198,188 research outputs found
Fibroblasts in head neck squamous cell carcinoma associated with perineural invasion have high level nuclear Yes-Associated Protein (YAP) expression
No full textPaul A. Reynolds, PhD, is supported by the Melville Trust for the Care and cure of Cancer.We retrospectively studied the expression of Yes-associated protein (YAP) using immunohistochemical staining in 10 cases of head and neck squamous cell carcinoma with associated perineural invasion. We find that fibroblasts in areas associated with perineural invasion show higher levels of nuclear YAP compared to fibroblasts in the stroma of normal mucosa, with a median cell count of 35.4 per high-power field in the former and 3.9 in the latter. No differences were observed between the expression of YAP phosphorylated at Ser127 in the tumoral stroma compared to that in the normal mucosa, with a median cell count expression of 4.9 in the former versus 5.0 in the latter. Therefore, a strong and increased nuclear YAP expression in fibroblasts associated with perineural invasion in head and neck squamous cell carcinoma suggests that YAP-mediated transcription programs in these fibroblasts may contribute to perineural invasion.Peer reviewe
carliercomputationallab/stochastic-model-YAP-activation: stochastic-model-YAP-activation
No full text<p>A stochastic model for YAP activation within focal adhesion complexes</p>
<p>This repository contains the required files to run the 3D particle-based stochastic simulation for YAP activation within the focal adhesion complexes.</p>
YAP-CD24-artical-Supplementary Materials.docx
No full textSupplementary Materials for The Hippo-YAP signaling pathway drives CD24-mediated immune evasion in esophageal squamous cell carcinoma via macrophage phagocytosis</p
BivonaUCSF/YAP: Version 1.0.0
No full text<p>First release of code for YAP manuscript, relating to figure 6-7, supplemental figure 5, 11, 10</p>
YAP-silencing fibroblasts attenuate invasiveness in OSCC cells.
No full text(A) The depletion of YAP with 2 different siRNAs was identified by RT-PCR. Representative image is shown. (B) Nuclear/cytoplasmic fraction was isolated from each protein and then western blot was performed to check nuclear/cytoplasmic YAP expression by siRNAs targeting YAP. Lamin B1 was used as a positive control of nuclear protein fraction and β-tubulin was used as a positive control of cytoplasmic protein fraction. (C) Cell proliferation in siCont- and siYAPs-transfected hTERT-hNOFs. Cells (2 x 105) were seeded into well and then counted after 3 days. (D) Representative images of invading OSCC cells toward lower chamber are shown (magnification: 200X, scale bar: 100 μm). (E, F) The bar graphs indicated a quantification of invading YD10B (E) or YD38 (F) OSCC cells as siYAPs-transfected hTERT-hNOFs were placed in the lower chamber by comparison with siCont transfected hTERT-hNOFs. This assay (E and F) was performed in independent triplicate repeats (*p < 0.05; Mann-Whitney U test).</p
hhandika/yap: v0.4.0
No full text<p>New updates:</p>
<ol>
<li>Remove summary stats from yap stats command. Now. available in <a href="https://www.segul.app/">SEGUL.</a></li>
<li>Add file processing progress after each file is processed.</li>
</ol>
Willin, an upstream component of the Hippo signaling pathway, orchestrates mammalian peripheral nerve fibroblasts
No full textWillin/FRMD6 was first identified in the rat sciatic nerve, which is composed of neurons, Schwann cells, and fibroblasts. Willin is an upstream component of the Hippo signaling pathway, which results in the inactivation of the transcriptional coactivator YAP through Ser127 phosphorylation. This in turn suppresses the expression of genes involved in cell growth, proliferation and cancer development ensuring the control of organ size, cell contact inhibition and apoptosis. Here we show that in the mammalian sciatic nerve, Willin is predominantly expressed in fibroblasts and that Willin expression activates the Hippo signaling cascade and induces YAP translocation from the nucleus to the cytoplasm. In addition within these cells, although it inhibits cellular proliferation, Willin expression induces a quicker directional migration towards scratch closure and an increased expression of factors linked to nerve regeneration. These results show that Willin modulates sciatic nerve fibroblast activity indicating that Willin may have a potential role in the regeneration of the peripheral nervous system.Peer reviewe
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