1,744,492 research outputs found
Expression of ZAKI-4 in Mammalian Cells
No full text2003-12ZAKI-4 is a member of a novel gene family that functions as an endogenous calcineurin inhibitor. Two ZAKI-4 isoforms, α and β, display tissue-specific distribution and different hormonal regulation. However, functional differences of each ZAKI-4 isoforms have not been elucidated. Here, we show the subcellular localization of ZAKI-4 isoforms. Both ZAKI-4α and β showed the cytoplasmic distribution. These results suggest that ZAKI-4 seems to regulate calcineurin activity in the cytoplasm.国立情報学研究所で電子化したコンテンツを使用している。departmental bulletin pape
Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression
No full text2002-12ZAKI-4 inhibits the activity of calcineurin, a Ca^-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice.国立情報学研究所で電子化したコンテンツを使用している。departmental bulletin pape
Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone
No full text2002-12We identified ZAKI-4 as a thyroid hormone (T3) responsive gene in human skin fibroblasts. Our recent study revealed that two ZAKI-4 isoforms, α and β, are generated from a single gene on chromosome 6. Only expression of α is increased by T3, while that of β is not affected. Both isoforms belong to a family of proteins containing a conserved motif, which interacts with calcineurin and inhibits its activity. Recently, expression of a mouse homologue of the ZAKI-4 family gene (MCIP1) is shown to be induced by the activation of calcineurin in myocytes. We thus studied whether ZAKI-4 expression is also regulated by calcineurin-mediated pathway. Northern blot analysis revealed activation of calcineurin by ionomycin/phorbol 12-myristate 13-acetate (PMA) did not increase the expression of both isoforms. In addition, either basal expression of the two isoforms or T3-induced ZAKI-4α expression was not affected by FK506, a specific calcineurin inhibitor, suggesting the activation of calcineurin is not involved in ZAKI-4 regulation. However, cyclosporin A (CsA), another inhibitor of calcineurin decreased the basal expression of ZAKI-4α. Furthermore, T3-mediated increase of ZAKI-4α was completely blocked by CsA, but not by FK506. It is thus indicated that the two isoforms are differentially regulated, and the regulation of ZAKI-4α involves a CsA-sensitive pathway, which is independent of calcineurin activation.国立情報学研究所で電子化したコンテンツを使用している。departmental bulletin pape
Postfazione. Utopia e speranza: Bologna per Patrick Zaki
No full textThe essay elaborates on the talk the author delivered during the ceremony that took place after the liberation from prison of the UNIBO and honorary citizen of Bologna, Patrick Zaki. The essay elaborates on the following issues: the support of the university and city, the value of human rights, academic freedom, and public squares as spaces of democracy. It includes a longer part on utopia (as a literary genre and a political instrument of change) and hope, quoting academic scholars (Karl Mannheim, Ernst Bloch, Ursula Le Guin, Antonio Gramsci, Howard Zinn) and it explains the function of hope in utopia
An investigation on the mechanics of homogeneous expansion in gas-fluidized beds
No full textThe Richardson and Zaki (1954, Sedimentation and fluidization. Trans. Inst. Chem. Eng. 32, pp. 35–53.) equation has been used extensively to investigate the expansion profiles of homogeneous gas-fluidized beds. The experimental value of the parameter n appearing in the equation indicates how significantly interparticle forces affect the expansion of these beds, revealing the relative importance of these forces with respect to the fluid dynamic ones. In this work, we modeled the stable expansion of gas-fluidized beds of different diameter, accounting for enduring contacts among particles and wall effects. We solved the model numerically to obtain the bed expansion profiles, back-calculating from them the values of the parameter n. For all the cases considered, we observed that the values of n are higher than those obtained by purely fluid dynamic correlations, such as those advanced by Richardson and Zaki, and Rowe (1987, A convenient empirical equation for estimation of the Richardson and Zaki exponent. Chem. Eng. Sci. 42, pp. 2795.). This effect was more pronounced in beds of smaller diameter. To validate our model, we carried out fluidization and defluidization experiments, analyzing the results by means of the Richardson and Zaki equation. We obtained a reasonable agreement between numerical and experimental findings; this suggests that enduring contacts among particles, which are manifestations of cohesiveness, affect homogeneous bed expansion. This effect is amplified by wall friction
Genomic bins from "Genetic information exchange in microbial consortia determines the ability to biodegrade ibuprofen"
No full text<p>This record contains all the bins and MAGs binned from the wastewater-related microbial consortia described in the following article:</p>
<p>Zaki Saati-Santamaría, Pilar Navarro-Gómez, Juan A. Martínez-Mancebo, Maitane Juárez-Mugarza, Amando Flores, Inés Canosa. Genetic information exchange in microbial consortia determines the ability to biodegrade ibuprofen. <em>Under review</em>.</p>
<p>The gneomic features of each bin can be found in the supplementary material of the article.</p>
Beliefs about Self-Compassion (Chwyl, Chen, & Zaki, 2020)
No full textData and Code for “Beliefs about Self-Compassion: Implications for Coping and Self-Improvement”. Authors: Chwyl*, Chen*, and Zaki (2020
Data Archive For: Zaki et al., (2025), Large drainage systems produced half of Mars' ancient river sediment
No full textThe archive includes polygon shapefiles for 16 drainage systems. It also includes spreadsheets showing river profiles for two drainage systems (see Figure 4).
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Improvement of the Richardson-Zaki liquid-solid fluidisation model on the basis of hydraulics
No full textOne of the most popular and frequently used models for describing homogeneous liquid-solid fluidised suspensions is the model developed by Richardson & Zaki in 1954. The superficial fluid velocity and terminal settling velocity together with an index makes it possible to determine the fluid porosity in a straightforward way. The reference point for the Richardson-Zaki model is the terminal settling velocity at maximum porosity conditions. To be able to predict porosity in the proximity of minimum fluidisation conditions, either the minimum fluidisation velocity must be known or the Richardson-Zaki index must be very accurate. To maintain optimal process and control conditions in multiphase drinking water treatment processes, the porosity is kept relatively low. Unfortunately, the Richardson-Zaki index models tends to overestimate the minimum fluidisation velocity and therefore also results in less accurate predictions with respect to porosity values. We extended the Richardson-Zaki model with proven hydraulics-based models. The minimum fluidisation velocity is acquired using the model proposed by Kozeny (1927), Ergun (1952) and Carman (1937). The terminal settling velocity is obtained through the model developed by Brown & Lawler (2003), which is an improved version of the well-known model developed by Schiller & Naumann (1933). The proposed models are compared with data from expansion experiments with calcium carbonate grains, crushed calcite and garnet grains applied in drinking water softening using the fluidised bed process. With respect to porosity, prediction accuracy is improved, with the average relative error decreasing from 15% to 3% when the classic Richardson-Zaki model is extended with these hydraulics-based models. With respect to minimum fluidisation velocity, the average relative error decreases from 100% to 12%. In addition, simplified analytical equations are given for a straightforward estimation of the index n.Complex Fluid ProcessingSanitary Engineerin
Improvement of the Richardson-Zaki liquid-solid fluidisation model on the basis of hydraulics (PPT)
No full textOne of the most popular and frequently used models for describing homogeneous liquid-solid fluidised suspensions is the model developed by Richardson & Zaki in 1954. The superficial fluid velocity and terminal settling velocity together with an index, make it possible to determine the fluid porosity in a straightforward way. To maintain optimal process and control conditions in multiphase drinking water treatment processes, the porosity is kept relatively low. Unfortunately, the Richardson-Zaki index models tend to overestimate the minimum fluidisation velocity and therefore also result in less accurate predictions with respect to porosity values. We extended the Richardson-Zaki model with proven hydraulics-based models. The proposed models are compared with data from expansion experiments with grains applied in drinking water softening using the fluidised bed process. The porosity prediction error decreases from 15% to 3% and the minimum fluidisation velocity error from 100% to 12%.Sanitary Engineerin
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