2 research outputs found

    Abscisic acid as an internal integrator of multiple physiological processes modulates leaf senescence onset in Arabidopsis thaliana

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    Many studies have shown that exogenous abscisic acid (ABA) promotes leaf abscission and senescence. However, owing to a lack of genetic evidence, ABA function in plant senescence has not been clearly defined. Here, two-leaf early-senescence mutants (eas) that were screened by chlorophyll fluorescence imaging and named eas1-1 and eas1-2 showed high photosynthetic capacity in the early stage of plant growth compared with the wild type. Gene mapping showed that eas1-1 and eas1-2 are two novel ABA2 allelic mutants. Under unstressed conditions, the eas1 mutations caused plant dwarf, early germination, larger stomatal apertures, and early leaf senescence compared with those of the wild type. Flow cytometry assays showed that the cell apoptosis rate in eas1 mutant leaves was higher than that of the wild type after day 30. A significant increase in the transcript levels of several senescence-associated genes, especially SAG12, was observed in eas1 mutant plants in the early stage of plant growth. More importantly, ABA-activated calcium channel activity in plasma membrane and induced the increase of cytoplasmic calcium concentration in guard cells are suppressed due to the mutation of EAS1. In contrast, the eas1 mutants lost chlorophyll and ion leakage significant faster than in the wild type under treatment with calcium channel blocker. Hence, our results indicate that endogenous ABA level is an important factor controlling the onset of leaf senescence through Ca2+ signaling

    A Performance Practice Guide for :only.just.almost.never for Solo Vibraphone by Jay Alan Yim

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    Abstract Currently, there exists little scholarship on Dr. Jay Alan Yim’s percussion music, and none exists for :only.just.almost.never for solo vibraphone. Establishing a performance practice guide will allow future percussionists a glimpse into the compositional process of Yim and allow them to expand their repertoire to include this challenging music. The purpose of this project is to create a comprehensive performance practice guide for :only.just.almost.never for solo vibraphone by Jay Alan Yim. Resources Required This project relies mainly on the score of :only.just.almost.never for solo vibraphone. I have also conducted multiple interviews with Yim regarding his compositional process, sketches, and companion pieces within this writing paradigm. It should be noted that this work can be played with Yim’s other modular works in his cycle for piano,[1] string quartet,[2] woodwind trio,[3] and chamber ensemble;[4] all of these scores are available from the composer. Project Format This project is a written performance practice guide for :only.just.almost.never for solo vibraphone.[5] Thus, it consists of pedagogical advice in learning and performing this work accompanied by specific score examples, visual diagrams, photos, and audio links. In addition, this paper discusses practical analyses of this composition’s complex rhythms. The author has recorded :only.just.almost.never, which has informed the pedagogical writings within this project. [1] Jay Alan Yim, :[ten]dril (Shinkyoku Edition, 1999). [2] Jay Alan Yim, :memo/ryCircle (Shinkyoku Edition, 1998). [3] Jay Alan Yim, :circl/eSong (Shinkyoku Edition, 1997). [4] Jay Alan Yim, :dreamin/gField (Shinkyoku Edition, 1997). [5] Jay Alan Yim, :only.just.almost.never (Shinkyoku Edition, 2018)
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