82,842 research outputs found
An extension of min/max flow framework
No full textIn this paper, the min/max flow scheme for image restoration is revised. The novelty consists of the fol-
24 lowing three parts. The first is to analyze the reason of the speckle generation and then to modify the
25 original scheme. The second is to point out that the continued application of this scheme cannot result
26 in an adaptive stopping of the curvature flow. This is followed by modifications of the original scheme
27 through the introduction of the Gradient Vector Flow (GVF) field and the zero-crossing detector, so as
28 to control the smoothing effect. Our experimental results with image restoration show that the proposed
29 schemes can reach a steady state solution while preserving the essential structures of objects. The third is
30 to extend the min/max flow scheme to deal with the boundary leaking problem, which is indeed an
31 intrinsic shortcoming of the familiar geodesic active contour model. The min/max flow framework pro-
32 vides us with an effective way to approximate the optimal solution. From an implementation point of
33 view, this extended scheme makes the speed function simpler and more flexible. The experimental
34 results of segmentation and region tracking show that the boundary leaking problem can be effectively
35 suppressed
Min-yu Chen, piano, April 19, 2017
Full text linkThis is the concert program of the Min-yu Chen, piano performance on Wednesday, April 19, 2017 at 8:00 p.m., at the Concert Hall, 855 Commonwealth Avenue. Works performed were Cinq mélodies populaires grecques by Maurice Ravel, Don Quichotte à Dulcinée by M. Ravel, Fantasiestücke Op. 73 by Robert Schumann, and Violin Sonata No. 2 in A major, Op. 100 by Johannes Brahms. Digitization for Boston University Concert Programs was supported by the Boston University Humanities Library Endowed Fund
Min qiang ti yu hui
No full text題名據原刊物封面.內文刊載黃漢勛所著之"練武十則", "螳螂派拳術起源與系統", 及"七星螳螂躱剛拳名稱".Ti ming ju yuan kan wu feng mian.Nei wen kan zai Huang Hanxun suo zhu zhi "Lian wu shi ze", "Tang lang pai quan shu qi yuan yu xi tong", ji "Qi xing tang lang duo gang quan ming cheng"
Exporting New City Developments? From New Towns to Smart Cities
No full text1. Introduction: Reconsidering the Korean Urban Development Experience for International Cooperation (Se Hoon Park, Hyun Bang Shin, and Hyun Soo Kang)
Part I: Outlining the Urban Transformation of Korea
2. Transformations in the Governance of Urban and Regional Planning in Korea: From (Neo-)Developmentalism to Civic Democracy, 1965–2020 (Mike Douglass)
3. Korea’s Regional Development Policy: Understanding Its Context and Drawing Implications for International Development Cooperation (Won Bae Kim)
4. Urban Transformation with ‘Korean Style’: Lessons from Property-based Urban Development (Hyun Bang Shin)
5. From Commodities to Community Engagement: Localities and Urban Development in Seoul, Korea (Blaž Križnik and Su Kim)
Part II: Modeling the Korean Urban Development Experience
6. Export Urbanism: Asian Emerging Donors and the Politics of Urban Development Knowledge Sharing (Jamie Doucette and Farwa Sial)
7. A Multitude of Models: Transferring Knowledge of the Korean Development Experience (Cuz Potter and Jinhee Park)
8. International Urban Development Leadership: Singapore, China and South Korea Compared (Hyung Min Kim, Julie Miao, and Nicholas Phelps)
Part III: Policies and Institutions of the Korean Urban Development
9. Exporting New City Developments? From New Towns to Smart Cities (Yu Min Joo)
10. Housing Policy and Urban Redevelopment in Contemporary Korea (Seong Kyu Ha)
11. Land Development Schemes in South Korea: Background, Structure and Outcome (Seowhan Lim)
12. Knowledge-Policy Nexus: Policy Research Institutes and the Urban Development Regime in Korea (Se Hoon Park)
13. Engines for Development: Public Development Corporations and Their Role in Urban Development in Korea (Jieun Kim)TRU
Min-yu Chen, collaborative piano, April 19, 2018
Full text linkThis is the concert program of the Min-yu Chen, collaborative piano performance on Thursday, April 19, 2018 at 8:00 p.m., at the Concert Hall, 855 Commonwealth Avenue. Works performed were Hermit Songs by Samuel Osborne Barber II, Vier Lieder, Op. 27 by Richard Strauss, and Piano Trio in A minor by Maurice Ravel. Digitization for Boston University Concert Programs was supported by the Boston University Humanities Library Endowed Fund
Study of E. coli Lon protease with its oligomerization, substrate recognition, and screen of genomic library for its candidate substrate in yeast two-hybrid system
No full textLon蛋白酶是一種 ATP-dependent protease，普遍地存在於各種原核生物及真核生物粒線體中。同時Lon在演化上也具有高度的保留性，由此可見其在生物體內的重要。至於 ATP-dependent protease 在功能方面，它能夠分解細胞內不正常累積的蛋白質，或是將折疊錯誤的蛋白質回復成正常構形。最近有相關的研究指出，位於大腸桿菌中的 Lon C 端的P domain(proteolytic domain)，主要是負責蛋白酶間的聚合 (oligomerization)，而辨識基質的部份則位於N端 (N domain)。但在 Brevibacillus thermoruber 中，則有人認為負責聚合的位置在Lon的N端。雖然在本研究中，是使用大腸桿菌 K12 作為研究的材料。但由於序列保留性高的關係，在其他菌種的資料也值得作為參考。
在大腸桿菌中，有已知 Lon 的主要的基質為－ SulA、RcsA，而Lon 蛋白酶本身辨識基質的確切位置卻仍不甚了解。在本論文中，首先將 Lon 和已知的基質以酵母菌雙雜合系統做測試，結果發現 Lon 和 SulA 有交互作用。爲了找出 Lon 蛋白酶辨識 SulA 的區域以及本身聚合的位置，於是將Lon蛋白酶及其 N 區塊、P 區塊及基質於此系統當中表現，配合前人的研究以找出可能蛋白－蛋白的位置，針對特定的區塊作隨機性的突變。隨後以 SulA 篩選出含有對於辨識基質有影響突變點的轉殖菌株－ Lon30、Lon63 以及 Lon66。藉由 β-galactosidase assay 及 MMS (Methyl Methanesulfonate) test 的測試，發現在胺基酸序列 S307R 突變的位置，對於Lon 蛋白酶辨識 SulA 有較明顯的影響，佐證了之前的推測。另外，有研究指出 SulA 蛋白末端的八個胺基酸對於 Lon 的辨識有很大的影響，其中又以最後的ㄧ個胺基酸Histidine為最，不過在 SulA deletion 的實驗當中發現，僅僅去除掉 C 端15個胺基酸還是看的到蛋白質之間的交互作用，而要去除 C 端40個胺基酸才會對辨識有顯著的影響。此外，在酵母菌雙雜合系統中，以Lon蛋白酶篩選整個大腸桿菌的基因庫之研究，發現在挑到的蛋白質基因序列當中，都沒有任何有相似性的共識序列，因此推論 Lon 蛋白酶可能藉由特定的結合位置，對很多種不同序列的小分子蛋白作用。Lon is an ATP-dependent protease found in most organisms. The DNA sequence of Lon is highly conserved in evolution. Lon protease of Escherichia coli regulates a diverse set of physiological responses including cell division, capsule production, plasmid stability, and phage replication. Lon protease and members of Clp family of molecular chaperons and protease regulatory subunits contain homologous regions with properties expected for substrate-binding domain. Recently the crystal structure of the proteolytic domain (P domain) of Escherichia coli Lon has been solved. The proteolytic domain (P domain) has a unique fold and assembles into hexameric rings, and the substrate recognition site locates in the N domain. But in the Brevibacillus thermoruber, N domain assembles the Lon protease to become an active oligomer.
Several substrates of Lon protease have been known in Escherichia coli－including SulA and RcsA. However, the precise position of substrate-recognizing site in Lon protease is still not clear yet. In this study, an interaction between Lon protease and SulA protein was demonstrated by yeast two-hybrid system. For finding the SulA-recognizing site and the Lon-Lon oligomerizing position in Lon, protein-protein interactions between Lon 、N domain、P domain and SulA were also tested here. From our observations and previous data, we hypothesize that SulA recognition site of Lon is located at N domain. Lon mutants (30Lon, 63Lon, 66Lon) for alteration of substrate binding were found by screening the mutagenized Lon proteins. Based on the results of the β-galactosidase assay and MMS (Methyl Methanesulfonate) test in Escherichia coli, we showed that Lon mutant S307R reduces the SulA-binding affinity. It has been reported that the C-terminal eight residues of SulA is recognized by Lon protease, and the last one amino acid Histidine is specifically necessary. However in our studies, the protein-protein interaction can yet be detected when we deleted 15 residues from the C-terminal of SulA protein. Only the last 40 residues deleted from the C-terminal of SulA showed an influence on the recognition and binding affinity with Lon. Finally, fish to the conserved regions of Lon specific-substrates, respectively screening the E.coli genomic library has been done in this study. By using the above similar strategy, several small molecules interacted with Lon have been found. However, the results indicate that no conserved region interacted with Lon has been found among all the isolated proteins. We suggest that Lon protease may use an unique site for binding with the small proteins that we identified.Abstract I
中文摘要 III
壹、前言 1
一、ATP-dependent protease (ATP 依賴蛋白酶) 1
二、Single-chain protease－Lon protease 2
三、Substrates of Lon protease－SulA、RcsA 3
四、研究目的 4
貳、材料與方法 6
一、實驗系統：酵母菌雙雜合系統 (Yeast two-hybrid system) 6
二、實驗材料 7
1、實驗菌株及質體 7
2、培養基 7
3、實驗相關核酸引子 9
4、相關試劑及藥品 9
5、實驗相關套組 9
6、儀器 10
三、實驗方法 10
1、Mini-preparation of plasmid DNA from bacteria 10
2、Preparation of plasmid DNA from yeast 10
3、Preparation of genomic DNA from bacteria 11
4、PCR(Polymerase Chain Reaction) 12
5、Preparation of competent cell 13
6、Transformation 14
7、Error prone PCR(To mutagenize the 195-508 amino acid region of Lon pretease ) 16
8、Site directed mutagenesis 16
9、Screening Lon mutants with SulA in yeast two-hybrid system 17
10、Analysis of reporter gene in yeast 18
11、cps-lacZ expression: β-galactosidase assay in bacteria 19
12、SulA degradation test: MMS assay 20
13、SDS蛋白質膠體電泳 20
14、Western Blotting 22
參、實驗結果 25
一、利用酵母菌雙雜合系統，測試 Lon 蛋白酶和其基質 SulA 蛋白之間的交互作用。 25
二、在酵母菌雙雜合系統中，SulA 與Lon蛋白酶及其 N 、P domain 與基質之間交互作用之表現。 26
三、利用酵母菌雙雜合系統篩選Lon蛋白酶突變株。 27
四、大腸桿菌中，Lon蛋白酶突變株測試。 28
五、SulA蛋白質受到Lon蛋白酶辨識位置之研究。 29
六、利用酵母菌雙雜合系統篩選大腸桿菌基因庫中受到Lon蛋白酶辨識之蛋白基因序列。 30
肆、討論 31
ㄧ、在酵母菌中，以雙雜交系統觀察 Lon 蛋白酶和其基質的作用。 31
二、 Lon 蛋白酶 N 端、 P domain 與基質間交互作用之探討。 31
三、初窺 Lon 蛋白酶辨識基質的區域。 32
四、SulA 蛋白本身受到 Lon 蛋白酶辨識的區域。 33
五、篩選大腸桿菌基因庫，尋找 Lon 蛋白酶可辨識之 DNA 序列相似性。 33
六、討論RcsA 蛋白於酵母菌雙雜交系統中，和 Lon 蛋白酶沒有作用的可能性。 34
伍、參考文獻 36
陸、表 38
柒、圖 4
Two Faces of Mega-Urban Region Formation in South Korea
No full textThis book discusses lessons and challenges of metropolitan circles development and urbanization in Asia, Europe, North America, Latin America and Africa. The book examines the effects of local governance systems, central-local relations, and administrative borders on metropolitan area development. It surveys economic, social and environmental issues, with an emphasis on how interconnectivity, circular economy, and climate issues should be integrated into megaregion development planning.
The chapters are selected papers from the international conference on metropolitan circles development and urbanization jointly held by the Institute of Public Policy (IPP) at the South China University of Technology and UNESCO in 2018. Contributors from the US, the UK, Japan, France, Singapore, Indonesia, Mexico, Tanzania present their questions, observations, and analyses in a narrative and descriptive style which appeal to a wide range of audience.About the Editors
Introduction (Hans d'Orville)
Governance Structure:
Thriving Tokyo and Declining Osaka: The Role of the Local Governance System (Yoshihisa Godo)
How Wide is the Hudson River? The Effect of the State Border on the New York–New Jersey Metropolitan Region (Brendan O'Flaherty)
Metropolitan Circles: Experience from the UK (Cliff Hague)
Planning Mega-Regions in China and India (Xuefei Ren)
Urban Economy and Sustainability:
Land Redevelopment and the Built Environment in Third-Wave Cities: Review and Synthesis (Allen J Scott)
Circular Economy and Metropolitan Circles in Europe — Case Study: Amsterdam (Mehri Madarshahi)
Current Conditions and Future Drivers of Mega-City Regions in the US, Focusing on the Los Angeles Metropolitan Area (Hilda Blanco)
Linking the European SUMP Method to Strasbourg and Paris Metropolitan Circle Scenarios (Cristiana Mazzoni and Andreea Grigorovschi)
Emerging Metropolitan Circles and Urbanization:
Two Faces of Mega-Urban Region Formation in South Korea (Yu-Min Joo)
The Growing Megapolis Jakarta: Urbanization, Cities-Integration, and Future Issues (Gumilar Rusliwa Somantri)
Mexico: Metropolitan Circles Development, Interactive Cities, and Future of Urbanization (Clemente Ruiz Durán)
Urbanization in Africa: Commonalities and Departures (Humphrey P B Moshi)
IndexTRU
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