1,205 research outputs found
Measuring accuracy of delivery quantities from ZF Aftermarket Frydlant
No full textBakalářská práce na téma Statistické měření přesnosti dodávek výrobků od ZF Aftermarket Frýdlant je zaměřena na aplikaci statistických metod, díky kterým je možné získat potřebné informace o tom, jak si pobočka ZF Aftermarket Frýdlant stojí v roli dodavatele.V teoretické části bakalářské práce jsou uvedeny poznatky týkající se dodavatelsko-odběratelských vztahů, logistiky a statistických analýz. V praktické části je popsána práce s vnitřními systémy společnosti ZF Aftermarket Frýdlant a dále s předplacenými programy od společnosti Microsoft jako jsou MS Word, MS Excel a MS Access. Tyto programy napomáhají vytvoření databáze, která bude sloužit jako týdenní report pro Supply Chain Managera a vedení společnosti pobočky ZF Aftermarket Frýdlant.Cílem práce je vytvoření sebereflexního zhodnocení pobočky ZF Aftermarket Frýdlant v roli dodavatele.The bachelor thesis Measuring accuracy of delivery quantities from ZF Aftermarket Frydlant is focused on the application of statistical methods, which enables us to obtain the necessary information on how the ZF Aftermarket Frýdlant branch proves itself to be a supplier.The theoretical part of the bachelor thesis presents current knowledge of supplier-buyer relationships, logistics and statistical analyses. In the practical part of this work, the author works with ZF Aftermarket Frýdlant internal systems, as well as prepaid Microsoft applications such as MS Word, MS Excel and MS Access. These applications help to create the database which would be used as a weekly report for the RMG Manager and the management of the branch ZF Aftermarket Frýdlant. The main aim of this thesis is to create a self-reflective evaluation of the branch ZF Aftermarket Frýdlant as a supplier
Ethanol ablation of hepatocellular carcinoma Up to 5.0 cm by using a multipronged injection needle with high-dose strategy
No full textPURPOSE: To investigate whether ethanol ablation by using a multipronged needle delivery system (multipronged ethanol ablation) could eradicate hepatocellular carcinoma (HCC) up to 5.0 cm in diameter with a single-session high-dose strategy.
MATERIALS AND METHODS: The hospital ethics committee approved the prospective study, and each patient provided written informed consent. One hundred forty-one patients (125 men, 16 women; mean age, 53 years; range, 27-76 years) with 164 primary or recurrent HCC ranging from 1.3 to 5.0 cm in diameter (mean, 2.9 cm +/- 0.9) were treated with high-dose multipronged ethanol ablation. Patients were unsuitable for surgery, declined surgery and radiofrequency ablation, or had tumors located at unfavorable sites. Primary technique effectiveness (PTE) (complete ablation within two sessions), local tumor progression (LTP), and complications after the treatment were observed. Twenty risk factors of local effectiveness and complications were analyzed by means of univariate and multivariate analysis.
RESULTS: Mean number of treatment sessions was 1.1. The mean volume of ethanol per tumor was 31 mL (range, 8-68 mL). PTE was achieved in 134 (95%) of 141 patients and was significantly associated with tumor pattern (capsulated vs noncapsulated, P = .018). After a mean follow-up period of 25 months, LTP was observed in 16 (12%) of 134 patients, and in nine (56%) patients, LTP occurred in tumors 3.1-5.0 cm in diameter. Alanine aminotransferase level (P = .023) was the independent risk factor for LTP. Three (2%) of 141 patients had major complications.
CONCLUSION: Multipronged ethanol ablation with a high-dose strategy can be used to treat HCC up to 5.0 cm in diameter effectively and safely, often in a single sessio
Half-ZF beamforming scheme for downlink two-user multiple input single output-based non-orthogonal multiple access systems
No full textThe authors investigate the beamforming (BF) problem to minimise the power consumption for the downlink 2-user multiple input single output-based non-orthogonal multiple access system. Inspired from the conventional zero-forcing (ZF) scheme, they propose the Half-ZF scheme which only mitigates the interference introduced by the signal of the cell-interior user through the ZF method. Meanwhile, the interference introduced by the signal of the cell-edge user is removed through the SIC technology. We derive the closed-form expression for the BF vectors of the two users under the Half-ZF scheme. The optimal power consumption for the Half-ZF scheme is analysed and the corresponding solution of the BF factors are also obtained. Compared with the conventional ZF scheme, both the Half-ZF scheme and the existing quasi-degradation (QD) scheme can achieve lower power consumption than the ZF scheme. However, the Half-ZF scheme can achieve this advantage under more relaxed conditions. Consequently, the Half-ZF scheme consumes lower power than the QD scheme when combined with the ZF scheme in practical systems.SCI(E)ARTICLE101633-16401
Next-Generation Sequencing: Application in Liver Cancer—Past, Present and Future?
No full textHepatocellular Carcinoma (HCC) is the third most deadly malignancy worldwide characterized by phenotypic and molecular heterogeneity. In the past two decades, advances in genomic analyses have formed a comprehensive understanding of different underlying pathobiological layers resulting in hepatocarcinogenesis. More recently, improvements of sophisticated next-generation sequencing (NGS) technologies have enabled complete and cost-efficient analyses of cancer genomes at a single nucleotide resolution and advanced into valuable tools in translational medicine. Although the use of NGS in human liver cancer is still in its infancy, great promise rests in the systematic integration of different molecular analyses obtained by these methodologies, i.e., genomics, transcriptomics and epigenomics. This strategy is likely to be helpful in identifying relevant and recurrent pathophysiological hallmarks thereby elucidating our limited understanding of liver cancer. Beside tumor heterogeneity, progress in translational oncology is challenged by the amount of biological information and considerable “noise” in the data obtained from different NGS platforms. Nevertheless, the following review aims to provide an overview of the current status of next-generation approaches in liver cancer, and outline the prospects of these technologies in diagnosis, patient classification, and prediction of outcome. Further, the potential of NGS to identify novel applications for concept clinical trials and to accelerate the development of new cancer therapies will be summarized
Identification and expression analysis of the ZF-HD gene family in castor
No full text[Objective] The ZF-HD family genes of castor were identified and analyzed by sequence analysis,
phylogenetic analysis, promoter and expression pattern analysis, to provide a reference for further
study of the function of ZF-HD genes in castor. [Methods] Based on the whole genome data of castor,
ZF-HD genes were identified, and bioinformatics and expression analysis under abiotic stress were carried
out. [Results] A total of 13 ZF-HD genes were identified from castor, which were unevenly distributed on
Chr1-Chr10. All 13 RcZF-HD proteins were hydrophilic unstable proteins, and were localized in the nucleus.
The RcZF-HD genes were clustered into three subgroups, and the number of genes in each group was
different. The gene structure was conserved within the group but diversified between groups. RcZF-HD
genes had a large number of tissue-specific elements, stress response elements, and hormone response elements.
The ZF-HD genes were widely responsive to abiotic stresses and their expression was tissue-specific.
[Conclusion] A total of 13 ZF-HD genes were identified in castor. It is known that members of the
castor ZF-HD gene family play an important role in responding to abiotic stress, this research provides
theoretical basis for the study of castors stress resistance
Neutralizing antibodies against the Omicron variant in CV/ZF/ZF (D0-D56-M6) regimen.
No full text(A) GMTs of neutralizing antibodies against the Omicron variant 14 days post first and second boost. (B) Geometric mean ratios of neutralizing antibodies against wild-type relative to Omicron 14 days post first and second boost. The numbers indicate the geometric mean ratios. Cutoff (1:4) refers to the detection limit. Horizontal bars show geometric mean titer and error bars show 95% CI. CV/ZF/ZF (D0-D56-M6) = receiving Convidecia/ZF2001/ZF2001 at day 0, day 56, and month 6. ****P (TIF)</p
One-hybrid system for detection of ZF–DNA interactions () Schematic of genetic selection system for interrogating ZF–DNA interactions
No full text<p><b>Copyright information:</b></p><p>Taken from "Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells"</p><p>Nucleic Acids Research 2005;33(18):5978-5990.</p><p>Published online 26 Oct 2005</p><p>PMCID:PMC1270952.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () Plasmids for one-hybrid genetic selection system. The reporter gene, either chloramphenicol acetyltransferase (CAT), or GFP is located downstream from a weak derivative promoter (P) on pDB series plasmids. A 9 bp target site for binding by the ZF is located at a specific distance from the start of transcription. On the pA series of plasmids, the gene for the ZF is fused to a fragment of the α-subunit of RNA polymerase ([]) via a sequence coding for an amino acid linker. Binding of the RpoA[1–248]–ZF fusion to the 9 bp site in the reporter plasmid recruits the other RNA polymerase subunits to stimulate transcription of the reporter gene
Differential expression of <i>Populus</i> C2HC2-ZF genes under different stress conditions.
No full text<p>The heatmap was visualized using the exHeatmap tool in the PopGenIE v2 database. Blue and red indicate low and high levels of transcript abundances, respectively. <b>A.</b> Heatmap showing 92 <i>Populus</i> C2HC2-ZF genes in leaves and roots under drought stress. <b>B.</b> Heatmap showing 92 <i>Populus</i> C2HC2-ZF genes under beetle and mechanical stress.</p
Phylogenetic tree of C2H2-ZF proteins from <i>Populus</i> and rice.
No full text<p>One hundred and nine C2H2-ZF proteins in <i>Populus</i> and 189 in rice were aligned using Clustal X 1.83 and the phylogenetic tree was constructed using MEGA 5.0 by the neighbor-joining method. The Bootstrap value was 1,000 replicates. The rice proteins start with Z and are marked with a solid dot to distinguish them from the <i>Populus</i> C2HC2-ZF proteins. The three plant-specific clusters were designated as a, b and c, and indicated in a specific color.</p
Chromosomal locations of <i>Populus</i> C2HC2-ZF genes.
No full text<p>One hundred and nine C2HC2-ZF genes were mapped to 19 linkage groups (LG). A schematic view of chromosome reorganization caused by recent whole-genome duplication in <i>Populus</i> is shown (adapted from [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134753#pone.0134753.ref032" target="_blank">32</a>]). Segmental duplicated homologous blocks are indicated by the same color. The scale represents mega bases (Mb). The LG numbers are indicated above each bar.</p
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