111 research outputs found
Ji yu jin chang sheng xue xuan fu de zi yun xing he zi fu dong er wei zhi dong qi
No full textM.Phil.Acoustic radiation force in the near-field of a vibrating source can be utilized to lift and transport objects, which provides a non-contact driving technology in addition to maglev. This research presents a novel self-running planar motion stage utilizing near-field acoustic transportation phenomenon and coupled resonant vibration of the device.Finite element analysis models have been established to find the needed resonant modes and optimize the design of the structure. The first longitudinal and the second bending modes are designed to have an identical frequency to create elliptical vibration of the structure, which can provide vertical levitation force as well as the propulsion force. A dynamic model based on the Reynolds Equation has been established to study the levitation and driving mechanism of the proposed device. The levitation and driving forces are analyzed; and the relationship between the driving force and the input phase difference is investigated to optimize the following position control. A capacitance surface encoder is proposed to provide the levitation height and two dimensional position feedback for the stage. The encoder design is based on the measurement of capacitance change between the driving stage and the metal sensing plates embedded in the ground. The lateral position (in the XY plane) of the circular stage can be uniquely determined based on the capacitance values.A prototype including the stage, encoder, and controller is implemented and characterized to demonstrate the performance of the proposed design. Two motion characteristics of the stage are first tested: one is the influence of the excitation voltage amplitude on the levitation height, and the other is the influence of the amplitude and phase angles of the excitation signals on the velocity and thrust force. In the calibration of the encoder, the calculated coordinates are firstly compensated for the scaling and the misalignment of the encoder axis and the precision stage. And the systematic errors from the nonlinear features are identified and fitted to a third-order polynomial equation in each axis. In the control scheme, the capacitance encoder works as the feedback module, and a LabVIEW program is used for date acquisition and processing. The stage is tested and demonstrated to follow a circular trajectory with a radius of 3.5mm.This work investigates a novel self-floating and self-running two-dimensional actuator using near-field acoustic levitation and coupled resonant vibration of the device. Compared with the conventional designs based on near-field acoustic levitation, the proposed design eliminates any external component such as guide rail, which contributes to bigger working space. This design also has two-dimensional motion capability with higher load capacity and energy efficiency. A dynamic model based on the Reynolds Equation is established to study the driving mechanism. A capacitance surface encoder is proposed to provide the two dimensional position feedback for the stage. A prototype including the stage, encoder, and controller is implemented and characterized to demonstrate the capability of the proposed design.振動源在近場中產生的輻射力可以用於提升和運輸物體，這現象可用於非接觸驅動技術，並有別於磁懸浮技術。本研究提出了一種新型的自運行平面運動機構，該機構利用了近場聲學傳播現象和裝置的耦合諧振。通過有限元分析可以找到機構的共振模態，並且用於指導結構的優化設計。通過結構設計，使得一階縱向模態和二階橫向模態具有相同的振動頻率，以產生橢圓振動，橢圓振動同時提供懸浮立和前進驅動力。建立了基於雷諾方程的運動模型，來分析機構的懸浮和運動機制。通過分析懸浮力和驅動力，獲得驅動力與輸入信號相位差之間的關係，用於之後定位平臺的位置控制。基於表面電容的編碼器被用於定位平臺的位置反饋，這個反饋包括了平臺的懸浮高度以及它的二維平面位置。編碼器可以測量懸浮平臺和固定在地上的金屬感應板間的電容。基於測得的電容值，通過相應的計算，可以得出平臺在XY平面內的確定位置。建立了包括運動器，編碼器和控制器在內的實驗模型，用於驗證設計的性能。首先是關於驅動器運動性能的測定，第一個是電壓大小對懸浮高度的影響，第二個是電壓大小以及電壓相位角對於運動速度和驅動力的影響。在編碼器的校準過程中，首先補償了由於編碼器和精密平臺錯位以及縮放而引起的誤差， 然後運用三階多項式來擬合每個軸的非線性系統誤差。在控制方案中，電容式編碼器起反饋作用，一個LabVIEW程序用於對電信號的處理。在運動測試中，平臺行走了一個半徑為3.5mm的圓形軌跡。這項研究成功研發了一種新型的自懸浮和自運動的二維運動機構，這個機構運用了近場超聲懸浮和耦合共振效應。與傳統的近場超聲懸浮裝置相比，新的設計不需要導軌之類的任何外部部件，這有助於提供更大的工作空間。該設計具有二維運動能力，並有更高的負載能力和能量效率。建立了基於雷諾方程的運動模型來研究其運動機制。用電容編碼器來當作二維定位平臺的位置反饋。包括運動機構，編碼器和控制器在內的實驗模型被建立，用於驗證設計的性能。Chen, Keyu.Thesis M.Phil. Chinese University of Hong Kong 2017.Includes bibliographical references (leaves 56-60).Abstracts also in Chinese.Title from PDF title page (viewed on 06, February, 2020).Chen, Keyu
Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk
No full textLateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA. was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 x 10(2) cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples
Nanozymatic activity of Zr-based MOFs: Tuning the coordinatively unsaturated metal sites for enhancing hydrolytic activity toward peptide bonds
No full textObjective: To develop efficient and stable artificial proteases and investigate the relationship between coordinatively unsaturated metal sites and hydrolysis activity of peptide bond. Methods: Three zirconium-based metal-organic frameworks （Zr-MOFs） with different coordinatively unsaturated sites （12-connected Zr-MOF, 6-connected Zr-MOF, and 4-connected Zr-MOF） were prepared to modulate protein hydrolase activity. The protein hydrolase activity of three Zr-MOFs was evaluated by the hydrolysis rate of bis-glycopeptide （Gly-Gly） as an indicator. Subsequently, soy protein, surimi protein and casein were hydrolyzed using the best performing protein hydrolase nanoenzymes, and the hydrolysis products were separated using SDS-polyacrylamide gel electrophoresis, and the hydrolysis efficiency of the three proteins by artificial proteases was investigated by staining the separated bands with Komas Brilliant Blue. Results: Among the three Zr-MOFs proteases, the 6-connected Zr-MOF showed the highest hydrolysis （52%） efficiency toward the Gly-Gly. The hydrolysis reaction rate was increased 2.63×103 times compared the uncatalyzed hydrolysis. In addition, the 6-connected Zr-MOF could respectively hydrolyze three commonly proteins in the food industry and had the highest catalytic effect on surimi protein. Conclusion: These results revealed that the ability of Zr-MOF to hydrolyze peptide bonds could be enhanced by tuning the coordinatively unsaturated sites. The study provided a new idea for the application of artificial proteases in food industry
Author response image 1. Author response
No full textAnkyrin adaptors together with their spectrin partners coordinate diverse ion channels and cell adhesion molecules within plasma membrane domains and thereby promote physiological activities including fast signaling in the heart and nervous system. Ankyrins specifically bind to numerous membrane targets through their 24 ankyrin repeats (ANK repeats), although the mechanism for the facile and independent evolution of these interactions has not been resolved. Here we report the structures of ANK repeats in complex with an inhibitory segment from the C-terminal regulatory domain and with a sodium channel Nav1.2 peptide, respectively, showing that the extended, extremely conserved inner groove spanning the entire ANK repeat solenoid contains multiple target binding sites capable of accommodating target proteins with very diverse sequences via combinatorial usage of these sites. These structures establish a framework for understanding the evolution of ankyrins' membrane targets, with implications for other proteins containing extended ANK repeat domains.</p
Effects of corrective feedback on EFL speaking task complexity in China’s university classroom
No full textCorrective feedback (CF) and task complexity are two important pedagogical topics in second language acquisition research in recent years, but there is few research investigating effects of CF on speaking task complexity in China’s university classroom settings. This research, through conducting different versions of speaking task experiments among 24 university students in China, explores the effect of teachers’ CF on English as a Foreign Language (EFL) speaking task complexity. According to the analysis of first-hand data, this research finds CF has different effects on EFL oral production with different task complexity. In simple speaking task, the effects of five kinds of CF (from largest to smallest) are listed as follows: clarification quest, metalinguistic feedback, recast, repetition and confirmation check. Regarding complex speaking task, the effects of five categorized CF are ranked from largest to smallest as follows: metalinguistic feedback, confirmation check, recast, clarification request and repetition. Improving to provide CF in pedagogical practice is an important contribution to promote EFL speaking task, so, on the basis of above research results, appropriate ways and forms of providing CF are expected to promote efficiency of CF in EFL classroom under the context of Chinese university classroom
A Mean Field Game Approach to Relative Investment-Consumption Games with Habit Formation
No full textThis paper studies an optimal investment-consumption problem for competitive
agents with exponential or power utilities and a common finite time horizon.
Each agent regards the average of habit formation and wealth from all peers as
benchmarks to evaluate the performance of her decision. We formulate the
n-agent game problems and the corresponding mean field game problems under the
two utilities. One mean field equilibrium is derived in a closed form in each
problem. In each problem with n agents, an approximate Nash equilibrium is then
constructed using the obtained mean field equilibrium when n is sufficiently
large. The explicit convergence order in each problem can also be obtained. In
addition, we provide some numerical illustrations of our results.Comment: arXiv admin note: substantial text overlap with arXiv:2206.13341 by
other author
An Evaluation of Environmental Governance in Urban China Based on a Hesitant Fuzzy Linguistic Analytic Network Process
No full textThe aim of this study is to evaluate the performance of urban environmental governance by developing hesitant fuzzy linguistic analytic network process (HFL-ANP). The study bridges the gaps in current knowledge in the following ways: the study methodically develops the HFL-ANP method to evaluate and pick the optimal environmental governance strategy from alternatives; theoretically, network structure of evaluation indicators system on environmental governance is constructed, and the objective and subjective information in the evaluation process of environmental governance is combined. In detail, based on the environmental Kuznets curve (EKC) and the pollution haven hypothesis, the paper constructs the evaluation indexes system of environmental governance and takes observation time length into consideration. Then, we choose three urban cases of environmental governance by exploring the existing literature. Furthermore, we develop the HFL-ANP method and apply it to the cases. The study calculates the initial weights of all indexes by using multiplicative consistency of the HFL preference relation, and derives the decision matrix through combining objective information with subjective information of environmental governance. Finally, we come to the following conclusions: ANP network stricture is close to real-world practical problems and provides the basis for HFL-ANP method; HFL-ANP is a very suitable method of assessing environmental governance; and based on the urban cases of environmental governance, Shanghai is the optimal alternative. In addition, this indicator system can only be applied to cities in China, and the administrative hierarchy of policies has not been considered by this method. Thus, future studies should expand this method and indicator network to contain different countries and different administrative hierarchy
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