3,845 research outputs found

    BIODEGRADATION OF CYANIDE COMPOUNDS BY PSEUDOMONAS-FLUORESCENS IMMOBILIZED ON ZEOLITE

    No full text
    A cyanide-degrading bacteria, Pseudomonas fluorescens NCIB 11764, was used for the treatment of wastewater containing cyanide. After successive subculture for 2 months in cyanide-containing medium, cultures were fully adapted to cyanide and able to grow in a medium containing up to 260 mg l(-1) of cyanide. By fed-batch culture supplying only cyanide, it was confirmed that cyanide was the limiting nutrient as a nitrogen source. Ammonia was found to inhibit cyanide degradation, and the rate of degradation was halved when the ammonia concentration was higher than 1 mM. In a 15-l jar fermenter, 26 mg l(-1) of cyanide was consumed almost completely within 48 h and the final yield of growth on cyanide was calculated as 4.21 mg dry cell wt mg(-1) CN. Cells of P. fluorescens were immobilized on zeolite and applied to a modified activated sludge-type reactor to mimic the actual activated sludge system of a coke-plant wastewater treatment system. In this immobilized system operated batchwise, 26 mg l(-1) of cyanide was degraded completely by 8 g l(-1) of immobilized particles within 10 h. The same reactor system was operated in a continuous mode, and the cyanide concentration of the outlet reached the stationary-state level of less than 0.1 mg l(-1) within 2 days at 0.041 h(-1) of dilution rate and within 3 days at 0.082 h(-1) of dilution rate, respectively

    OPTIMIZATION OF 2-STAGE CONTINUOUS-CULTURE SYSTEM FOR PRODUCTION OF POLY-BETA-HYDROXYBUTYRATE

    No full text
    The optimization of poly-beta-hydroxybutyrate (PHB) fermentation process is essential to obtain a high PHB productivity. An optimization of two-stage continuous culture system by complex method for PHB production is presented. PHB production rate of the system obtained in the present study was chosen as the objective function. The PHB production rate of the system was higher than that of the phasewise optimization method which maximizes the growth rate of residual biomass in the first stage and the PHB production rate in the second stage. When the inlet glucose concentration in the first stage increased, PHB content and yield also increased. When the fermenter volume ratio (V-2/V-1) was 0.5, maximum PHB productivity in the second stage was 2.86 g/L . h, which is highest compared with the reported value on PHB
    corecore