651 research outputs found
Transcriptome analysis of Hydra magnipapillata exposed to pharmaceuticals
No full textA large number of pharmaceuticals for human and veterinary practices have been continually released in aquatic environments. These pharmaceuticals potentially induce various adverse effects in aquatic organisms. This study investigated lethal or sublethal concentrations, morphological changes and physiological effects of fluoxetine [FLX], diclofenac [DIC], ibuprofen [IBU], naproxen [NAP] and acetaminophen [APAP] in H. magnipapillata. Median lethal concentrations (LC50) of these pharmaceuticals for 24, 48 and 72 h were 3.678, 3.082 and 2.901 mg/L for FLX 13.017, 8.147, 7.045 mg/L for DIC 57.847, 42.191, 32.501 mg/L for IBU and 51.999, 44.935, 42.500 mg/L for NAP, respectively. Median inhibitory concentrations (IC50) of APAP were 231.335 and 487.901 mg/L for 72 and 96 h, respectively. Morphological analysis showed that FLX, IBU, DIC and NAP induced retraction on tentacles and body column with slight variations. However, APAP did not induce any morphological changes, but inhibited head and tentacles regeneration. Transcriptome analysis showed that FLX induced oxidative stress, DNA damage, growth and developmental effects, and impaired gametogenesis DIC induced DNA damage, endoplasmic reticulum (ER) stress, protein degradation and inflammation IBU induced ER-stress and oxidative stress NAP induced ER-stress, growth and developmental effects and inflammation and APAP induced oxidative stress, DNA damage and impaire1
Differentially expressed genes in Naproxen exposed Hydra
No full textThe extensive use in humans and animals of nonsteroidal anti-inflammatory drugs (NSAIDs) increases their possible impact on aquatic organisms. In the present study, we investigated acute toxicity, morphological responses, and potential physiological and metabolic impacts of naproxen exposure on Hydra magnipapillata. The median lethal concentrations (LC50) of naproxen in H. magnipapillata were 52.0 mg/L, 44.9 mg/L, and 42.5 mg/L after exposure for 24, 48, and 72 h, respectively. Morphological observation of the exposed Hydra showed that 40 mg/L naproxen stimulated the contraction of body column and tentacles after 24 h. A KEGG pathway analysis of the genes differentially expressed in the Hydra after exposure tonaproxen for 6, 24, or 48 h demonstrated various cellular and metabolic effects, including protein processing in the endoplasmic reticulum, Wnt signaling, and tryptophan metabolism. These results suggest that exposure to naproxen affects the genetic material, inflammatory processes, and metabolic processes of aquatic organisms.1
TRANSCRIPTOMIC PROFILING OF HYDRA AFTER EXPOSURE TO NAPROXEN
No full textThe extensive use in humans and animals of nonsteroidal anti-inflammatory drugs (NSAIDs) increases their possible impact on aquatic organisms. In the present study, we investigated acute toxicity, morphological responses, and potential physiological and metabolic impacts of naproxen exposure on Hydra magnipapillata. The median lethal concentrations (LC50) of naproxen in H. magnipapillata were 52.0 mg/L, 44.9 mg/L, and 42.5 mg/L after exposure for 24, 48, and 72 h, respectively. Morphological observation of the exposed Hydra showed that 40 mg/L naproxen stimulated the contraction of body column and tentacles after 24 h. A KEGG pathway analysis of the genes differentially expressed in the Hydra after exposure tonaproxen for 6, 24, or 48 h demonstrated various cellular and metabolic effects, including protein processing in the endoplasmic reticulum, Wnt signaling, and tryptophan metabolism. These results suggest that exposure to naproxen affects the genetic material, inflammatory processes, and metabolic processes of aquatic organisms.1
Acetaminophen responsive genes in hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same
No full text본 발명은 아세트아미노펜(Acetaminophen) 노출에 대응하는 히드라(Hydra magnipapillata) 유전자 및 이
를 이용하여 수생태계 환경오염을 진단하는 방법에 관한 것으로, 히드라에 아세트아미노펜을 노출시킨 후
변화되는 유전자를 분석한 결과, 5000 ㎍/L의 아세트아미노펜에 12시간 노출군에서 32종, 24시간 노출군에
서 66종, 48시간 노출군에서 37종, 72시간 노출군에서 84종의 유전자들이 발현량이 변화되는 것을 확인하
였고, 상기 차등발현 유전자들 중 생체 내 생리 및 대사와 연관되는 것으로 예상되는 25종의 유전자들을
선별하였으며, 선별된 유전자들의 기능 분석을 통해 아세트아미노펜의 노출이 히드라의 어떤 생물학적 기
능에 영향을 미치는가를 예측할 수 있는 바, 상기 25종의 유전자들은 아세트아미노펜 노출을 확인할 수 있
는 바이오마커로 유용하게 활용할 수 있다
Fluoxetin responsive genes in hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same
No full text본 발명은 플루옥세틴(fluoxetin) 노출에 대응하는 히드라(Hydra magnipapillata) 유전자 및 이를 이용하
여 수생태계 환경오염을 진단하는 방법에 관한 것으로, 히드라에 플루옥세틴을 노출시킨 후 변화되는 유전
자를 분석한 결과, 30 ㎍/L의 플루옥세틴에 12시간 노출군에서 1종, 24시간 노출군에서 1종, 48시간 노출
군에서 21종, 72시간 노출군에서 18종의 유전자들이 발현량이 변화되는 것을 확인하였고, 300 ㎍/L의 플루
옥세틴에 12시간 노출군에서 3종, 24시간 노출군에서 1종, 48시간 노출군에서 3종, 72시간 노출군에서 3종
의 유전자들이 발현량이 변화되는 것을 확인하여, 상기 유전자들의 기능 분석을 통해 플루옥세틴의 노출
이 히드라의 어떤 생물학적 기능에 영향을 미치는가를 예측할 수 있으며, 상기 27종의 유전자들은 플루옥
세틴 노출을 확인할 수 있는 바이오마커로 유용하게 활용할 수 있다
Microbiota changes in diseased Hydra magnipapillata
No full textThe bacterial community associated with apparently healthy and diseased Hydra magnipapillata was investigated by pyrosequencing analysis of bacterial 16S rRNA. Proteobacteria and Bacteroidetes were the main phyla associated with the apparently healthy specimens. In diseased Hydra, Chlorobi, Cyanobacteria, Planctomycetes and Actinobacteria were also present in addition to Proteobacteria and Bacteroidetes. The diseased specimens were associated with greater abundance of Proteobacteria, Betaproteobacteria, Burkholderiales and Comamonadaceae. The major taxa contained potentially pathogenic bacteria that may influence health of H. magnipapillata. Species diversity and community composition significantly differed between healthy and diseased H. magnipapillata. © 2017, Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media B.V., part of Springer Nature.33scopu
A simple and rapid method for toxicity evaluation of zinc oxide nanoparticle (ZnO NPs) in benthic animal Hydra magnipapillata
No full textToxicity evaluation is necessary to investigate the possible risk of chemical or pollutants newly produced such as nanoparticles in the environments. The assessment should apply a method that is effective to determine the toxic concentration and the exposure time of the pollutants in an animal model. This study described three main stages including determining the median lethal concentrations (LC 50 ) with Probit program and detecting toxic effects of ZnO NPs in morphology and regeneration observed by the changes in morphology of Hydra magnipapillata (H. magnipapillata). We also provide a strategy for culturing hydra in laboratory condition to use the animal for the experiment. The exposure to ZnO NPs led to the abnormality in regeneration such as formation of extraordinary number of tentacles and bifurcated tips in tentacles and the toxic effects in morphology appeared the clubbing tentacle, slender body, and retracting body column and tentacles by the exposure time. The method described here is simple and useful to evaluate the toxic effects of ZnO NPs using morphological characters in H. magnipapillata and could suggest the concentration and the exposure time for further investigations on cellular and molecular responses of the animal after exposure to other nanoparticles. • A simple method to evaluate the toxic effects of ZnO NPs using morphological characters of H. magnipapillata and other hydra species. • A rapid method to evaluate the toxic effects of ZnO NPs and other nanoparticles in H. magnipapillata. © 2019 The Authors33scopu
Acute toxic effects of zinc oxide nanoparticles on Hydra magnipapillata
No full textZinc oxide nanoparticles (ZnO NPs) are increasingly used in various products as coating and additive materials for household goods, personal-care products, and drug delivery systems. Because of their broad applications, the potential risks to nontarget organisms associated with their input into aquatic environments have generated much concern. We investigated the acute toxicity, morphological responses, and potential impact on physiology and metabolism in polyps exposed to spherical ZnO NPs of either 20 nm (ZnO NP20) or 100 nm (ZnO NP100). The median lethal concentrations (LC50) of ZnO NP20 were 55.3, 8.7, and 7.0 mu g/mL after exposure for 48, 72, and 96 h, respectively; and those of ZnO NP100 were 262.0, 14.9, and 9.9 mu g/mL, respectively. The morphological responses of the hydra polyps to a range of ZnO NP concentrations suggest that ZnO NPs may negatively affect neurotransmission in Hydra. ZnO NPs may also induce abnormal regeneration in the polyps by affecting the expression of several genes related to the Wnt signaling pathway. The presence of ZnO NP20 in the hydra tissue was confirmed with electron microscopy. A Gene Ontology analysis of the genes differentially expressed in hydra polyps after exposure to ZnO NP20 for 12 or 24 h revealed changes in various processes, including cellular and metabolic process, stress response, developmental process, and signaling. A KEGG pathway analysis of hydra polyps after exposure of ZnO NP20 or ZnO NP100 for 12 or 24 h demonstrated various changes, including in the DNA replication and repair, endocytosis, lysosomes, Wnt signaling, and natural killer-cell-mediated cytotoxicity pathways, suggesting the mechanisms that maintain cellular homeostasis in response to ZnO NPs. Progesterone mediated oocyte maturation was also affected by the ZnO NPs nanoparticles, suggesting that they are potential endocrine disruptors. This study should increase our concern regarding the dispersal of ZnO NPs in aquatic environments.11sciescopu
Ross-Ade
No full textDave Ross (1871-1943) and George Ade (1866-1944) were trustees, distinguished alumni and benefactors of Purdue University. Their friendship began in 1922 and led to their giving land and money for the 1924 construction of Ross-Ade Stadium, now a 70,000 seat athletic landmark on the West Lafayette campus. Their life stories date to 1883 Purdue and involve their separate student experiences and eventual fame. Their lives crossed paths with U.S. First Lady Eleanor Roosevelt, Henry Ford, Amelia Earhart, and Will Rogers among others. Gifts or ideas from Ross or Ade led to creation of the Purdue Research Foundation, Purdue Airport, Ross Hills Park, and Ross Engineering Camp. They helped Purdue Theater, the Harlequin Club and more. Ade, renowned author and playwright, did butt heads with Purdue administrators at times long ago, but remains a revered figure. Ross's ingenious mechanical inventions of gears still steer millions of motorized vehicles, boats, tractors, even golf carts the world over
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