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Utilizzo di un microarray (microPEACH1.0) per lo studio del trascrittoma durante la maturazione della pesca
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Characterization and expression of two genes encoding ethylene receptors in peach fruit
No full textWe have isolated two peach (Prunus persica) genes, Pp-ETR1 and Pp-ERS1, homolog to the Arabidopsis ethylene receptor ETR1 and ERS1. Pp-ETR1 is identical, in terms of exons number and introns position, to At-ETR1 although the first and fifth intron are 5 and 20 times longer, respectively. In addition two putative polyadenilation sites, that may cause an incomplete splicing at the 3' terminus, are present into the fifth intron. Translation of such a truncated transcript would lead to a product missing a large portion of the receiver domain. The coding region of Pp-ERS1 is organized in five exons interrupted by four introns, but unlike Pp-ETR1 no marked differences in terms of intron length between At-ERS1 and Pp-ERS1 have been detected. Into the promoter region of Pp-ERS1 a motif of 28 nt, which shows high homology with binding ethylene-factors detected in genes up-regulated by ethylene, is present. The deduced protein of both genes contain a sensor domain and the Histidine kinase domain, in which residues, thought to be important for the normal function of ETR1 and ERS-type proteins as ethylene receptors are conserved. These results indicate that Pp-ETR1 and Pp-ERS1 could be functional ethylene receptors with the ability to bind ethylene. Expression analysis, carried out by quantitative RT-PCR, was performed during fruit ripening (cv Maria Marta). The level of Pp-ETR1 transcripts remained unchanged throughout ripening, whereas Pp-ERS1 mRNA increased in parallel with the ethylene climacteric
Transcriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP
No full textA large-scale transcriptome analysis has been conducted using mPEACH1.0 microarray on nectarine (Prunus persica L. Batsch) fruit treated with 1-methylcyclopropene (1-MCP). 1-MCP maintained flesh firmness but did not block ethylene biosynthesis. Compared with samples at harvest, only nine genes appeared to be differentially expressed when fruit were sampled immediately after treatment, while a total of 90 targets were up- or down-regulated in untreated fruit. The effect of 1-MCP was confirmed by a direct comparison of transcript profiles in treated and untreated fruit after 24 h of incubation with 106 targets differentially expressed. About 30% of these targets correspond to genes involved in primary metabolism and response processes related to ethylene, auxin, and other hormones. In treated fruit, altered transcript accumulation was detected for some genes with a role in ripening-related events such as softening, colour development, and sugar metabolism. A rapid decrease in flesh firmness and an increase in ethylene production were observed in treated fruit maintained for 48 h in air at 20 oC after the end of the incubation period. Microarray comparison of this sample with untreated fruit 24 h after harvest revealed that about 45% of the genes affected by 1-MCP at the end of the incubation period changed their expression during the following 48 h in air. Among these genes, an ethylene receptor (ETR2) and three ethylene responsive factors (ERF) were present, together with other transcription factors and ethylene-dependent genes involved in quality parameter changes
Caratterizzazione ed identificazione genetica di alcune cultivar di olivo presenti nei Colli Euganei
No full textAFLP (Amplified Fragment Length Polymorphism) analysis has been used to study the genetic diversity of some olive (Olea europaea L.) trees present in the Colli Euganei area, located in the Northern limit for olive cultivation in Italy. The genetic identification of autochthonous plants diffused in this area is difficult and, up to now, few genotypes have been distinguished by comparing morphological characters and some physiological behaviours. The AFLP analysis has been performed on DNA samples collected from 20 trees selected among four of these ecotypes. The dendrogram clearly shows two main distinct groups: one group includes all the genotypes empirically defined as Rasara, which show similar morphological characters, and the Marzamina ecotype. The second cluster contains the other two genotypes (Matosso and unnamed), distinguished between them at similarity level of about 0.60
Characterization and expression of two genes encoding ethylene receptors in peach fruits
No full textTranscriptome profiling of ripening nectarine (Prunus persica L. Batsch) fruit treated with 1-MCP
Full text linkA large-scale transcriptome analysis has been conducted using μPEACH1.0 microarray on nectarine (Prunus persica L. Batsch) fruit treated with 1-methylcyclopropene (1-MCP). 1-MCP maintained flesh firmness but did not block ethylene biosynthesis. Compared with samples at harvest, only nine genes appeared to be differentially expressed when fruit were sampled immediately after treatment, while a total of 90 targets were up- or down-regulated in untreated fruit. The effect of 1-MCP was confirmed by a direct comparison of transcript profiles in treated and untreated fruit after 24 h of incubation with 106 targets differentially expressed. About 30% of these targets correspond to genes involved in primary metabolism and response processes related to ethylene, auxin, and other hormones. In treated fruit, altered transcript accumulation was detected for some genes with a role in ripening-related events such as softening, colour development, and sugar metabolism. A rapid decrease in flesh firmness and an increase in ethylene production were observed in treated fruit maintained for 48 h in air at 20 °C after the end of the incubation period. Microarray comparison of this sample with untreated fruit 24 h after harvest revealed that about 45% of the genes affected by 1-MCP at the end of the incubation period changed their expression during the following 48 h in air. Among these genes, an ethylene receptor (ETR2) and three ethylene-responsive factors (ERF) were present, together with other transcription factors and ethylene-dependent genes involved in quality parameter changes
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