127,074 research outputs found
SPECIFIC RECOGNITION OF N-ACETYLNEURAMINIC ACID IN THE G(M2) EPITOPE BY HUMAN G(M2) ACTIVATOR PROTEIN
No full textG(M2) Activator is a low molecular weight protein cofactor that stimulates the enzymatic conversion of G(M2) into G(M3) by human beta-hexosaminidase A and also the conversion of G(M2) into G(A2) by clostridial sialidase (Wu, Y.-Y., Lockyer, J. M., Sugiyama, E., Pavlova, N. V., Li, Y.-T., and Li, S.- C. (1994) J. Biol. Chem. 269, 16276-16283). Among the five known activator proteins for the enzymatic hydrolysis of glycosphingolipids, only G(M2) activator is effective in stimulating the hydrolysis of G(M2). However, the mechanism of action of G(M2) activator is still not well understood, Using a unique disialosylganglioside, GalNAc-G(D1a), as the substrate, we were able to show that in the presence of G(M2) activator, GalNAc-G(D1a) was specifically converted into GalNAc-G(M1a) by clostridial sialidase, while in the presence of saposin B, a nonspecific activator protein, GalNAc-G(D1a) was converted into both GalNAc-G(M1a) and GalNAc-G(M1b). individual products generated from GalNAc-G(D1a) by clostridial sialidase were identified by thin layer chromatography, negative secondary ion mass spectrometry, and immunostaining with a monoclonal IgM that recognizes the G(M2) epitope. Our results clearly show that G(M2) activator recognizes the G(M2) epitope in GalNAc-G(D1a). Thus, G(M2) activator may interact with the trisaccharide structure of the G(M2) epitope and render the GalNAc and NeuAc residues accessible to beta-hexosaminidase A and sialidase, respectively
Rude C. G: Rude, A/S; Maskinforretnig Christiana
No full textRUDE C. G: RUDE, A/S; MASKINFORRETNIG CHRISTIANA
Rude C. G: Rude, A/S; Maskinforretnig Christiana ( -
Characterization of an alternatively spliced G(M2) activator protein, G(M2A) protein - An activator protein which stimulates the enzymatic hydrolysis of N-acetylneuraminic acid, but not N-acetylgalactosamine, from G(M2)
No full textG(M2) activator protein is a protein cofactor which stimulates the enzymatic hydrolysis of both GalNAc and NeuAc from G(M2). We have previously isolated two cDNA clones, G(M2) activator cDNA and G(M2A) cDNA, for human G(M2) activator protein (Nagarajan, S., Chen, H.-C., Li, S.-C., Li, Y.-T., and Lockyer, J. M. (1992) Biochem. J. 282, 807-813). G(M2A) mRNA is an RNA alternative splicing product that contains exons 1, 2, 3, and intron 3 of the genomic DNA sequence of G(M2) activator protein (Klima, H., Tanaka, A., Schnabel, D., Nakano, T., Schroder, M., Suzuki, K., and Sandhoff, K. (1991) FEES Left. 289, 260-264). G(M2A) cDNA encodes a protein (G(M2A) protein) containing 1-109 of the 160 amino acids of human G(M2) activator protein, plus a tripeptide (VST) encoded by intron 3 at the COOH terminus. Thus, G(M2A) protein can be regarded as a form (truncated version) of G(M2) activator protein. We have expressed G(M2A) cDNA in Escherichia coli using pT7-7 as the vector. The recombinant G(M2A) protein was purified to an electrophoretically homogeneous form and was found to stimulate the hydrolysis of NeuAc from G(M2) by clostridial sialidase, but not the hydrolysis of GalNAc from G(M2) by beta-hexosaminidase A. Like G(M2) activator protein, G(M2A) protein also specifically recognized the terminal G(M2) epitope in GalNAc-GD1a and stimulated the hydrolysis of only the external NeuAc from this ganglioside by clostridial sialidase. These results enabled us to discern the enzymatic hydrolyses of GalNAc and NeuAc from the G(M2) epitope and established that the NeuAc recognition domain of G(M2) activator protein is located within amino acids 1-109. The presence of G(M2A) mRNA in human tissues and the selective stimulation of NeuAc hydrolysis by G(M2A) protein indicate that this activator protein may be involved in the catabolism of G(M2) through the asialo-G(M2) pathway
Optimum structures of digital controllers in sampled-data systems: a roundoff noise analysis
Get PDFIn this paper, the effect of roundoff noise in a digital controller is analyzed for a sampled-data system in which the digital controller is implemented in a state-space realization. A new measure, called averaged roundoff noise gain, is derived. Unlike the traditionally used measure, where the analysis is done based on an equivalent digital control system, this newly defined averaged roundoff noise gain allows us to take consideration of the inter-sample behavior. It is shown that this measure is a function of the state-space realization. Noting the fact that the state-space realizations of a digital controller are not unique, the problem of optimum controller structure is to identify those realizations that minimize the averaged roundoff noise gain subject to the -scaling constraint which is for preventing the signals in the controller from overflow. An analytical solution to the problem is presented and a design example is given. Both theoretical analysis and simulation results show that the optimum controller realizations obtained with the proposed approach are superior to those obtained with the traditional analysis based on a digital control system
Riebeck-Kerzen Altbewährtes Erzeugnis Grösster Jahresumsatz A.Riebecksche Montanwerke A.-G. Halle A/S
No full textRIEBECK-KERZEN ALTBEWÄHRTES ERZEUGNIS GRÖSSTER JAHRESUMSATZ A.RIEBECKSCHE MONTANWERKE A.-G. HALLE A/S
Riebeck-Kerzen Altbewährtes Erzeugnis Grösster Jahresumsatz A.Riebecksche Montanwerke A.-G. Halle A/S ( -
Rome. The Centre(s) Elsewhere
No full textRome: The Centre(s) Elsewhere is the result of a Berlage Institute postgraduate studio led by Pier Vittorio Aureli and Martino Tattara, in collaboration with Gabriele Mastrigli. The book proposes both the definition of a new urban strategy for the city of Rome and a critical framework through which to understand the city’s history. Going beyond the grandeur of the past and the uncertainty of the present, Rome: The Centre(s) Elsewhere offers a project for the city’s possible future by reclaiming the greatest and yet currently neglected asset of its urban structure: the consular roads system. The book is a critical and concise pamphlet about urban design and the project of the city. It represents an attempt to gather into one approach the issues of large-scale design, political thinking, and urban history. The publication is based on a research at the Berlage Institute Rotterdam entitled Rome: The Centre(s) Elsewhere, which took place during the 2008–2009 academic year. The research was presented first at The Berlage Institute in Rotterdam then in an international conference and an exhibition entitled Rome: The Centre(s) Elsewhere, held at the Casa dell’Architettura from 9 June to 7 July 2010 and organized as part of the Festa dell’Architettura of Rome 2010. Together with being co-curator of the research exhibition, Gabriele Mastrigli is author of the exhibition design
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Time-dependent stochastic shortest path(s) algorithms for a scheduled transportation network
No full textFollowing on from our work concerning travellers’ preferences in public transportation networks (Wu and Hartley, 2004), we introduce the concept of stochasticity to our algorithms. Stochasticity greatly increases the complexity of the route finding problem, so greater algorithmic efficiency becomes imperative. Public transportation networks (buses, trains) have two important features: edges can only be traversed at certain points in time and the weights of these edges change in a day and have an uncertainty associated with them. These features determine that a public transportation network is a stochastic and time-dependent network. Finding multiple shortest paths in a both stochastic and time-dependent network is currently regarded as the most difficult task in the route finding problems (Loui, 1983). This paper discusses the use of k-shortest-paths (KSP) algorithms to find optimal route(s) through a network in which the edge weights are defined by probability distributions. A comprehensive review of shortest path(s) algorithms with probabilistic graphs was conducted
Optimal realisations of fixed-point implemented digital controller with the smallest dynamic range
No full textJ Wu
No full textResearch School of Social Sciences - Research Scholars - S. Matsuura, D. Hindley, J. D. Playford, K. G. Jones, G. A. Waterson, Dr. R. R. Brown, Dr. Prgybylski, Dr. D. A. Low, Dr. W. E. Salter, S. R. Adke, Mr. G. Docker, A. R. Barcan, Dr. J. C. Harsanyi, Dr. K. H. Burley, Miss Ann Myers, G. P. King, J. Stockwin, Mr. R. G. Boyd, Dr. H. O. Pappe, Dr. J. A. Barnard, R. J. Lawrence, K. G. Pont, J. Caldwell, N. G. Cain, D. W. Dockrill, C. S. Ross, Abraham Harari, I. Fairbanks, Mr. G. Pursell, F. L. Jones, M. Wu, David B. Heron, D. K. Singh, H. Yuan T'ien, Owen Michael Roe, F. S. Henr
Development of composite calibration standard for quantitative NDE by ultrasound and thermography
Get PDFInspection of aircraft components for damage utilizing ultrasonic Non-Destructive Evaluation (NDE) is a time intensive endeavor. Additional time spent during aircraft inspections translates to added cost to the company performing them, and as such, reducing this expenditure is of great importance. There is also great variance in the calibration samples from one entity to another due to a lack of a common calibration set. By characterizing damage types, we can condense the required calibration sets and reduce the time required to perform calibration while also providing procedures for the fabrication of these standard sets. We present here our effort to fabricate composite samples with known defects and quantify the size and location of defects, such as delaminations, and impact damage. Ultrasonic and Thermographic images are digitally enhanced to accurately measure the damage size. Ultrasonic NDE is compared with thermography.This proceeding may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing.
This proceeding appeared in Dayal, Vinay, Zach G. Benedict, Nishtha Bhatnagar, and Adam G. Harper. "Development of composite calibration standard for quantitative NDE by ultrasound and thermography." In AIP Conference Proceedings, vol. 1949, no. 1, p. 060006. AIP Publishing LLC, 2018, and may be found at
DOI: 10.1063/1.5031552.
Copyright 2018 The Author(s).
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