103 research outputs found
Clinical progress on restoration by chairside CAD/CAM inlay
No full textTooth defects due to dental caries, trauma, abrasion, etc., are extremely common and can be treated by di⁃
rect or indirect restoration. Compared with resin directly filling the body, an inlay can better restore the occlusal contact
relationship and the adjacent surface contact relationship and has good mechanical properties. In recent years, with the
development of ceramic materials and bonding systems and the popularity of chairside CAD/CAM technology, the chair⁃
side CAD/CAM porcelain inlay restoration program has been well received by doctors and patients because of its accura⁃
cy, convenience, aesthetics, hardness and stability, and this program is widely used clinically. This review covers the re⁃
search status of various aspects such as indications and contraindications for chairside CAD/CAM inlay restoration, pre⁃
restoration preparation, tooth preparation, hole type, impression taking and design, porcelain block selection, bonding,
polishing, postoperative doctors instructions, and common postoperative complications. It is expected to provide a refer⁃
ence for the clinical application of and research on chairside CAD/CAM inlay restoration technology
Phage therapy as an alternative strategy for oral bacterial infections: a systematic review
No full textAbstract Background Oral infectious diseases, such as dental caries, periodontitis and periapical periodontitis, are often complicated by causative bacterial biofilm formation and significantly impact human oral health and quality of life. Bacteriophage (phage) therapy has emerged as a potential alternative with successful applications in antimicrobial trials. While therapeutic use of phages has been considered as effective treatment of some infectious diseases, related research focusing on oral infectious diseases is few and lacks attention. Therefore, a systematic review was conducted to comprehensively evaluate the overall efficacy of phages in reducing bacterial infections associated with various oral diseases. Methods A systematic search of PubMed, MEDLINE and Web of Science for literature published up to May 2024 was conducted according to inclusion criteria to identify studies assessing bacteriophages as potential therapy for oral infectious diseases. A total of four authors assessed study eligibility and performed data extraction. Results A total of 487 articles published between 1975 and 2024 were retrieved. Among the 10 eligible reports, preliminary studies have been conducted on seven types of phages and reported their antibacterial effect. To be more specific, 3 contained data on dental caries (n = 32), 5 focused on periodontitis (n = 105) and 2 examined periapical diseases (n = 7). The majority of publications (9 out of 10) discussed the impact of phages on biofilm formation. Only one report (1 out of 10) mentioned the safety concern in phage application. Conclusions This review strongly suggests that phages isolated from oral cavity with certain characteristics can be highly effective and are considered suitable candidates for phage therapy in treating oral/odontogenic infections caused by bacteria
Clinical effect of semiconductor laser combined with total glucosides of paeony capsules for the treatment of erosive oral lichen planus
No full textObjective To investigate the short⁃term clinical effect of semiconductor laser exposure combined with
total glucosides of paeony (TGP) capsules for the treatment of erosive oral lichen planus (OLP). Methods Sixty⁃four
patients with erosive oral lichen planus were randomly divided into two groups: the experimental group and the control
group. Patients in the control group were treated with TGP capsules, while patients in the experimental group were treat⁃
ed with TGP capsules and semiconductor laser irradiation. The clinical effects were evaluated 3 months after treatment.
The data were analyzed using the SPSS 17.0 software package. Results Three months after treatment, the effective
rate in the experimental group was 90.6%, which was significantly higher than that in the control group (59.4%, χ2＝
5.62, P < 0.05). The physical condition and visual analogue scale (VAS) scores in the experimental group were 2.17 ±
1.49 and 1.25 ± 1.29, respectively. The physical condition and VAS scores in the control group were 3.55 ± 1.41 and
2.09 ± 1.24, respectively. The physical condition and VAS scores in both groups were significantly higher after treat⁃
ment than before (P < 0.05). Three months after treatment, the physical condition score (t＝3.805) and VAS score (t＝
2.655) in the experimental group were significant higher than those in the control group (P < 0.05). Conclusion Semi⁃
conductor laser irradiation combined with TGP capsules can improve the short⁃term clinical efficacy in the treatment of
erosive OLP
Study on the structure and hydraulic performance of the hydraulic pressure emitter of reverse gear
No full textcircRNA Expression Profile in Dental Pulp Stem Cells during Odontogenic Differentiation
No full textIntroduction. Odontogenic differentiation of human dental pulp stem cells (hDPSCs) is a key step of pulp regeneration. Recent studies showed that circular RNAs (circRNAs) have many biological functions and that competing endogenous RNA (ceRNA) is their most common mechanism of action. However, the role of circRNAs in hDPSCs during odontogenesis is still unclear. Methods. Isolated hDPSCs were cultured in essential and odontogenic medium. Total RNA was extracted after 14 days of culture, and then, microarray analysis was performed to measure the differential expressions of circRNAs. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was then performed to validate the microarray results. Based on microarray data from this study and available in the database, a ceRNA network was constructed to investigate the potential function of circRNAs during odontogenesis. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to investigate the potential correlation between signaling pathways and circRNAs. In addition, qRT-PCR and Western blot analysis were used to explore the function of hsa_circRNA_104101. Results. We found 43 upregulated circRNAs and 144 downregulated circRNAs during the odontogenic differentiation process (fold change>1.5 and <-1.5, respectively; P<0.05). qRT-PCR results were in agreement with the microarray results. Bioinformatic analysis revealed that the Wnt signaling pathway and the TGF-β signaling pathway, as well as the other pathways associated with odontogenic differentiation, were correlated to the differentially expressed circRNAs. hsa_circRNA_104101 was proved to promote the odontogenic differentiation of hDPSCs. Conclusion. This study reported 187 circRNAs that were differentially expressed in hDPSCs during odontogenic differentiation. Bioinformatic analysis of the expression data suggested that circRNA-miRNA-mRNA networks might act as a crucial mechanism for hDPSC odontogenic differentiation, providing a theoretical foundation for the study of pulp regeneration regulation by circRNAs
Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification
Get PDFBecause of its non-template addition feature, Taq DNA polymerase can catalyze one or more extra nucleotides onto the 3′ terminus of PCR products. An extra peak is observed at DYS391 locus after the PCR products stored for 4 days at 4°C. To explore the formation mechanism of this artifact, PCR primers and amplicon sequences of Y-STR loci are analyzed, furthermore, PCR products storage conditions and termination of PCR are discussed. The extra peak is a + 2 addition product, which we call excessive addition split peak (EASP). The most significant difference between EASP and the incomplete addition of adenine product is that the size of EASP is about one base larger than the true allele, and the EASP locates on the right side of the real allelic peak. The EASP cannot be eliminated by increasing loading mixture volume and conducting heat denaturation prior to electrophoresis injection. However, the EASP is not observed when the PCR is terminated with ethylenediaminetetraacetic acid or formamide. These findings suggest that formation of EASP is a result of 3′ end non-template extension by Taq DNA polymerase, rather than being the result of DNA fragment secondary structure produced under a suboptimal electrophoresis condition. In addition, the EASP formation is affected by the primer sequences and the storage conditions of PCR products
Targeting MSR1 to Facilitate Efferocytosis: A Novel Strategy for Immune Homeostasis Regulation in Irreversible Pulpitis
No full textAim: To investigate the role of macrophage-mediated efferocytosis in resolution of inflammation during irreversible pulpitis, with a focus on the functional relevance of macrophage scavenger receptor 1 (MSR1). Methods: Whole-transcriptome sequencing was performed on pulp tissue from 3 healthy individuals and 3 with pulpitis, integrated with Gene Expression Omnibus (GEO) datasets (GSE77459, GSE92681; total n = 30). After batch correction, differentially expressed genes (DEGs) were identified (|Fold Change|>1.5, P < .05) and analyzed by Gene Ontology (GO) / Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, CIBERSORT immune cell deconvolution, and machine learning. Efferocytosis activity was validated by immunofluorescence, Quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot (WB). MSR1 was selected for in vivo validation via small interfering RNA (siRNA) knockdown in a rat pulpitis model. Results: A total of 467 differentially expressed genes were identified, which were enriched in immune response and phagosome-related pathways. Macrophage infiltration was significantly increased in pulpitis tissues, accompanied by upregulation of efferocytosis markers. Immunofluorescence showed that MER proto-oncogene tyrosine kinase (MERTK)-positive macrophages in human inflammatory dental pulp could phagocytize apoptotic cells positive for caspase 3 (CASP3) and poly(ADP-ribose) polymerase (PARP). MSR1 is regarded as a key regulatory factor. Knockdown of Msr1 in rats can impair the clearance of apoptotic cells, reduce the expression of Mertk, and aggravate inflammation. Conclusion: MSR1 maintains immune homeostasis in the dental pulp by promoting macrophage efferocytosis, providing a theoretical basis for targeted vital pulp therapy
Dental pulp stem cells from human teeth with deep caries displayed an enhanced angiogenesis potential in vitro
No full textBackground/purpose: Dental pulp stem cells can be isolated from human teeth with deep caries (cDPSCs), but their biological characteristics are still unclear. The aim of this study was to investigate the angiogenic potential of cDPSCs and compare them to dental pulp stem cells from human normal teeth (nDPSCs). Materials and methods: Cells were isolated from human pulp tissue of normal and infected teeth with deep caries. Basic mesenchymal stem cell (MSC) characterization was conducted. Colony forming units and proliferation ability were evaluated in nDPSCs and cDPSCs. Expression of VEGF in both tissues and cells was examined by immunohistochemical staining. After stimulating nDPSCs and cDPSCs with an angiogenic medium, angiogenic markers were evaluated by qRT-PCR and western blotting. Finally, tube formation assays were used to evaluate the in vitro angiogenesis potential of both cell populations. Results: Both nDPSCs and cDPSCs possessed typical MSC characteristics. cDPSCs had enhanced colony formation and proliferation capacities than nDPSCs did. The expression of VEGF was higher in pulp tissue from teeth with deep caries and cDPSCs than in normal tissue and nDPSCs. When both cell types were grown in vitro under angiogenic conditions, cDPSCs expressed a higher level of angiogenic markers and showed a stronger angiogenesis potential than nDPSCs did. Conclusion: cDPSCs maintained MSC traits and presented a higher angiogenesis potential than nDPSCs
Table3_Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification.XLSX
No full textBecause of its non-template addition feature, Taq DNA polymerase can catalyze one or more extra nucleotides onto the 3′ terminus of PCR products. An extra peak is observed at DYS391 locus after the PCR products stored for 4 days at 4°C. To explore the formation mechanism of this artifact, PCR primers and amplicon sequences of Y-STR loci are analyzed, furthermore, PCR products storage conditions and termination of PCR are discussed. The extra peak is a + 2 addition product, which we call excessive addition split peak (EASP). The most significant difference between EASP and the incomplete addition of adenine product is that the size of EASP is about one base larger than the true allele, and the EASP locates on the right side of the real allelic peak. The EASP cannot be eliminated by increasing loading mixture volume and conducting heat denaturation prior to electrophoresis injection. However, the EASP is not observed when the PCR is terminated with ethylenediaminetetraacetic acid or formamide. These findings suggest that formation of EASP is a result of 3′ end non-template extension by Taq DNA polymerase, rather than being the result of DNA fragment secondary structure produced under a suboptimal electrophoresis condition. In addition, the EASP formation is affected by the primer sequences and the storage conditions of PCR products.</p
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