14 research outputs found
Spastic paraplegia type 31: A novel REEP1 splice site donor variant and expansion of the phenotype variability
No full textMutations in REEP1 have been identified in three types of neurological disorders, autosomal dominant form of Hereditary Spastic Paraplegia type 31 (SPG31), autosomal dominant distal hereditary motor neuronopathy type VB (HMN5B), and autosomal recessive form of congenital axonal neuropathy and diaphragmatic palsy. Previous studies demonstrated different molecular pathogenesis in SPG31, including loss-of-function, gain-of-function and haploinsufficiency. A four-generation family from Japan, including 12 members, was investigated clinically and genetically. Seven affected members displayed pure spastic paraplegia. Impression of genetic anticipation was observed in the family, including tendency of earlier age-at-onset and increasing severity in subsequent generations. Genetic analysis revealed a heterozygous intronic variant, c.303+2T > A, in REEP1, which segregated with disease, and was also identified in one unaffected member. The variant causes exon 4 skipping leading to frame shift and a truncated transcript identified by complementary DNA sequencing of reverse transcription polymerase chain reaction products. Measurement of REEP1 transcripts in lymphocytes demonstrated a reduction through nonsense mediated mRNA decay (NMD). Our study demonstrated further evidence of allelic heterogeneity in SPG31, mutant REEP1 mRNA dosage effects through NMD and intra-familial phenotype variability
Indirect activation of pregnane X receptor in the induction of hepatic CYP3A11 by high-dose rifampicin in mice
No full textJ1620202 Gait Analysis of Knee Osteoarthritis Patients by Using Approximate Plane of Elevation Angle : Confirmation of a Planar Law and Analysis of Angular Difference of the Approximate Plane
No full textHeterogeneous effects of cytotoxic chemotherapies for platinum-resistant ovarian cancer
No full textBackground: Single-agent chemotherapy with or without bevacizumab (Bev) is a standard therapy for platinum-resistant ovarian cancer (PR-OC). However, there is a lack of literature on chemotherapy agent selection in heterogenous PR-OC. Therefore, we aimed to clarify the heterogeneous treatment effects of each chemotherapy agent. Methods: Patients who underwent single-drug chemotherapy agents or Bev combination therapy for PR-OC between January 2009 and June 2022 were included in this study. We assessed the impact of each chemotherapy agent on the time to treatment failure (TTF) according to histological type, platinum-free interval (PFI), and Bev usage. Results: A total of 158 patients received 343 different chemotherapy regimens. In patients with clear cell carcinoma/mucinous carcinoma (CC/MC), gemcitabine (GEM) had the strongest effect with a median TTF of 5.3 months, whilst nedaplatin (NDP) had the lowest effect with a median TTF of 1.4 months. In contrast, in the non-CC/MC group, irinotecan (CPT-11) and NDP had a better TTF than GEM and pegylated liposomal doxorubicin (PLD). There were notable differences in the treatment efficacy of NDP according to PFI. Specifically, NDP prolonged the TTF in patients with a PFI ≥ 3 months. Compared with GEM alone, GEM + Bev tended to prolong the TTF more effectively; however, an additive effect was not observed with PLD + Bev. Conclusions: This study demonstrated that the effect of chemotherapy agents differed according to the tumor and background characteristics of the patient. Our findings will improve selection of effective therapies for patients with PR-OC by considering their background characteristics. © 2023, The Author(s).Open access articleThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Preparation of Tenuifolin from Polygala senega L. Root Using a Hydrolytic Continuous Flow System under High-Temperature High-Pressure Conditions
No full textAn
improved process for preparing tenuifolin (presenegenin 3-β-d-glucopyranoside) from the root of Polygala senega L. was developed. A crude saponin mixture extracted from P. senega was subjected to hydrolysis, and the reactivity
of compounds in the extract was controlled by utilizing the combination
of a flow reactor and experimental design. In addition, column chromatography
with HP 20, a synthetic polystyrenic adsorbent, allowed the gram-scale
preparation of tenuifolin in a continuous manner with fewer steps.
This approach shortens the total time required for gram-scale preparation
from 16 to 5 h in a continuous manner while improving the yield from
0.59% to 2.08% (w/w)
Preparation of Tenuifolin from <i>Polygala senega</i> L. Root Using a Hydrolytic Continuous Flow System under High-Temperature, High-Pressure Conditions
No full textAn
improved process for preparing tenuifolin (presenegenin 3-β-d-glucopyranoside) from the root of Polygala senega L. was developed. A crude saponin mixture extracted from P. senega was subjected to hydrolysis, and the reactivity
of compounds in the extract was controlled by utilizing the combination
of a flow reactor and experimental design. In addition, column chromatography
with HP 20, a synthetic polystyrenic adsorbent, allowed the gram-scale
preparation of tenuifolin in a continuous manner with fewer steps.
This approach shortens the total time required for gram-scale preparation
from 16 to 5 h in a continuous manner while improving the yield from
0.59% to 2.08% (w/w)
Preparation of Tenuifolin from <i>Polygala senega</i> L. Root Using a Hydrolytic Continuous Flow System under High-Temperature, High-Pressure Conditions
No full textAn
improved process for preparing tenuifolin (presenegenin 3-β-d-glucopyranoside) from the root of Polygala senega L. was developed. A crude saponin mixture extracted from P. senega was subjected to hydrolysis, and the reactivity
of compounds in the extract was controlled by utilizing the combination
of a flow reactor and experimental design. In addition, column chromatography
with HP 20, a synthetic polystyrenic adsorbent, allowed the gram-scale
preparation of tenuifolin in a continuous manner with fewer steps.
This approach shortens the total time required for gram-scale preparation
from 16 to 5 h in a continuous manner while improving the yield from
0.59% to 2.08% (w/w)
Preparation of Tenuifolin from <i>Polygala senega</i> L. Root Using a Hydrolytic Continuous Flow System under High-Temperature, High-Pressure Conditions
No full textAn
improved process for preparing tenuifolin (presenegenin 3-β-d-glucopyranoside) from the root of Polygala senega L. was developed. A crude saponin mixture extracted from P. senega was subjected to hydrolysis, and the reactivity
of compounds in the extract was controlled by utilizing the combination
of a flow reactor and experimental design. In addition, column chromatography
with HP 20, a synthetic polystyrenic adsorbent, allowed the gram-scale
preparation of tenuifolin in a continuous manner with fewer steps.
This approach shortens the total time required for gram-scale preparation
from 16 to 5 h in a continuous manner while improving the yield from
0.59% to 2.08% (w/w)
Preparation of Tenuifolin from <i>Polygala senega</i> L. Root Using a Hydrolytic Continuous Flow System under High-Temperature, High-Pressure Conditions
No full textAn
improved process for preparing tenuifolin (presenegenin 3-β-d-glucopyranoside) from the root of Polygala senega L. was developed. A crude saponin mixture extracted from P. senega was subjected to hydrolysis, and the reactivity
of compounds in the extract was controlled by utilizing the combination
of a flow reactor and experimental design. In addition, column chromatography
with HP 20, a synthetic polystyrenic adsorbent, allowed the gram-scale
preparation of tenuifolin in a continuous manner with fewer steps.
This approach shortens the total time required for gram-scale preparation
from 16 to 5 h in a continuous manner while improving the yield from
0.59% to 2.08% (w/w)
Preparation of Tenuifolin from <i>Polygala senega</i> L. Root Using a Hydrolytic Continuous Flow System under High-Temperature, High-Pressure Conditions
No full textAn
improved process for preparing tenuifolin (presenegenin 3-β-d-glucopyranoside) from the root of Polygala senega L. was developed. A crude saponin mixture extracted from P. senega was subjected to hydrolysis, and the reactivity
of compounds in the extract was controlled by utilizing the combination
of a flow reactor and experimental design. In addition, column chromatography
with HP 20, a synthetic polystyrenic adsorbent, allowed the gram-scale
preparation of tenuifolin in a continuous manner with fewer steps.
This approach shortens the total time required for gram-scale preparation
from 16 to 5 h in a continuous manner while improving the yield from
0.59% to 2.08% (w/w)
