1,721,056 research outputs found
Specific PCR detection of Phytophthora megasperma using the intergenic spacer region of ribosomal DNA
No full textRibosomal RNA genes (rDNA) possess suitable characteristics for the detection of pathogens at the species level. They occur in multiple copies arranged in tandem repeats with each repeat consisting of the 18S Small SubUnit (SSU), the 5.8S, and the 28S Large SubUnit (LSU) genes, separated by internal spacers (ITS). ITS have proven particularly useful for separation of Phytophthorae at the species or genus level, although P. megasperma still remain one of the most problematic species in the genus. The utility of the region between the LSU and SSU genes, known as the intergenic spacer (IGS), has not been fully exploited for diagnostic purposes in Phytophthora. Thus, to develop a specific molecular tool, the complete sequence of the IGS region in P. megasperma was determined. Primer pairs were designed for amplification of the region spanning a portion of the LSU, the IGS, and the 5' end of SSU. The tandem repeated unit was 5,259 bp long appearing to be the shortest rDNA cluster described so far among the available sequences. A pair of oligonucleotide primers (IGph6 and IGph5), designed on the P. megasperma IGS sequence, were evaluated for specificity and absence of cross reactivity, by using a wide range of Phytophthora species and other fungi, commonly occurring in the soil. The resulting amplicon was of the expected size (176 bp), thus allowing the specific identification of all P. megasperma isolates, but not of those known as the AC form (isolate SCRP448)
EFFECT OF BIO-CONTROL AGENTS USING A COMPOST-BASED ORGANIC MEDIUM ON CITRUS PLANT GROWTH AND ON PHYTOPHTHORA CITRUS ROOT ROT
No full textEffect of bacterial and fungal antagonists on the growth of olive plantlets and on the inoculum density of Verticillium dahliae microsclerotia
No full textQuantitative detection of Phytophthora nicotianae zoospores and chlamydospores by real-time Scorpion PCR
No full textSUPPRESSION OF SOIL BORNE DISEASES OF CITRUS AND OLIVE PLANTATIONS USING ORGANIC MEDIA AMENDED WITH BIOCONTROL AGENTS
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SEASONAL VARIATION IN PHYTOPHTHORA SPP. IN CITRUS NURSERIES IN SOUTHERN ITALY: PRELIMINARY RESULTS
No full textMicrobial antagonists and compost-based growing media affect the growth of olive plantlets and the inoculum density of verticillium dahliae microsclerotia
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