237 research outputs found
Supplemental_material_for_DNA_Switch_Toehold_Mediated_DNA_Isothermal_Amplification_for_Dengue_Serotyping_by_Chan,_et_al – Supplemental material for DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping
No full textSupplemental material, Supplemental_material_for_DNA_Switch_Toehold_Mediated_DNA_Isothermal_Amplification_for_Dengue_Serotyping_by_Chan,_et_al for DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping by Soo Khim Chan, Akinori Kuzuya, Yee Siew Choong and Theam Soon Lim in SLAS Discovery</p
Supplementary material - Supplemental material for Chemical Constituents From <i>Endiandra kingiana</i> (Lauraceae) as Potential Inhibitors for Dengue Type 2 NS2B/NS3 Serine Protease and its Molecular Docking
No full textSupplemental material, Supplementary material, for Chemical Constituents From Endiandra kingiana (Lauraceae) as Potential Inhibitors for Dengue Type 2 NS2B/NS3 Serine Protease and its Molecular Docking by Syazreen N. Sulaiman, Maywan Hariono, Haslinda Mohd Salleh, Soon-Lim Chong, Liew Sook Yee, Azeana Zahari, Habibah A. Wahab, Severine Derbré and Khalijah Awang in Natural Product Communications</p
Naïve antibody library derived monoclonal antibody against VP35 of Ebola virus
No full textEbola virus is notorious for causing severe and even deadly haemorrhagic fever in infected humans and non-human primates. The high fatality rate of Ebola virus disease (EVD) has highlighted the need for effective diagnosis and treatment. Two monoclonal antibodies (mAbs) have been approved by USFDA for treatment of EVD. Virus surface glycoprotein is the common target for diagnostic and therapy including vaccines. Even so, VP35, a viral RNA polymerase cofactor and interferon inhibitor could be a potential target to curb EVD. The present work describes the isolation of three mAb clones from a phage-displayed human naïve scFv library against recombinant VP35. The clones showed binding against rVP35 in vitro and inhibition of VP35 in luciferase reporter gene assay. Structural modelling analysis was also carried out to identify the binding interactions involved in the antibody-antigen interaction model. This allows some insight into the "fitness" of the binding pocket between the paratope and target epitope which would be useful for the design of new mAbs through in silico means in the future. In conclusion, the information obtained from the 3 isolated mAbs could be potentially useful in the quest to improve VP35 targeting for therapeutic development in the future
DESA : a design method that reduces system complexity
No full textThis research deals with a fonnal design method called "Design Equations for
Systems Analysis", or DESA. This method reduces system complexity, and has been
demonstrated to effectively reduce the complexity of human-machine systems. It also
increases the usability of products. The author summarized two other design methods
that reduce system complexity - Axiomatic Design (AD) and Design Structure Matrix
(DSM) - and explained their differences from DESA. A case study was then
presented to demonstrate the ability ofAD and DSM to complement each other. Since
DESA builds on AD, it can complement DSM similarly. The author revised the
framework that DESA utilizes. A tenninology was established to define the technical
tenns in DESA. DESA was then employed to design an inspection method that
evaluates usability. It was also employed to reduce the complexity of an objectoriented
software system. These two applications of DESA are presented as case
studies. These two case studies respectively demonstrate that DESA designs for
usability and effectively reduces the complexity of object-oriented software systems.MASTER OF ENGINEERING (MAE
Distributed human computation framework for linked data co-reference resolution
No full textDistributed Human Computation (DHC) is a technique used to solve computational problems by incorporating the collaborative effort of a large number of humans. It is also a solution to AI-complete problems such as natural language processing. The Semantic Web with its root in AI is envisioned to be a decentralised world-wide information space for sharing machine-readable data with minimal integration costs. There are many research problems in the Semantic Web that are considered as AI-complete problems. An example is co-reference resolution, which involves determining whether different URIs refer to the same entity. This is considered to be a significant hurdle to overcome in the realisation of large-scale Semantic Web applications. In this paper, we propose a framework for building a DHC system on top of the Linked Data Cloud to solve various computational problems. To demonstrate the concept, we are focusing on handling the co-reference resolution in the Semantic Web when integrating distributed datasets. The traditional way to solve this problem is to design machine-learning algorithms. However, they are often computationally expensive, error-prone and do not scale. We designed a DHC system named iamResearcher, which solves the scientific publication author identity co-reference problem when integrating distributed bibliographic datasets. In our system, we aggregated 6 million bibliographic data from various publication repositories. Users can sign up to the system to audit and align their own publications, thus solving the co-reference problem in a distributed manner. The aggregated results are published to the Linked Data Cloud
Motion Control Strategies for Smart Floating Cranes
No full textFloating structures have raised interest in the recent years for different applications, from living and farming at sea to renewable energy production. To support the logistics on the floating structures, floating cranes are necessary and their designs are constantly improved. Increasing developments in the automation industry paved the way for automated crane operations. In this work, motion control of a smart crane is presented with particular attention to the performance under wave motion. In this research, a scaled down, two-dimensional mathematical model of a gantry crane is derived using Lagrangian mechanics and DC motors dynamics. This results in a nonlinear system that is capable of simultaneous traversing and hoisting a container. The system is simulated in MATLAB Simulink environment and a proportional-derivative control and a state feedback control are designed and implemented. Their robustness is explored by modelling sensor behavior, external disturbances and floating platform dynamics. Both control strategies were able to keep stability in a disturbed system. During simulation, the sway angles never exceed 10°. Smaller oscillations occurred using the state feedback control. Therefore, it creates a smoother response compared to the proportional derivative control, which ultimately translates to increased safety, turnover rate and durability of the crane.Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Transport Engineering and Logistic
Development of a pico-satellite : sun sensors for attitude determination
No full textFollowing the completion of the micro-satellite XSAT program, and currently waiting for the launch, NTU has started another program to develop a pico- and nano-satellite pair VELOX-I. This program includes many FYP projects working on the various sub-systems of a satellite.
For a satellite to determine and control its location and orientation in space, the Attitude Determination and Control System (ADCS) is an essential sub-system for the task. This project focuses on the sun sensor in the ADCS which determines the sun direction with respect to the vertical axis of the satellite. The sun direction will allow the satellite to orientate itself perpendicular to the sun direction for maximum solar power collection and also to shelter sensitive equipments away from the strong solar radiation.
In this project, hardware prototype has been designed and implemented on PCB. It has been tested using a sun simulator to simulate real parallel sunrays and a 2-axis turn motor to rotate the sensor. The hardware will provide four voltage analog outputs dependent on the sun spot position.
The voltage outputs is first processed by the microcontroller. Subsequently, the digital data is transferred to PC for noise removal using Kalman filter, computation of sunray angle and error calibration based on the analysis of errors caused by mechanical and electrical design.
The accuracy for the sensor is -1.3º to 0.7º for a field-of-view of -30º to 30º, and ±0.7º for a field of view of -25º to 25º. This performance is comparable to commercial products that have accuracies less than 1º. The prototype has also been tested in outdoors under the real sun and proven to function satisfactorily.Bachelor of Engineerin
Differing roles of axiomatic design and design structure matrix in reducing system complexity
No full textNumerical simulation studies of the effect of membrane permeance on mass transfer enhancement driven by transient slip velocity
No full textOne of the most noteworthy achievements in reverse osmosis (RO) efficiency is the improvement in membrane permeance. Although current membranes offer higher permeance (and flux) than older RO membranes, increases in permeate flux are limited by concentration polarisation (CP) and fouling. Therefore, innovation is needed to reduce CP to further increase permeate flux. An unsteady forced slip velocity can disrupt the boundary layer, thus reducing CP. This paper uses Computational Fluid Dynamics (CFD) to analyse the effect of membrane permeance on the resonant frequency for an unsteady forced slip velocity, as well as the resulting mass transfer enhancement. The results show that the resonant frequency of the unsteady forced slip velocity is not affected by the membrane permeance. Although the results show a peak in the mass transfer enhancement factor for permeance values in the range typically used for brackish water, In addition, this thesis found that that at any membrane permeance, slip velocity can increase the maximum shear stress
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