15,429 research outputs found
Hair analysis for drugs of abuse
No full textThis thesis covers a range of important issues in hair analysis and includes 27 scientific works in which the name of the candidate was either listed as the first author or as the major contributor. The work presented in this thesis involved the development of a series of analytical methods to detect trace amounts of drugs in hair and also investigated the mechanisms by which drugs may be incorporated into hair. The major areas covered in this study can be summarized as follows:
1. The methods for the identification and quantification of opiates, amphetamines, ketamine, cannabis, cocaine, benzodiazepines, antidepressants, antipsychotics, and anabolic steroids in hair were developed using gas chromatography–mass spectrometry (GC-MS), liquid chromatography–tandem mass spectrometry (LC-MS/MS) and gas chromatography–tandem mass spectrometry (GC-MS/MS). With GC-MS methods, the limits of detection were 0.1-0.5 ng mg-1 of hair for antidepressants and antipsychotics. For illegal drugs, hair specimens were analyzed by GC-MS with limits of detection of 0.02-2ng mg-1. GC/MS/MS is more sensitive than GC-MS to detect these drugs in hair. The lower limits of detection ranged from 0.001 to 0.020 ng mg-1 for 21 anabolic androgenic steroids and their esters in hair using liquid chromatographic-tandem mass spectrometric method. And the limits of detection ranged from 0.2 to 5 pg mg-1 for benzodiazepines in hair. Tandem mass spectrometry is characterized by its sensitivity, selectivity and specificity, which makes it particularly suitable for the analysis of trace amount of target analytes in hair.
2. Usually, screening for drugs of abuse is the first step in clinical and forensic toxicology. There are a large number of controlled substances and doping agents and novel compounds, which have yet to be characterised. A series of screening methods for drugs of abuse in hair were developed using LC-MS/MS and GC-MS/MS. Using our own library of MRM transitions, the optimum collision energies selected for each transition and retention times were set up. These methods have been applied successfully in forensic casework.
3. Of growing importance to the field of hair analysis is the detection of metabolites related to the parent drugs. Demonstrating the presence of a metabolite of a drug (such as, heroin, amphetamines, cocaine, meperidine, ketamine, triazolam or psychotropic drugs) provides compelling evidence for exposure to the parent drug, and permits distinction between external contamination from ingestion and facilitation of the interpretation of results. The presence of antidepressant and antipsychotic drugs and their metabolites in the hair of psychiatric patients was investigated using GC-MS-EI and GC-MS-PCI. The parent drug and its major metabolite, such as opiates (morphine, 6-acetylmorphine), methamphetamine (methamphetamine, amphetamine), ketamine (ketamine, norketamine), cocaine (cocaine, benzoecognine), meperidine (meperidine, normeperidine), triazolam (triazolam, α-hydroxytriazolam), and clonazepam (clonazepam, 7-aminoclonazepam) were quantified in authentic hair samples simultaneously. The differences were finding in the ratio of parent drug to metabolite. For illegal drugs, the concentrations of parent drugs were higher than that of their metabolites. The results of triazolam and clonazepam were contrary. These data are suitable reference values and are the basis for the interpretation of results.
4. The mechanisms by which drugs are incorporated into hair are not fully understood. Based on experiments with guinea pigs with black, white, or brown hair, the mechanisms of incorporation of cocaine, methamphetamine, ketamine, triazolam and anabolic steroids into hair were investigated. The concentrations of drugs in hair were found to be related to physicochemical properties of drugs. The parent drugs were the predominant analytes in hair. There was an obvious relationship between the concentration of drugs in hair and hair pigmentation. The concentrations of drugs deposited in black hair was found to be higher than that in brown and white hair samples, even when comparing results using hairs on the same multicoloured animal body. This work confirmed that melanin affinity is a governing factor in drug incorporation into hair shafts. These studies on the distribution of drugs in the hair shaft and how their concentration changes along the shaft provide information relevant to the time of ingestion and substance use/abuse.
5. In recent years an increase in drug-facilitated sexual assault (DFSA) has been reported. Segmental hair analysis has proved useful in widening the window of detection, as blood and urine analyses are of limited use, due to the long delays between the actual assaults and obtaining samples from suspects that are frequently encountered in investigations of such crimes. In China, benzodiazepines are the most frequently observed compounds in cases of drug-facilitated crime. In a paper reported here, 14 volunteers ingested a single 1-6 mg estazolam tablet to permit the evaluation of segmental hair analysis after a single drug dosage. Hair was collected one month after administration of the drug. All the proximal segments tested positive for estazolam. With increased dosage, estazolam could be detected in the 2-4 cm segments nearest the hair root in some subject’s hair shafts. In some cases, the 4-6 cm segments also tested positive. Hair analysis was applied to samples from two authentic criminal cases. A significant variation was observed between those obtained from previous studies and the results presented here. The intersubject variability in segmental analysis can be explained mainly due to melanin content and diffusion from sweat or other secretions during formation of the hair shaft. However, more substantial procedural and interpretation guidelines are required to use segmental hair analysis in drug-facilitated crimes. On the other hand, the minimal dosage for detection, which is a critical but previously unknown threshold value of fundamental importance in hair analysis, was determined for triazolam and ketamine in guinea pig hair.
6. Doping with endogenous anabolic steroids is one of the most serious drug issues in sports today. The measurement of anabolic steroid levels in human hair permits the distinction between pharmaceutically produced steroids and naturally occurring steroids. Full-length hair samples were taken at the skin surface from the vertex of 39 males, 30 females and 11 children from China. None of the subjects were professional athletes. Testosterone and dehydroepiandrosterone were detected in all the hair segments. The physiological concentrations of testosterone were in the range 0.8-24.2 pg mg-1, 0.1-16.8 pg mg-1 and 0.2-11.5 pg mg-1 in males, females and children, respectively. However, the mean values of dehydroepiandrosterone were much higher than those for testosterone. This is the first investigation into the physiological concentrations of anabolic steroids in human hair in Chinese subjects. These data provide suitable reference values and form the basis for the interpretation of results from investigations into the abuse of endogenous anabolic steroids.
In conclusion, the work presented in this study demonstrates that there was a good correlation between the concentration of drugs in hair and drug dosage. There was an obvious relationship between hair drug concentration and hair colour. Melanin affinity is shown to be a governing factor in determining drug incorporation into hair, and the concentration of drugs deposited in black hair was found to be higher than that in brown and white hair samples. This thesis provides data that will be useful in the application of hair analysis regarding drugs of abuse and in the interpretation of toxicological results
L'ordre secret d'exiler les dissidents
No full textDa Xiang, Ping An, Zhengming. L'ordre secret d'exiler les dissidents. In: Perspectives chinoises, n°11-12, 1993. pp. 6-9
Lun Jin Ping Mei
No full textBen shu ping lun le shi dai zuo zhe, si xiang yi shu, ban ben te dian he yu ci shi liao deng nei ron
The politics of fashion: perceptions of power in female clothing and ornamentation as reflected in the sixteenth-century Chinese novel Jin Ping Mei
No full textThis thesis examines issues of female power and influence in sixteenth-century China focusing on how women and their roles were perceived in the changing social environment of the mid-late Ming dynasty. Using aspects of a New Historicist approach, information from contemporary literary and historical sources are analysed alongside each other. With its emphasis on the lives of women and preoccupation with the description of material objects, the late Ming novel Jin Ping Mei forms an important element in the thesis. China in the sixteenth century saw expanding urbanisation, the emergence of a new wealthy merchant class, increasing visibility of women and a questioning of traditional morality. Fashion consciousness, as one of the most conspicuous aspects of the new material culture, is a possible indicator of these trends. Traditional Western theories contend that fashion began in the particular context of Renaissance Europe. However, this study argues that a similar fashion awareness existed in China too, and was manifested in a competitive striving for social status, in this case specifically among women. In contrast to previous studies which downplayed the impact women had on defining traditional Chinese culture, this thesis demonstrates how women and their sartorial choices began to redefine the boundaries of material culture, influencing literati discourse which, in turn, re- influenced female behaviour
Mao yi yu he ping = Trade and peace
No full textBen shu you mao yi pian, Zhan zheng pian, He ping pian zu cheng. Bao kuo mao yi yu he ping xiang rong gong sheng, Di li da fa xian zhi qian de mao yi yu fen zheng, Xi fang zi you mao yi ban sui mao yi zheng duan er jue qi, Jin dai zhan zheng he mao yi shi zhong guo rong ru shi jie, Feng yun bian huan de ban ge shi ji, Mao yi zao jiu zhan hou de xiang dui he ping, Mao yi yu he ping de wei lai fa zhan gong qi zhang nei ron
Two new records of the Family Celaenopsidae (Acari: Mesostigmata) from China, with description of a new species
No full textXu, Yun, Li, Xiang, Zhang, Fei-Ping (2019): Two new records of the Family Celaenopsidae (Acari: Mesostigmata) from China, with description of a new species. Zootaxa 4604 (2): 326-334, DOI: 10.11646/zootaxa.4604.2.
A new species and a key to known species of the genus Atractomorpha (Orthoptera, Acridoidea, Pyrgomorphidae) from China
No full textYin, Xiang-Chu, Shi, Jian-Ping (2007): A new species and a key to known species of the genus Atractomorpha (Orthoptera, Acridoidea, Pyrgomorphidae) from China. Zootaxa 1453 (1): 63-68, DOI: 10.11646/zootaxa.1453.1.
Radial Density and Temperature Profiles of the Intracluster Gas Constructed Jointly from the X-Ray Surface Brightness Measurement and the Universal Density Profile
No full textIsodon delavayi (Ocimeae, Nepetoideae, Lamiaceae): a new species from Yunnan Province, Southwest China
No full textChen, Ya-Ping, Hu, Guo-Xiong, Xiang, Chun-Lei (2014): Isodon delavayi (Ocimeae, Nepetoideae, Lamiaceae): a new species from Yunnan Province, Southwest China. Phytotaxa 156 (5): 291-297, DOI: 10.11646/phytotaxa.156.5.5, URL: http://dx.doi.org/10.11646/phytotaxa.156.5.
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