7,442 research outputs found
Neurocysticercosis Presenting with Epilepsia Partialis Continua: A Clinicopathological Report and Literature Review
No full textDigitized material from "'Shin shinfujin' kaisetsu, sōmokuji, sakuin"
No full textTables of contents and author index for the eight-volume set Shin shin fujin.The Mellon Foundation - Council on East Asian Libraries Innovation Grants for East Asian Librarian
Dr. Joe Hoyle – Faculty Author Interview
No full textDr. Joe Hoyle, Associate Professor of Accounting in the E. Claiborne Robins School of Business, discusses Introduction to Financial Accounting, a unique online textbook that incorporates many different learning and media techniques. By offering introductory videos, embedded multiple-choice questions and real-life interviews with an investment manager, Hoyle and his co-author include something for every student. The book will be published by Flat World Knowledge in early 2010
The Role of Toll-like Receptor 8 in Ischemic Stroke: Based on a Modified Mouse Stroke Model
No full text背景：目前認為Toll-like receptors(TLRs)可能在缺血性中風的局部發炎反應之源頭扮演一個決定性的角色。TLRs不僅與先天免疫(innate immunity)及後天免疫(adaptive immunity)有關，體內細胞的傷害也會引發其作用。在腦部，神經膠細胞與神經元細胞皆會表現數種TLRs，目前已知TLR2和TLR4會加重缺血性中風時的腦細胞傷害。至於TLR8，除了在對抗病毒上有其角色之外，也會促使神經細胞走向apoptosis。然而，TLR8在缺血性腦中風時是否增強表現、並且影響神經元細胞的apoptosis則是未知。目的：在於確認TLR8在缺血性中風時是否有基因表現量的上升，以及TLR8之蛋白質含量是否也會有局部增加之現象。法：以「單側中大腦動脈及雙側總頸動脈暫時阻斷法」作為改良之腦中風小鼠模式，先以TTC染色證實此中風模式之中風位置。再以RT-PCR與real-time quantitative PCR的方式，分析單側中風小鼠之兩側大腦皮質組織的TLR8 mRNA含量，並探討缺血後不同的再灌流時間是否會有不同的基因表現量。此外，以免疫組織化學染色法(immunohistochemistry)確認中風時腦部是否有表現TLR8之蛋白質，以及會表現TLR8的細胞種類。並以Western blot定量TLR8蛋白質之表現量，比較中風側大腦之表現量是否比對側以及對照組小鼠來得高。結果：我們完成了改良的小鼠缺血性腦中風模式，此模式所造成之缺血性中風是位於右側中大腦動脈灌流範圍之大腦皮質區。與只夾右側中大腦動脈之中風模式比較起來，其所造成之梗塞體積大，死亡率較高。在中風小鼠之神經學障礙方面，我們選用Longa 評分法來評估，發現在同樣的缺血時間之下，再灌流時間越長，則神經學症狀越輕微。在TLR8 mRNA 的表現量方面，缺血90分鐘後再灌流(reperfusion) 1小時及6小時者，皆在中風側之大腦皮質有表現量上升之趨勢，且缺血後再灌流6小時者之中風側與sham對照組之差別已達統計顯著意義(p = 0.021)。在免疫組織化學染色法的結果部分，發現再灌流6小時者，腦部缺血性中風區中有許多細胞具有細胞核皺縮之現象，暗示其正在進行細胞凋亡(apoptosis)，且在其細胞質呈現很強的陽性TLR8染色反應；相對的，對側腦部相對應之部位並無類似之TLR8染色反應。而再灌流18小時的腦部，缺血區的細胞數量已經大量減少，殘存的少數細胞大多有細胞核皺縮之現象，且也有陽性TLR8染色反應。此外，也證實了缺血性中風時會表現TLR8之細胞種類包括了 神經元細胞與神經膠細胞。在Western blot的結果中，於再灌流6小時之後，雙側皆會增加表現TLR8，但雙側沒有明顯差別。結論：本研究以小鼠中風模式，發現缺血性中風時，局部腦組織之細胞會增加表現TLR8之mRNA及蛋白質；而且進一步確認中風時會表現TLR8蛋白質之細胞包括了神經元細胞及神經膠細胞。至於TLR8增加表現所代表之意義對神經細胞而言是保護性或是傷害性，則有待更進一步的研究證實。Background: Toll-like receptors (TLRs) had been regard to play an important role in inducing stroke-related focal inflammatory reaction. TLRs are not only related to innate immunity and adaptive immunity, but also activated by damaged cells. In brain, glia cells and neurons can express several types of TLRs, and it has been proved that TLR2 and TLR4 can worsen the ischemic damage during ischemic stroke. As for TLR8, besides its role in defending virus, it can also induce apoptosis as well. However, it is unknown whether TLR8 is upregulated during ischemic stroke and affecting apoptosis of neuron. Aim: The purpose of this study is to evaluate whether TLR8 gene is upregulated during ischemic stroke, and whether the protein level of TLR8 is increased. Methods: In this study, the modified stroke mouse model was created by “transient unilateral middle cerebral artery and bilateral common carotid arteries occlusion method”, and the stroke area was confirmed by TTC stain. The neurological deficit of stroke mice was evaluated by Longa 5 grading method. We analyzed the level of TLR8 mRNA of bilateral cerebral cortex by RT-PCR and real-time quantitative PCR, and compared the genetic expression at different time points of reperfusion. Besides, we used immunohistochemistry to verify whether ischemic stroke increases local expression of TLR8 protein, and to clarify the cell types with increased TLR8 expression. Besides, Western blot was used to evaluate the expression level of TLR8 protein, and to compare whether the expression level at stroke lesion side would be higher than contralateral side and sham-control mice.Results: The modified mouse stroke model was successfully created, which had an infarction at the cerebral cortex of the right middle cerebral artery territory. As compared with the model made by occlusion of the right middle cerebral artery, this modified model had larger infarct volume and higher mortality rate. Longer reperfusion time was associated with fewer neurological deficits. In the analysis of mRNA expression level, there were trends of increased expression in the ipsilateral cerebral cortex of stroke after ischemia for 90 min followed by reperfusion for 1 hour and 6 hours, and the differences between the group with reperfusion for 6 hours and sham-operated group had statistical significance p = 0.021). In the immunohistochemistry, there were many cells in the ischemic region after reperfusion for 6 hours, which was characterized with condensed nuclei indicating apoptosis. These cells also had strong positive TLR8 stain in their cytoplasm. On the contrary, there was no TLR8 stain in the contralateral hemisphere. In the mouse brain with reperfusion for 18 hours, the cellularity in ischemic region reduced markedly, and these residual cells were also characterized by condensed nuclei and positive TLR8 stain. Besides, we also revealed that neurons and glia cell could express TLR8 during ischemic stroke. In the result of Western blot, the brain of mice underwent ischemia and reperfusion for 6 hours had increased expression of TLR8 in bilateral hemispheres, but there was no obvious side-to-side difference.Conclusion: This study revealed that, in the mouse stroke model, the cells in ischemic region had upregulation of mRNA and protein level of TLR8 during ischemic stroke. Furthermore, the study demonstrated that the cell types expressing TLR8 in ischemic stroke were neurons and glia cells. Further study is needed to clarify the function of TLR8 upregulation is protective or damage for neurons.口試委員會審定書 i謝 ii文摘要 iii文摘要 v目錄 ix目錄 x一章 緒論 1.1 研究背景 1.1.1腦中風之臨床治療現況與策略 1.1.2 腦中風之病態生理學 2.1.3 腦中風的缺血-再灌流傷害 3.1.4 Toll-like receptors是細胞受傷與發炎反應之間的橋樑 4.1.5 Toll-like receptors與腦中風之關係 5.1.6 TLR8與神經細胞凋亡相關 7.1.7 TLR8訊息傳遞路徑之研究 7.1.8 TLR8在免疫的角色 9.1.9 小鼠中風模式 9.2 研究目的與假說 11二章 研究方法及步驟 12.1 小鼠腦中風模式 (Mouse stroke model)：單側中大腦動脈及雙側總頸動脈暫時阻斷法 (Transient unilateral MCA and bilateral CCA occlusion method) 12.2 評估小鼠神經學障礙之程度 13.3 評估小鼠缺血性腦中風之範圍 13.4 TLR8 mRNA表現量的測量 13.5 免疫組織化學染色(Immunohistochemistry) 15.6以西方點墨法(Western blot)測量TLR8蛋白質含量 15.7 統計分析 16三章 研究結果 17.1 小鼠腦中風模式之建立 17.2 TLR8基因表現量的測量 19.3 免疫組織化學染色 20.4 以西方點墨法分析蛋白質表現量 21四章 討論 22.1 討論小鼠缺血性腦中風模式 22.2 討論 TLR8 與其他 TLRs 於缺血性腦中風時之表現 23.3 討論缺血性腦中風時 TLR8 mRNA 表現量之改變情形 24.4 討論TLR8 免疫化學染色之結果 25.5 討論西方點墨法之結果 26.6討論缺血性中風時TLR8增加表現所代表之意義 27.7 本研究之限制 27五章 展望 30文論述 31考文獻 5
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[About the author Joe Clark HBSS]
No full textSheet of text describing photographer Joe Clark and his beginnings in Detroit, Michigan that was displayed during an exhibition of Joe Clark, HBSS photography at the Detroit Institute of Arts
Poetry Reading: Sun Yung Shin
No full textJoin us for a poetry reading by Minnesota Book Award winner Sun Yung Shin and discussion on what sanctuary means for the Twin Cities.
The event is co-sponsored with the St. Thomas English Department.
Sun Yung Shin is the editor of A Good Time for the Truth: Race in Minnesota and the author of two previous poetry/essay collections—Rough, and Savageand Skirt Full of Black (both from Coffee House Press). She is also the co-editor of Outsiders Within: Writing on Transracial Adoption, and the author of bilingual illustrated book for children Cooper’s Lesson. She has received artist grants from the Bush Foundation, the Minnesota State Arts Board, the McKnight Foundation, and the Jerome Foundation. She is teaching or has taught at St. Catherine University, Hamline University, Macalester College, the University of Minnesota, the Perpich Center for Arts Education, Intermedia Arts, Minneapolis public schools, and the Loft Literary Center
Joe Warner
No full textJoe Warner, the author of Biscuits and 'Taters, at the Manatee Historical Commission booth at the 1983 Manatee County Fair
Neurocysticercosis Presenting with Epilepsia Partialis Continua: A Clinicopathologic Report and Literature Review
No full textNeurocysticercosis, a disease caused by a tapeworm larva in the central nervous system, is the leading cause of acquired epilepsy in undeveloped regions of the world. In Taiwan, after improvements in sanitation, tapeworm infection became very rare except in mountain areas inhibited by aborigines. However, in recent years, immigration from other Asian countries has increased rapidly, and parasite infection of the central nervous system may again become an important cause of adult-onset epilepsy in clinical practice. Here, we describe a 27-year-old female Thai immigrant who presented with adult-onset epilepsia partialis continua of the right upper extremity. Electroencephalography showed epileptiform discharge in the left central region, while brain magnetic resonance imaging showed a small enhanced lesion in the left premotor cortex. She underwent operation and pathology of the mass revealed a degenerated cysticercus. In this article , we provide detailed neuroimaging findings, pathologic report, and literature review of this parasitic infection of the central nervous system. This case calls for physicians to be aware of cysticercosis as an etiology of adult-onset epilepsy in immigrants from endemic countries
The Running Revolution: Observations and Advice from Joe Henderson
No full textJoe Henderson has been the heartbeat of the running revolution since its beginnings in the early 1970s. Hiz eighth book offers the best of his running and writing career to this point (1980). Best-selling author Dr. George Sheehan says of him, Joe is a deceptively simple writer who makes it look easy. His instincts and intuitions about the running experience give him complete control, complete confidence. When it comes to writing about running, Joe has perfect pitch. Fellow author Jim Fixx adds that Henderson is as good a mentor as anyone could be lucky enough to have.https://openprairie.sdstate.edu/prairiestriders_pubs/1193/thumbnail.jp
Novelist Eugene Burdick
No full text1964 photograph taken by Joe Munroe of famed author Eugene Burdick as he plays racquetball. Burdick was a political science professor at the University of California. His famous works include 'The Ugly American' (1958), 'Fail-Safe' (1962) and 'The 480' (1965). Joe Munroe's career began in 1939 at the Cranbrook Academy of Art. He served in the Air Force during World War II and then joined Cincinnati-based Farm Quarterly magazine. Though raised in Detroit, agriculture became an important subject of Joe's photographs. He moved to California in 1955 and free-lanced, taking magazine assignments and selling his own work
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