734 research outputs found

    Chitosan hybrid nanoparticles as a theranostic platform for targeted doxorubicin/VEGF shRNA co-delivery and dual-modality fluorescence imaging

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    The current strategies for drug/gene and multimodal imaging probes integrated into a single nanoparticle have some limitations still. Here, multifunctional chitosan hybrid nanoparticles (denoted as FA–CS–FITC(DOX/C-dots)/VEGF shRNA) containing folic acid (FA), fluorescein isothiocyanate (FITC) and doxorubicin (DOX)/carbon quantum dots (C-dots)/VEGF shRNA were fabricated as a targeted drug/gene co-delivery nanovector for potential cancer therapy and fluorescence imaging. The self-assembled FA–CS–FITC(DOX/C-dots)/VEGF shRNA nanocomplexes exhibited a desirable and homogenous particle size (154 24 nm), moderate positive charges (23.2 1.8 mV) and superior stability. The nanocomplexes without noteworthy cytotoxicity are capable of delivering VEGF shRNA into human cervical cancer HeLa cells with high efficiency while effectively protecting shRNA from degradation by exogenous DNase I and nucleases. The release behavior of DOX exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release at both pH 7.4 and pH 4.5, and also presented a pH-triggered release profile. Confocal microscopy analysis confirmed that both FA-targeted function and FA-enhanced buffering capacity induced high transfection, specific cellular uptake and efficient intracellular delivery of FA–CS–FITC(DOX/C-dots)/VEGF shRNA nanocomplexes in folate receptor-overexpressed HeLa cells. Transfected HeLa cells exhibited significantly decreased VEGF expression, inhibited cell proliferation, and increased cell apoptosis, which led to synergistic antitumor activities. Furthermore, the nanocomplexes demonstrated excellent dual fluorescence cellular imaging at a modest concentration. This work indicates that the integrated theranostic design of FA–CS–FITC(DOX/C-dots)/ VEGF shRNA nanocomplexes potentially allows for the image-guided and target-specific treatment of cancer.</p

    Performance improvement of coal-fired power generation system integrating solar to preheat feedwater and reheated steam

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    Solar aided power generation (SAPG) technology has been proven as an efficient way to use solar energy for power generation purpose. In a SAPG plant, a solar field consisting of parabolic solar collectors is normally used to supply the solar heat to preheat feedwater and replace the high-pressure extraction steam. However, the outlet temperature of reheated steam decreased with the integration of solar thermal which sacrificed the system performance. As an improvement, the second solar field was proposed to preheat the reheated steam to maintain its outlet temperature and the average temperature of regenerative Rankine cycle before or after solar integration. Through a case study using A 600MW supercritical SAPG plant operating in fuel-saving Using solar to preheat feedwater and reheated steam could improve overall cycle efficiency and solar-to-electricity efficiency by 1.91% and 6.01%, respectively. The solar share was expanded from 9.53% to 17.26%. The electricity generated per solar aperture area under the design condition was 187.96 W/m², which was 26.91% higher than that of the system without the second solar field.Junjie Wu, Hongjuan Hou, Eric Hu, Yongping Yan

    Evaluation of solar aided biomass power generation systems with parabolic trough field

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    Solar and biomass are both renewable energy resources. Using biomass as fuel is becoming more and more attractive after governments increase the tariff for the electricity from the renewable sources. However the costs of power from a biomass power generation plant depend greatly on the availability and quality of the biomass resource. The commercialization of solar alone thermal power generation is hindered by its high initial investment and low thermal efficiency. In this paper, a concept of integrating solar into a biomass power generation system is put forward. In the system the oil heated by a parabolic trough solar field is used to replace the extraction steam to preheat the feed water (entering a biomass boiler) and the previous extraction steam thus saved can continue to do work in the lower stages of turbine. The performance of the hybrid system with different replacements is analyzed and compared through two typical solar aided biomass generating units. The results show that the integration not only reduces the consumption of biomass fuel (at the same generation capacity) but is also proved to be an efficient way to convert solar thermal energy into power. The results also show that with the same solar aperture area, the higher the grade of the replaced extraction steam, the better the thermal performance and economy.Hou, HongJuan; Yang, YongPing; Hu, Eric; Song, JiFeng; Dong, ChangQing and Mao, Jia

    Molecular cloning and functional characterization of a short peptidoglycan recognition protein (HcPGRPS1) from the freshwater mussel, Hyriopsis cumingi

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    Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. In the present study, a short-form PGRP, designated as HcPGRPS1 was identified from freshwater mussel Hyriopsis cumingi. The deduced amino acid sequence of HcPGRPS1 is composed of 235 residues which contains a conserved PGRP domain at the C-terminus. Sequence analysis showed that HcPGRPS1 shared high identities with other known PGRPs. The mRNA of HcPGRPS1 is constitutively expressed in a wide range of all tested tissues, with highest expression level in hepatopancreas, and its expression in tissues (gonad, nephridium, gill and foot) was up-regulated significantly after LPS or PGN stimulation (P<0.05). The recombinant protein of HcPGRPS1 exhibited binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and DAP-PGN from Bacillus subtills. Furthermore, recombinant HcPGRPS1 displayed strong antibacterial activity to both Gram-negative bacteria Escherichia coli, Aeromonas hydrophila, Aeromonas sobria and Gram-positive bacteria S. aureus in the presence of Zn2+. These results suggested that HcPGRPS1 plays a multifunctional role in the defense and protection mechanisms of mussel innate immunity against infections. (C) 2013 Elsevier Ltd. All rights reserved.Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. In the present study, a short-form PGRP, designated as HcPGRPS1 was identified from freshwater mussel Hyriopsis cumingi. The deduced amino acid sequence of HcPGRPS1 is composed of 235 residues which contains a conserved PGRP domain at the C-terminus. Sequence analysis showed that HcPGRPS1 shared high identities with other known PGRPs. The mRNA of HcPGRPS1 is constitutively expressed in a wide range of all tested tissues, with highest expression level in hepatopancreas, and its expression in tissues (gonad, nephridium, gill and foot) was up-regulated significantly after LPS or PGN stimulation (P<0.05). The recombinant protein of HcPGRPS1 exhibited binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and DAP-PGN from Bacillus subtills. Furthermore, recombinant HcPGRPS1 displayed strong antibacterial activity to both Gram-negative bacteria Escherichia coli, Aeromonas hydrophila, Aeromonas sobria and Gram-positive bacteria S. aureus in the presence of Zn2+. These results suggested that HcPGRPS1 plays a multifunctional role in the defense and protection mechanisms of mussel innate immunity against infections. (C) 2013 Elsevier Ltd. All rights reserved

    Critical roles of edge turbulent transport in the formation of high-field-side high-density front and density limit disruption in J-TEXT tokamak

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    This article presents an in-depth study of the sequence of events leading to density limit disruption in J-TEXT tokamak plasmas, with an emphasis on boundary turbulent transport and the high-field-side high-density (HFSHD) front. These phenomena have been extensively investigated using a Langmuir probe and Polarimeter–interferometer diagnostics. The research reveals a consistent pattern of events as the plasma density ramps up: the collapse of the sheared radial electric field, the enhancement of the boundary quasi-coherent turbulence (50 ∼ 80 kHz), the increase in boundary particle transport induced by this turbulence, edge cooling and the emergence of the HFSHD front. These phenomena occur once the plasma density exceeds a critical value. Importantly, by exploring plasmas with varying edge safety factor (qa), it is revealed that the density thresholds for these phenomena are all inversely proportional to qa. The findings offer valuable insights into the mechanisms underlying density limit disruptions in tokamak plasmas, suggesting that the enhancement of edge turbulent transport plays a crucial role in the edge cooling and triggering the HFSHD front. For the first time, a strong link between the edge turbulent transport and the HFSHD front has been observed. In addition, the evolution of the boundary electron temperature across various plasmas can further validate the link between the edge turbulent transport and the HFSHD formation

    miR136 regulates proliferation and differentiation of Small Tail Han sheep preadipocytes

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    The data that support the findings of this study are available from the corresponding author upon reasonable request

    Study on the Intercultural Communication of Traditional Chinese Culture

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    In the era of globalization and the rapid growth of we-media, intercultural communication plays a crucial role in promoting traditional Chinese culture on an international scale. China has been actively engaged in advancing intercultural communication as a means to enhance its cultural soft power, fostering the high-quality development of its culture and contributing to the realization of the Chinese nation's rejuvenation. This study focuses on the intercultural communication of traditional Chinese culture, using Li Ziqi's vlogs as a case study, in order to provide updated insights for the promotion of China's traditional culture in diverse cultural contexts, effectively conveying a long-standing image of Chinese civilization. Through the analysis of themes and categorization of Li Ziqi's short videos, the study defines the cultural symbolism conveyed through these videos, explains the factors that contribute to their success, and draws conclusions that can enlighten the international dissemination of traditional Chinese culture. Furthermore, it serves as a model case for practicing intercultural communication of traditional Chinese culture. The study's findings emphasize the importance for Chinese cultural promotion to seize the opportunities presented by we-media development, accurately targeting the needs of overseas audiences to enhance the effectiveness of cultural communication, and facilitating a deeper understanding of the rich connotations of traditional Chinese culture among foreign audience

    The Strategies of Advancing the Cooperation Satisfaction among Enterprises Based on Low Carbon Supply Chain Management

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    AbstractWith the end of United Nations Convention on Climate Change Meeting which was hold in Bonn and Copenhagen,the world has entered into a low carbon economy which bring the concept of low carbon supply chain management.In order to comply with the changes of the domestic and international situation,enterprises are supposed to adopt low carbon supply chain management to raise the competitiveness. However,it is a systems engineering to carry out the low carbon supply chain management in which the good cooperation of each enterprises in the supply chain is required.The paper proposes some strategies on how to advance cooperation satisfaction among enterprises based on low carbon supply chain management in order to enhance the competence of the whole supply chain
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