91 research outputs found

    Development of Natural vegetable sanitizer from Thai local herbs

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    Nowadays, food safety becomes public concern, especially in fresh produce; fresh vegetables and fruits. The CDC has been reported at least 7 outbreaks from fresh produce in the past 3 years; for example, Escherichia coli O121 in raw clover sprouts (2014), E. coli O157:H7 in ready-to-eat salads (2013), Salmonella Saintpaul in cucumbers (2013), Salmonella Typhimurium and Newport in cantaloupe (2012), and E. coli O157:H7 in spinach and spring mix salad (2012). Washing, before consuming, is the most important step in order to prevent foodborne outbreak in fresh produces. Therefore, this research was aimed to develop vegetable sanitizer from crude extracts of Acacia concinna (Willd.) D.C (Som-poi), Capsicum annuum (Chili), Cymbopogon citratus (Lemongrass), Citrus hystrix (Kaffir lime) and Chrysanthemum indicum L. (Chrysanthemum) in order to reduce foodborne bacteria in fresh vegetable. These herbs have been reported for their antibacterial activity (Pitinidhipat and Yasurin, 2012; Utami et al., 2012; Saenghiruna and Yasurin, Lazuardi et al., 2013; 2013; Piya-isarakul and Yasurin, 2013; Saenghiruna et al., 2014; Dung et al., 2014). The 18 treatments with 3 different crude extracts percentage (0.5, 1 and 1.5%), 3 different volumes (3, 5, and 10 ml.) and 2 different soaking times (10 and 20 min) were investigated for their antibacterial synergistic activity using Lactuca sataiva (Lettuce) as vegetable model. The MPN, total place count on PCA, BHI agar for Listeria monocytogenes count, and SS agar for Salmonella sp. count were used to evaluate the antibacterial efficiency of vegetable sanitizer. The results showed that treatment 18 (using 10 ml of 1.5% crude extracts with 20 min soaking time) gave the highest bacterial efficiency statistically by vegetable sanitizer comparing with the commercial brand. The MPN number, the total plate count, and the L. monocytogenes count were 1.34 ±0.19 MPN/ml, 1.62 ± 1.02 log CFU/ml, and 1.31 ± 0.40 log CFU/ml, respectively. The Salmonella sp. was not found. The t-test has been done by using SAS on log CFU/ml with P0.05. It was found that crude extract percentage, used volume, and soaking time affected the antibacterial efficiency of this vegetable sanitizer

    Study of pretreatment impacts on sugarcane bagasse and durian peel by using response surface methodology

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    Dissertation (Ph.D. FB)--Assumption University, 2021.Includes bibliography

    The development of riceberry pudding for people with dysphagia

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    Thesis (M.S.)--Assumption University, 2017.Includes bibliography

    A study of feasibility to utilize lignocellulosic biomass as materials for biodiesel production

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    Lignocellulosic biomass is a renewable, inexpensive, and abundant resource with high potential for biofuel production to implement the sustainable energy energy worldwide. The bottleneck of biofuel production is the recalcitration of ligoncellulosic biomass to fermentable sugars. Searching for a novel cellulase, a biocatalyst is one of major challenges to promote biofuel production with economic and environmental friendly. Natural microorganism is the great source of cellulase production. Therefore the objective of this research is to identify thermophilic, rapid, efficient cellulose-degrading bacteria from organic fertilizer, rice field, activated sludge, and rain forest in Thailand. More than 300 isolates were screened at 45°C on carboxy-methyl-cellulose (CMC)-containing media to observe the cellulase activity. Using standard filter paper assay, only 9 isolates (S3-10, S3-20, L2-S1, L2-S2, S3-7, L3-S1, L11-S2, L11-S3, and L11-S4) showed high total cellulase activity among other isolates. All isolates then were cultured in media containing 4 different carbon sources; CMC, filter paper, untreated rice straw, nutrient broth (NB), to see effect of substrates on cellulase production. It showed that L2-S2 in Dubois salts media with rice straw could induce high total cellulase activity to 25.57 umole/mg-protein while L11-S3 in NB could induce the highest total cellulase activity to 27.92 umole/mg- protein. Therefore untreat rice straw and NB represented as an effective inducer for cellulase production. A portion of the 16srDNA genes of cellulase positive isolates were amplified and sequenced, then BLASTed to determine species. The results showed that most of isolates are Bacillus sp
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