specific principles and consequently the systematic creation of DDPSS. Indeed, limited research is devoted to the definition of the unique characteristics of those services and common agreement is still missing. The paper developed a two-hierarchical conceptual framework describing DDPSS typologies is proposed. The framework value is twofold: first it categories and harmonizes service typologies into a structured model and second, it can be used as a support tool during the service design phase, since it can inspire and guide service development. In the end, the paper also presents an explorative application of the conceptual framework within a different manufacturing company. The applications show both the descriptive and the prescriptive nature of the model; indeed, it is used to analyses the current position and to propose new trajectories for the companies’ service offering
Assessment of Cottle's areas through the application of a mathematical model deriving from acoustic rhinometry and rhinomanometric data.
No full textOBJECTIVES: Each nasal area, as defined by Cottle, has a different influence on the nasal airflow. The longitudinal distribution of resistances in nasal cavities was calculated by the anterior rhinomanometry and acoustic rhinometry data. DESIGN: Dynamic study of Cottle's areas in normal subjects was carried out by rhinomanometry and acoustic rhinometry. SETTING: Study by the Department of Otolaryngology of the University of Rome-La Sapienza. PARTICIPANTS: Twenty-seven Caucasian adults in local and general healthy conditions took part and completed this study, with a total of 54 nasal cavities included because of negativity at ENT-examination and clinical history, with normal respiratory parameters at the rhinomanometry and acoustic rhinometry. MAIN OUTCOME MEASURES: We determined nasal and acoustic resistances, nasal volumes and cross-sectional surface areas, as defined by Cottle, using nasal endoscopy. The longitudinal distribution of nasal resistances was obtained by integrating experimental surface areas using a novel mathematical model. The estimation of the longitudinal nasal resistance variations as a result of a theoretical reduction of the surface areas. RESULTS: The reduction of the 2-3-1 areas (in this order of importance) showed the greatest influence on the nasal resistances with coefficients of determinations greater than 0.98, this being quite different from that of the areas 4 and 5 for quite smaller area reduction percentages. CONCLUSIONS: The areas 2-3-1 control the overall nasal resistance so the surgical procedures on these areas greatly influence the dynamics of nasal airflow. The mathematical model developed here gives useful information to nasal functional surgery and may be applied to other schemes of nasal cavity
Non-specific nasal provocation test with histamine. Analysis of the dose-response curve.
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