131 research outputs found
Magnetic driven alginate nanoparticles for targeted drug delivery
No full textThe aim of this paper is to develop highly magnetized, biodegradable and biocompatible, polymeric nanoparticles for drug delivery intell therapy. Alginate magnetic nanoparticles are realized by an emulsion/reticulation technique, after the dispersion of magnetite in an alginate solution. Such nanoparticles are characterized in terms of external morphology (FIB imaging), microstructure (TEM imaging), size distribution, zeta potential, magnetic properties (SQUID analysis) and drug release behaviour. Magnetization curves hsow the typical trend of superparamagnetic materials. Important parameters, such as magnetic permeability and magnetic momentum, are derived by employing Langevin theory. Experimental results reveal that a bi-exponential model fully describes the drug release. Finally, in vitro experiments on NIH/3T3 cells are carried out and demonstrate that our magnetic alginate nanoparticles can effectively drive the drug delivery towards an external magnetic field source
Boron Nitride Nanotubes:A Novel Vector for Targeted Magnetic Drug Delivery
No full textWhereas several biomedical applications of carbon nanotubes have been proposed, the use of boron nitride nanotubes (BNNTs) in this field has been largely unexplored despite their unique and potentially useful properties. Our group has recently initiated an experimental program aimed at the exploration of the interactions between BNNTs and living cells. In the present paper, we report on the magnetic properties of BNNTs containing Fe catalysts which confirm the feasibility for their use as nanovectors for targeted drug delivery. The magnetisation curves of BNNTs characterised by the present study are typical of superparamagnetic materials with important parameters, including magnetic permeability and magnetic momentum, derived by employing Langevin theory. In-vitro tests have demonstrated the feasibility for influencing the uptake of BNNTs by living cells by exposure to an external magnetic source. A finite element method analysis devised to predict this effect produced predictive data with close agreement with the experimental observations.</p
Realization, characterization and functionalization of lipidic wrapped carbon nanotubes
No full textMass-produced carbon nanotubes (CNTs) are strongly aggregated and highly hydrophobic, and processes to make them water soluble are required for biological applications. Both covalent and non-covalent strategies are pursued for obtaining stable, highly concentrated CNT aqueous dispersions. Covalent functionalization has the great disadvantage of producing an irreversible chemical modification of nanotubes, thus alterating their mechanical, chemical and electric properties. On the other hand, non-covalent functionalization is often obtained by employing surfactants that sensibly affect cell viability. Moreover, derivatization with biological moieties is often impossible through non-covalent CNT dispersion. This paper proposes a non-covalent dispersion of multi-wall CNT based on a lipidic mixture that can guarantee high concentration and high stability as well as high cytocompatibility. Moreover, CNTs wrapped with a lipid membrane are realized to demonstrate that the proposed CNTs can be functionalised with a dodecapeptide that specifically recognizes activated platelets without chemical modification of the nanotube itself
Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection
No full textWe investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C(16)) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C(16) liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP- positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C(16) lipoplexes, the proportion of GFP- positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C(16), as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection.From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases. (C) 2010 Elsevier Inc. All rights reserved.</p
Wind-tunnel study of Oxford Centre, Pittsburgh
No full textCER80-81JAP-JEC31.Prepared for Hellmuth, Obata & Kassabaum, P.C.CSU Project 2-27180.Includes bibliographical references (page 24).January 1981
Wind-tunnel study of Main Place Hotel, Dallas
No full textCER78-79JAP-JEC44.Prepared for Hellmuth, Obata & Kassabaum, Inc.Includes bibliographical references (page 24).March 1979
Wind-tunnel study of Phillips Petroleum Project, Bartlesville, Oklahoma
No full textCER83-84JAP-JEC43.Includes bibliographical references (page 26).April 1984.CSU Project 2-95760.For Phillips Petroleum Company through Hellmuth, Obata & Kassabaum, Inc
Wind-tunnel study of Southwestern Bell Telephone Company headquarters building, St. Louis
No full textFor Hellmuth, Obata and Kassabaum, Inc.January 1982.CER81-82JAP-JEC36.Includes bibliographical references.CSU Project 2-27800
The pre-history of Shén-t'sê-chün 神策軍
No full textThe Shên-t'se-chün is famous in history for its great inftuence over the politics of the T'ang (唐). It was the most powerful body in the Chin-chun ((禁軍), the Imperial Guards). But originally it had been created not as a detatchment of the Imperial Guards, but only as one of the frontier guards. Why did it come to beIong to the Imperial Guards? The author tries to present an answer, tracing its career through the An-Shih (安史) Rebellion and the Tibetan's invasion of China
アミノ酸型脂質を用いたリポソームによる生体高分子の高効率な細胞内運搬
No full text制度:新 ; 報告番号:甲2822号 ; 学位の種類:博士(工学) ; 授与年月日:2009/3/15 ; 早大学位記番号:新5042tex
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