9 research outputs found
Selection of appropriate reference genes for quantitative real-time PCR in Oxytropis ochrocephala Bunge using transcriptome datasets under abiotic stress treatments
No full textBackground: Oxytropis ochrocephala Bunge, an indigenous locoweed species in China, poses great threats to livestock on grasslands. There is a need for further genetic study in the plants per se, for understanding the basis of its acclimation mechanism in various unfavorable environmental conditions and to implement effective control measures. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is the most commonly used method for gene expression analysis. To facilitate gene expression studies and obtain more accurate qRT-PCR data, normalization relative to stable reference genes is required. The aim of this study was to select the most stable reference genes for transcriptional analysis in O. ochrocephala. Results: We selected 12 candidate reference genes, 18S ribosomal RNA (18S RNA), actin2/7 (ACT7), β-actin (ACTB), actin101 (ACT101), actin11 (ACT11), β-tubulin (TUB), α-tubulin (TUA), glyceraldehyde-3-phosphate dehydrogenase-1 (GAPDH1), GAPDH2, metallothionein-like protein (MET), fructose-bisphosphate aldolase (FBA) and histone H3 (HIS), from the transcriptome datasets of O. ochrocephala and determined the suitability by analyzing their expression levels when exposed to a range of abiotic stress conditions. By employing software packages including geNorm, NormFinder and BestKeeper, HIS, ACT7 and ACT101 were assessed as the most suitable set for normalization in all samples. When normalized with the most stable reference genes, the expression patterns of the three target genes were in accordance with those in the transcriptome data, indicating that the reference genes selected in this study are suitable. Conclusions: The study provided appropriate reference genes for accurate normalization in qRT-PCR analysis in O. ochrocephala and emphasized the importance of validating reference genes for gene expression analysis under specific experimental condition. The usage of inappropriate reference gene would cause misinterpretation
Interplay between Microorganisms and Geochemistry in Geological Carbon Storage
No full textCitation: Kirk, MF, Altman, SJ, Santillan, EFU, Bennett, PC (2016) Interplay between microorganisms and geochemistry in geological carbon storage. International Journal of Greenhouse Gas Control 47, 386-395.Researchers at the Center for Frontiers of Subsurface Energy Security (CFSES) have conducted laboratory and modeling studies to better understand the interplay between microorganisms and geochemistry for geological carbon storage (GCS). We provide evidence of microorganisms adapting to high pressure CO2 conditions and identify factors that may influence survival of cells to CO2 stress. Factors that influenced the ability of cells to survive exposure to high-pressure CO2 in our experiments include mineralogy, the permeability of cell walls and/or membranes, intracellular buffering capacity, and whether cells live planktonically or within biofilm. Column experiments show that, following exposure to acidic water, biomass can remain intact in porous media and continue to alter hydraulic conductivity. Our research also shows that geochemical changes triggered by CO2 injection can alter energy available to populations of subsurface anaerobes and that microbial feedbacks on this effect can influence carbon storage. Our research documents the impact of CO2 on microorganisms and in turn, how subsurface microorganisms can influence GCS. We conclude that microbial presence and activities can have important implications for carbon storage and that microorganisms should not be overlooked in further GCS research
The microbial opsin homologue sop1 is involved in Sclerotinia sclerotiorum development and environmental stress response
No full textMicrobial opsins play a crucial role in responses to various environmental signals. Here, we report that the microbial opsin gene sop1 in the necrotrophic phytopathogenic fungus Sclerotinia sclerotiorum was dramatically up-regulated during infection and sclerotial development compared with the vegetative growth stage. Further study showed sop1 was essential for growth, sclerotial development and full virulence of S. sclerotiorum. Sop1-silenced transformants were more sensitive to high salt stress, fungicides and high osmotic stress. However, they were more tolerant to oxidative stress compared with the wild-type strain, suggesting that sop1 is involved in different stress responses and fungicide resistance, which plays a role in the environmental adaptability of S. sclerotiorum. Furthermore, a Delta blast search showed that microbial opsins are not present in animals and almost all higher plants, indicating that as a predicted transmembrane protein, sop1 is a potential drug target for disease control of S. sclerotiorum
Molecular characterization of a bipartite double-stranded RNA virus and its satellite-like RNA co-infecting the phytopathogenic fungus Sclerotinia sclerotiorum
No full textA variety of mycoviruses have been found in Sclerotinia sclerotiorum. In this study, we report a novel mycovirus Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1) that was originally isolated from the hypovirulent strain SCH941 of S. sclerotiorum. SsBRV1 has rigid spherical virions that are ~38 nm in diameter, and three dsRNA segments (dsRNA1, 2 and 3 with lengths of 6.4, 6.0 and 1.7 kbp, respectively) were packaged in the virions. dsRNA1 encodes a cap-pol fusion protein, and dsRNA2 encodes a polyprotein with unknown functions but contributes to the formation of virus particles. The dsRNA3 is dispensable and may be a satellite-like RNA (SatlRNA) of SsBRV1. Although phylogenetic analysis of the RdRp domain demonstrated that SsBRV1 is related to Botrytis porri RNA virus 1 (BpRV1) and Ustilago maydis dsRNA virus-H1 (UmV-H1), the structure proteins of SsBRV1 do not have any significant sequence similarities with other known viral proteins with the exception of those of BpRV1. SsBRV1 carrying dsRNA3 seems to have no obvious effects on the colony morphology, but can significantly reduce the growth rate and virulence of S. sclerotiorum. Notably, a growth hormone receptor binding domain (GHBP, Pfam12772) is detected in ORF2-encoded protein of SsBRV1, which have not been reported in any other viruses. These findings provide new insights into the virus taxonomy, virus evolution and the interactions between SsBRV1 and the fungal hosts
Exploration of microRNAs and their targets engaging in the resistance interaction between wheat and stripe rust
No full textWheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. miRNAs are important regulators, they play very central roles in plant organ development, vegetable phase change and defense responses. In this study, two miRNA libraries from wheat cultivar Xingzi 9104 (XZ) challenged with the avirulent Pst race CYR32 and sterile water were constructed, respectively. A total of 596 miRNA candidates were obtained. 420 wheat-specific candidate miRNAs were screened in adult plants challenged with Pst using microarray-based analyses. We analyzed the abundance of candidate miRNAs, and the levels of a subset of candidate miRNAs were determined by quantitative real time PCR (qRT-PCR). The qRT-PCR results indicated that some miRNAs were involved in the incompatible interaction between wheat and Pst. In addition, we identified some miRNAs differentially expressed in different leaves. Additionally, the target genes of wheat miRNAs were confirmed by using degradome sequencing technology. Most of the annotated target genes are related to signal transduction, energy metabolism, and other functions. We selected some target genes for relative expression analysis using qRT-PCR, and found that RabGAP/TBC domain-containing protein, zinc finger protein and Cysteine-rich receptor-like protein kinase 41 may play important role in the incompatible interaction between XZ and CYR32. Intriguingly, miRNAs and target gene seem to form a complicated regulation network that regulates the wheat-Pst interaction. Our data provide the foundation for evaluating the important regulatory roles of miRNAs in the wheat-Pst interaction
Sink property of metallic glass free surfaces
No full textWhen heated to a temperature close to glass transition temperature, metallic glasses (MGs) begin to crystallize. Under deformation or particle irradiation, crystallization occurs at even lower temperatures. Hence, phase instability represents an application limit for MGs. Here, we report that MG membranes of a few nanometers thickness exhibit properties different from their bulk MG counterparts. The study uses in situ transmission electron microscopy with concurrent heavy ion irradiation and annealing to observe crystallization behaviors of MGs. For relatively thick membranes, ion irradiations introduce excessive free volumes and thus induce nanocrystal formation at a temperature linearly decreasing with increasing ion fluences. For ultra-thin membranes, however, the critical temperature to initiate crystallization is about 100 K higher than the bulk glass transition temperature. Molecular dynamics simulations indicate that this effect is due to the sink property of the surfaces which can effectively remove excessive free volumes. These findings suggest that nanostructured MGs having a higher surface to volume ratio are expected to have higher crystallization resistance, which could pave new paths for materials applications in harsh environments requiring higher stabilities.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351178500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Multidisciplinary SciencesSCI(E)[email protected]; [email protected]
De novo transcriptome assembly of a Chinese locoweed (Oxytropis ochrocephala) species provides insights into genes associated with drought, salinity and cold tolerance
No full textBackground: Locoweeds (toxic Oxytropis and Astraglus species), containing the toxic agent swainsonine, pose serious threats to animal husbandry on grasslands in both China and the US. Some locoweeds have evolved adaptations in order to resist various stress conditions such as drought, salt and cold. As a result they replace other plants in their communities and become an ecological problem. Currently very limited genetic information of locoweeds is available and this hinders our understanding in the molecular basis of their environmental plasticity, and the interaction between locoweeds and their symbiotic swainsonine producing endophytes. Next-generation sequencing provides a means of obtaining transcriptomic sequences in a timely manner, which is particularly useful for non-model plants. In this study, we performed transcriptome sequencing of Oxytropis ochrocephala plants followed by a de nove assembly. Our primary aim was to provide an enriched pool of genetic sequences of an Oxytropis sp. for further locoweed research. Results: Transcriptomes of four different O. ochrocephala samples, from control (CK) plants, and those that had experienced either drought (20% PEG), salt (150 mM NaCl) or cold (4 °C) stress were sequenced using an Illumina Hiseq 2000 platform. From 232,209,506 clean reads 23,220,950,600 (~23 G nucleotides), 182,430 transcripts and 88,942 unigenes were retrieved, with an N50 value of 1,237. Differential expression analysis revealed putative genes encoding heat shock proteins (HSPs) and late embryogenesis abundant (LEA) proteins, enzymes in secondary metabolite and plant hormone biosyntheses, and transcription factors which are involved in stress tolerance in O. ochrocephala. In order to validate our sequencing results, we further analyzed the expression profiles of nine genes by quantitative real-time PCR. Finally, we discuss the possible mechanism of O. ochrocephala’s adaptations to stress environment. Conclusion: Our transcriptome sequencing data present useful genetic information of a locoweed species. This genetic information will underpin further research in elucidating the environmental acclimation mechanism in locoweeds and the endophyte-plant association
0004
No full textL'AILY PALO AI.TO TIMES. WEDNESDAY, JAN. 3. 1917.
PACK FIVE
VARSITY THEATRE
Paramount Program
TONIGHT
tt
fhe Victoria Cross"
A remark.Me characterisation ol the rlasalr and featurlBg MH"
TKI____t„V. tha noted Mar.
•THE UPPER NILE"
i'tiraiuoun.-BurtoQ HolmM Tra. el Picture.
l',--!l»!i>\ is CENTS,
MA TINKK S:tt> -a* 4 p. ta
l-llll.l.ltUN a CENTS.
KVKMNO 7:1H Ud K:-.V
C-OMINO THriUil.AY—Ito BM .... l.-k. M 11:11: IM-I'.i. 11, "Oil.
VKR TWIST," Dickens' lamoas -tan-
NEW TELESCOPE IS
LARGEST IN WORLD
IkMM ii Canadian Gown-
not Obsanratory.
EXPECT NEW DISCOVERIES
Tbe Coterl* Clnb will meet January 4 at 8 p. m. at the I'nltarian
Church halt.
• * .
Th* I-o. Angcte. Herald says that
David A. Curry, th* picturesque Callfornlan. of Palo Alto Is drawing good
crowd* to bts lectures and picture*
on Yo**mlt* valley and th* hlgb
Sierras. I
. ••••
One or the most difficult tasks
ever undertaken by the Lasky Com-,
p.jir. was the securing of types for]
Its adaptation of "Oliver Twist,"!
wblh will be the Paramount picture
at the Varsity Tb*at*r tomorrow]
and Friday, with th* charming Ma- J
tie Doro as Oliver, supported by an
t. i-llent caat.
t • •
Th* seat sal* tor "Tbe Cay Gam**"
a high school Thespian Club production, will be thrown open Friday,
January ■"». at Weingartner'. drag
store. Ticket, to thc comedy will
•ell at 50 cent* apiece. In anticipation of a large crowd Business Manas-.-. K. Iiraunschwalgcr bas secured
the Armory for the evening of the
production Friday, Jsnusry 12. Admittance cards win also be sold by'
high srhool students.
• • •
With thc reopening of school §•▼«]
-oral new pupils have registered at]
the Palo Alio t'nlon High School.
Among the new students are Ethel
ami N'eal Templln. wbo bare entered
the freshman-A clas* They ball
from Rupert High School. Idaho.
Aldine Wellut.n ha> re-ruined the
Junior-it clsss. after an absence of
one semester during which time she
attended a prlrate .cbool tn Piedmont.
Hiff* Mirror Will Assist In Ftp-eating
Secrets of Ski..—OriBin of T.lsscop*
Wiled In Myst.r,—Polish Scientist
First to M.nt.on Petalblllty of M*flai-
f/lng Objects.
A taleacop* w*lghlng fl-ty-five tons
and having an observation tab* thlrty-
ooe feat loug recently has been Install-
ed at the gorernment astronomical ob*
aerratorr, Victoria, Canada. It la tb*
greatest Instrument uf lu kind tn tb*
world, and astronomer* expect to make
Important new Ji*merit-, by its aid.
Tb* giant tele-sco-** Is of tb* refi«ct-
log type and ba. a minor ln plac* of
a lens for contracting light raya at d
r»V*allng distant planets. This mirror
measures aeveoty-tbre* lncbe* and Is
twelr* lncbe* thick at tbe edg**.
neighing two and m quart** tous.
All of tb* p*n« of tb* tele-scope ara
Pn>r«cjor C D. Mart o: Palo Allo
left thl. morning ror N'ew York,
whir* he will attend a meeting ol «J«tricaUj operated, and tbe installa-
englneering *xp*rt.. U°tn °f **• JS™** l™™** " **""
, m # c*t* engineering problem.
__,_,,. | A Lost Ch.pt.r In History.
Mr*. J. D. Follm*r of Pair. Alto, ■„,* Utert iChl(.Tem<.Bt ,n J, ,d.
mother of R. W. Foltm«r i* In Grand Tanc*m*nt of astronomy calla to mind
Rapid.. Mich., taking trertment at that tb* origin of tb* t*l**cope Is a
th* Rurlraon Sanltarlnm. lost chapter ta history.
• • • Vltello, a Polish scientist wbo flour-
Mr. and Mrs. W. c. Werry re- »** ^JS? '?"**_* 'J* !!__!______
' ..... lying of object* when Mew*.] through
turned l«st *«nlng from Grass \*\* „ wtgaml , , „lhw of |I|H> wblle
l*y, where they attended tb* funeral u0fffr »_■_•->-, |„ -■*■-, „r tiig wortu tells
or a favorite uncle, a brtther of uf experiment, mndo wllb l.:,-.- in
Richard Werry ot Uttl city. mieb a way ibat onjr-ts men through
tbem would seem to !•*• magnified,
wblcb leave, little doubt tbat spectacles were actually In us* tu those
day*. .
Still otber author! tlce, notably IVter
Borrlllea In bis work "I* Vcr* T.-I
Dr. David Starr Jordan, chancellor
emerllu. of Stanford CniTer.lt>. U
the author of the leading ankle in
the December Snnaot, entitled "Wbat
of tb* Nation?" Tbe -.ob.lt I*.— eecopil invent.-ri' award eredll
"The Charge Against Democracy." Zacharlafa Jan. or Jansen and Hans
'Victory and Defeat." "The Soullns. I-apprry. twlti of whom In WOO were
War"- Indicate the high points oPWtlclane kuTI spectacle makers living
at Ulddleburg. Holland. It Is further
told buw about the year lfilO these two
entcri>ri.tng scientist., having Invented ttie ii-l.«. "in*, preeented one to
■ertalned th* Rank of Palo Alio *m- ^nre Maurtr* or Naaaan
ploye. at dinner New Year . day. aa Ualllei. who by reesou of lit* even
usual Th* wive* wet* also pii-*«nt. then worldwide eminent** was always
making the total nu miter at table the first to receive Inrnrmatlon of any
fourteen. Mr. Parkinson's first New development lu aclrntlflc research, no
sooner beard from a Oermsn savant
nf the new magnifying Instrument'
Doctor Jordan'a Brittle.
Mr and Mr.. G. R. Parkinson en-
OUR
Annual Clearance
SALE
Starts Wednesday December 27th
SPECIAL SALE PRICES ON ALL
Men's Suits. Overcoats, Hats, Caps
and Furnishings
Men's Suits From .... 11.95 up
Boys'Short Pant Suits - -' -. 3.50 Hats cut to - ' - v - 2.50 Hats cut to - - - - 1.15
Children's Hats Half Price
Christy, Rhodes & Co.
PHONE 200
Year's dinner to ihn employ it of the
bank required only one extra piste.
as he managed the batik at first with
onlv one assistant.
than he wt lo work lo Improve upon II
An Early 7-.... ,..,_..
The teles-col*** constructed by (inHid ;
ron.lsted of one entires object glass j
Mnniaulla Hall will ,..,-,,; lis tmI 0D# ,-oneav* eyeglass, which were
second semester January _*|h. Tu*.- placed at tbe extremities of a metal
daj Is scheduled for registration day lutte, the first »f these magnifying tbe '
and assignment of lessons, regular belght and Itreadttt of ol*Je*ii three
work beginning Wednesday January ,lm**- l**n °° h* ProdlK'*'1 * »*•*- -
i-'th Roys are received both as ___^_t^__\7^___t__!_l!!lS_
- tb
- tb
Ww:
V cfc
lo
ot
Writing Made E.iy.
Steadily tbe roads that lead to suc-
«_**. In literature ar* being made easier A good many people wbo would
Uke to write Action, but bare shrunk
frotn tbe labor of finding equivalent,
for "sal-l." will welcome a list of sub-
atttute. ror the necessary little word
published tn the Rmtou -Vrttur. There
are no less thsn ::**•: fslrly satisfactory
variation., frtuu "acceded" to "yowled-"
The prisoner at the tar uo longer
need say that b* I. Innocent. H.* can
bebtile It or bawl It or blurt It or
breatb* It. to n— but one letter of the
alphabet The vindictive district attorney can bark bis qoestltms. tb* Uw-
jer for tbe *l**fi ti-c ran boom bis "1-
jectlons. tbe .pert.ton m*y either l»rU-
Ue their Indignant protect or blubber
fjbelr symiwtby. Rut tlie merit of such n
t la not simply lhat It facilitates the
j-puitLgentent of dialogue. A thorough
udy of tbe ;■<■ .uhatttutcs for "said"
■wi;: easily .uggest the plot and the
chancterlxatlun that must prece_le dialogue. Tbe letter (* ln Itself Is a compendium of plot and a gallery of portraits. Given somebody who cachl-
Bales, another wbo . Im- Ll.-*. another
vbo coo*, another wbo croons, sn-
other wbo chslicncea, another wbo con-
fe**e* antl minor characten wbo cry.
croak, commune and cut In, and it ls
plain that we have a scenario almost
ready made.-New York Po*t.
Wend*efu| Carving.
Dr. Peter Ollrer, wbo tlvetl In Fng*
land daring the early part of tbe eighteenth century, tells of seeing a carved
cherry stone which would be a wonder
even In tbla age of floe tools and fine
. workmanship. Tbe . tooe was one
from a common cherry, aad u|ton it
. were carved the heads of \2l po!*-*,
kings, queens, etu|-«>rors, saints, etc.
Small as they must neres-mrll;' have
been. It Is announci-d on Uie a-nrtiarlti
of Profesaor Ollrer tbat with a pwd
-z*..i*-** tbe beds of the \*.*--. and kitigs
could readily If illstlngulsbetl from
thi«e of llie qnam and sa'nrs by their
miter, ami crown*. The gentleman
wh-. broticht thl. little wuodcr to I_ug-
land purchased tt in Prussia, allowing
tbe original owner £3.000 for hla treasure. Tblnk of U—PSjOOO for a cherry
P. A. tCi
-Advt.
Pianos tuned, phonographs te
paired 11. Schomberg tk Son. Ph. _.?.
about flve yean further along produc-
boarding and day pupils, either for ^ % ^^J wU|. ,Up JJ of ^
he high whool course, or the gram- nXtying object, altout thirty limes
mar crsrtes Full Information can b* ■ larger tbau they ap|war lo the unss-
had from W, A. Khedd. Telephone .luted eye.
Hut It was not until 1074 that Dr.
Hook* perfected the flnt relli-ctiui;
telescope In whlcb the spectrum wa.
perforated ao tbat object* could be
viewed by looking directly at them.
So through tbe year of ITT7 the tele
Miu Harker wishes to aonouncv: -x^ ta thti j,,,^. 0f tUrce*slve aa-
tbat chlldr*n of primary age and; uvnomera and optician, contained
those who are ready for primary, many change* and Improvements un*
work will be Instructed by means of til In 17t_3 William Herscbel liegait tbe
such Montes«orl apparatus as Is construction of a .|i*ctrum powerful In
practicable in combination wUh cbamcter and forty feet In total length
resulting from Mrs. dttfejffS^ -StUS
viewing the Imsge through lho system
of eyeglasses. Wltb tbls telcacope.
completed ki ITM), object* were magnified G.S00 time., and on tbe nlgbt
nelfs yeara of experience In primary work. 12-30-2t~Advt.
Dancing—Mis. Carrie Lewis' ad
vanced rltu for children Thursday ttier lt wa. BnUbed Hencbel disc**-
January 4: beginner* Tuertay. Jan-*1** «*• •trlh «I«»»« of tD* *>**«
nary », 3: SO. Fraiemity Annex. 8alorn-
Adults' evening class on application c. .« nu nt c QUI I *-C rAQTI P
Studio. 73U Cowper at Phone 71 OM. FLAG 0N 0LE BULL b LAb ,Lt*
12.-_t0._t—l_dvt. aurt mnd 8tripM 0v#r old NocwSQian
* • * * Colony,
C.stllleja Scbool reopens January Tbe ruins of Ole Hull cotle. ln the
« at the regular hour The second Kettle Creek country r>e*r Coudera-
port. Pa., will hereafter fly the stars
a _td .tripe*.
Ur. and Mrs. W. F. Un* and Mr.
and Mrs. Jame* Sulllvsn or Genesee.
Pa., msde an offer of a Urge flag each
year. Tbey bsT* already given Henry
• ,*.• " Oleeon. wbo has charge of tbe castle
Madame H. Foreman Kmricb *x- proiterty, a flag which will float from a
cmpltfies and -tnachea lb* wonderful tall flagpole U> bl erected
term begins January 22. New pupils msy enter at any time. Appointment, may be mad* with th* principal. Phon* ItOR.
12-2»-7l—Advi.
"BROTHER JONATHAN."
Car-Mr ot th. Patriet Who Qav. Us
This Lasting Title.
Jonathan Trumbull, tb* original
"Brother Jonathan," was born on OcL
12, 1710, In lacbanoo. Conn. He was
graduated from Harvard college In
177., preached a few years, then stud
led law, and at tb* ag* of twenty-
three ye*r. b* waa a member of tbe
assembly. Ue was chosen lieutenant
governor In 1700 and became ex officio
chief Justice of tbe superior coorL la
170N be refused to lake th* oath en
Joined on ■■■■'■-■■;■■ ot tbe crown, and In
tbe following year be was chosen governor. He wa. tbe only colonial governor who ea-jiouscd tbe cause of thc
peopl* lu tbelr struggle for Justice and
Independence.
In tbe abaence at congrcsa of tb*
Adamses aud Hancock from New England Trumbull was considered tb*
Whig leader In that region, and Waab.
Ington always placed Implicit reliance
upon his pstrlotlsm and energy. Wnsn
Waahlngton look charge of th* Continental army at Cambridge, Mass., lit
found It In want of ammunition aud
other supplies, snd this want continued more or less for months. Trumbull wa. then governor of ConnectlcoL
On on* occasion at a council of war,
wbeo there M*med to b* no way to
make provision against an attack by
tb* enemy. Washington said. "We
must consult llrotber Jonathan on Ihla
aubject** He did so, and the governor
was succeaaful In supplying msny of
tbe needs of the army.
When tb* army was afterward
spread over tbe country and difficulties aros* It wa. a common saying
among tbe officers as a byword, "We
ma.t roosult Rrotbcr Jonathan." TW
origin of tbls ['hr.se waa *v«ntnally
l-"t -sight of. and "llrotber Jonathan"
becam* a tiiie of our nationality. Ilk*
"John Hall" of England. Trumbull
died In Lebanon, Conn., oo Aug. 17,
IT--'.
Tbe castl* ruin, .(snd a reminder of
tbe Norwegian cnloolsts wbo, nnder
tbe leadership of Ole Ball. Norwegian
muslciaa-patrtot, came to Potter county Id 1632. "We are to found a n*w
Norway consectated to liberty. Up-
. ■» _ fixed with Independence end (irotectcd
by America's mlgbtr fug." said Ole
It VYeuIdn't Souwl W*IL "n" In an addre.*. Cboo*lng Potter
An Engllsb north country p*p*r county for bts colontaatloo scheme, be
art ot dancing as the
health, happiness, grace of mind and
body. Studio room 12. Madlson-
Thoits building. Phone 3lt.
11-11-tf—Advt.
. . ImpMiinleua Irritation.
"You *my the couple quarreled on
tbelr honeymoon7"
"Yes. You aee, they hadn't mnch to
start wltb, and tne honeymoon bad
got to It. last quarter." — Baltimore
American.
purchased from a Philadelphia land
owner for *10,000 11.144 acree of land
at tbe beadwaten of Kettiu creek, in
wbat U now Abbott and Steward*on
township*, laying oat the villages of
Oleona. New Norway. New Bergeu
and Wn!inu.
frown, upon the known jmbltlou of
tbe mayor of Its town to be m.de .
knight for his distinguished services In
receiving royalty and narrates fur tbe
benefit of tbe aspirant thl. anecdote.
When Adam Black, the Edinburgh pub
Usher, w*_ aounded on th* subject of
receiving knighthood be said: "Nae Turk Government Aide Wmrmmem.
nee; it wadna dee. Ton •**,* b« a-l-; Tbe Turkish government has made a
ed. "if a boy cam' Into ma shop and special appropriation of 11,060,000 to
aald. 'A hapetb o' slate pencil. Sir b* osed for tb* pnrcbss* of seed grains
Adam.* Jt wadna sound wceL" tor needy TurkUb f.rmrn to the Turk-
\ tih dominions.
Davies Auto
Repair Co*
1002 SUte Highway
REDWOOD CITY
I't.one Redwood 407
Expert auto mechanics. AH
kinds of engine, pump or machinery repair work a specialty
LA PEIRE & SON
GROCERS
821 Ramona Street
Phone 29
Palo Alto Market
Lauridaon ft Sonnicksor.
i-iii.m: SSI
S-M lnl.rr.llj A.M..
LOOK FOB
THE GOLD HEAD
OX III R WAOONH
"Safety First"
HUN
"Highest Class Work"
The F. THOMAS Parisian
Dyeing and Cleaning Works
nu ai-ma man.
PHON. 117
Why Not Start the New Year
Right by patronizing
Fortune's Taxi Service
Phone 19
Day and Night Servic
