275 research outputs found
Negative Transcriptional Modulation and Silencing of the Bi-exonic Rnf35 Gene in the Preimplantation Embryo
No full textPrevious works have indicated promiscuous transcription from the zygotic genome immediately after fertilization. The mouse Rnf35 gene is bi-exonic in structure and is transcribed in the preimplantation embryo until it is permanently silenced at the blastocyst stage of development. We have previously shown that Rnf35 transcription is positively regulated by the nuclear factor Y. Using the uniquely permissive Chinese hamster ovary-K1 cell line in transient transfection assays, we demonstrate in this work that the Rnf35 promoter was negatively modulated by a cis-cognate repressor element, designated as the downstream exon 1 repressor, or DER, residing between 72 and 95 in the untranslated exon 1 of the Rnf35 gene. Simultaneous mutagenesis of the two half-sections, DER1 and DER2, of the DER sequence was required for derepression suggesting participation of multiple proteins in the DER-dependent transcriptional repression. Electrophoretic mobility shift assays demonstrated that the 3-half of DER (DER2) was targeted by the repressor CCAAT-displacement protein (CDP)/Cux. Chromatin immunoprecipitation experiments further demonstrated in vivo CDP-DER association in the blastocyst and the 8.5- day embryo. Furthermore, the DER-dependent repression was partially relieved in vivo in co-transfection with an antisense CDP construct. Transcription of the Cdp gene was shown to first occur between the eight-cell and the blastocyst stages, correlating and possibly explaining the onset of Rnf35 silencing at the blastocyst stage. Taken together, our results suggest that the evolutionarily acquired exon 1 of Rnf35, and possibly exon 1 of other similarly structured bi-exonic early embryonic genes, contributes to transcriptional modulation and silencing in the developing mouse embryo
The members of NSARF and CAKS
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The members of NSARF (National Taiwan University Hospital Study Group of ARF) and CAKS (Taiwan Consortium for Acute Kidney Injury and Renal Diseases) are listed according to their affiliation names in alphabetical order:
[Cardinal Tien Hospital]: Kuo-Cheng Lu, MD. [Chi-Mei Medical Center Liouying]: Jian-Jhong Wang, MD. [Chi-Mei Medical Center Yongkang]: Wei-Chih Kan, MD. [China Medical University Hospital]: Chiu-Ching Huang, MD, Che-Yi Chou, MD, PhD., Ya-Fei Yang, MD. [Dalin Tzu-Chi Hospital]: Jen-Pi Tsai, MD. PhD. [Far Eastern Memorial Hospital]: Hung-Yuan Chen, MD. [Hualien Tzu Chi Hospital]: Bang-Gee Hsu, MD, PhD. [International-Harvard Statistical Consulting Company]: Fu-Chang Hu, PhD. [Kaohsiung Chang Gung Memorial Hospital]: Chien-Te Lee, MD, PhD., Jin-Bor Chen, MD., Chih-Hsiung Lee, MD, Wen-Chin Lee, MD, PhD., Lung-Chih Li, MD, PhD., Te-Chuan Chen, MD. [Kaohsiung Medical University
Chung-Ho Memorial Hospital]: Hung-Chun Chen, MD, PhD., Shang-Jyh Hwang, MD., Mei-Chuan Kuo, MD. [Kaohsiung Municipal Ta-Tung Hospital]: Hugo You-Hsien Lin, MD. [Keelung Chang Gung Memorial Hospital]: Chin-Chan Lee, MD., Chiao-Yin Sun, MD., Heng-Chih Pan, MD. [Linkou Chang Gung Memorial Hospital]: Yung-Chang Chen, MD., Ming-Yang Chang, MD, PhD., Chang-Chyi Jenq, MD., Chan-Yu Lin, MD, PhD., Chih-Hsiang Chang, MD., Tsung-Yu Tsai, MD. [Lin-Shin Hospital]: Cheng-Min Chen, MD. [Luodong Saint Mary’s Hospital]: Chih-Chung Shiao, MD. [Mackay Memorial Hospital]: Chih-Jen Wu, MD, PhD.,
Cheng-Jua Lin, MD., Pei-Chen Wu, MD. [Mackay Memorial Hospital Taitung Branch]: Feng-Chi Kuo, MD. [Min-Sheng General Hospital]: Chih-Jen Weng, MD. [National Health Research Institutes]: Li-Kwang Chen, PhD. [National Taiwan University Hospital]: Kwan-Dun Wu, MD, PhD., Tzong-Shinn Chu. MD, PhD., Shuei-Liong Lin, MD, PhD., Vin-Cent Wu, MD, PhD., Chun-Fu Lai, MD, PhD. [National Taiwan University Hospital Bei-Hu Branch]: Tai-Shuan Lai, MD, PhD. [National Taiwan University Hospital Hsin-Chu Branch]: Wei-Shun Yang, MD.
[National Taiwan University Hospital Yun-Lin Branch]: Yung-Ming Chen, MD., Tao-Min Huang, MD. [New Taipei City Hospital Sanchong Branch]: Wen-Ding Hsu, MD, MS. [Shin-Kong Wo Ho-Su Memorial Hospital]: Jyh-Gang Leu, MD, PhD., Jui-Ting Chang MD. [Sin-Ren Hospital]: Hung-Hsiang Liou, MD. [Taichung Veteran General Hospital]: Kuo-Hsiung Hsu, MD. Ming-Ju Wu, MD, PhD., Chun-Te Huang, MD. [Taichung Veteran General Hospital Chiayi Branch]: Zi-hong You, MD. [Taipei City Hospital Heping Branch]: Chao-Fu Chang, MD. [Taipei Medical University Hospital]: Tzen-Wen Chen, MD. PhD., Hsi-Hsien Chen, MD. PhD., Fan-Chi
Chang, MD. PhD., Yen-Chung Lin, MD., Mai-Szu Wu, MD., Chih-Chin Kao, MD. [Taipei Tzu Chi Hospital]: Szu-Chun Hung, MD., Ko-Lin Kuo, MD, PhD., Che-Hsiung Wu, MD. [Taipei Veterans General Hospital]: Der-Cherng Tarng, MD, PhD., Wu-Chang Yang, MD., Chih-Yu Yang, MD, PhD., Kuo-Hua Lee, MD. [Taoyuan General Hospital, Ministry of Health and Welfare]: Wei-Jie Wang, MD, PhD., Sheng-Wen Ko, MD., Jui-Hsiang Lin, MD.
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Method for Expressing Multiple Recombinant Proteins in the Milk of Transgenic Non-Human Mammals
No full textThe function of porcine PPAR [gamma] and dietary fish oil effect on the expression of lipid and glucose metabolism related genes
No full textInsulin regulates the expression of adiponectin and adiponectin receptors in porcine adipocytes
No full textRecombinant porcine lactoferrin expressed in the milk of transgenic mice protects neonatal mice from a lethal challenge with enterovirus type 71
No full textEvaluation of transdifferentiation from mesenchymal stem cells to neuron-like cells using microfluidic patterned co-culture system.
No full textEfficient organic DSSC sensitizers bearing an electron-deficient pyrimidine as an effective π-spacer
No full textOrganic Dyes Containing Coplanar Diphenyl-Substituted Dithienosilole Core for Efficient Dye-Sensitized Solar Cells
No full textEvaluation of Transdifferentiation from Mesenchymal Stem Cells to Neuron- Like Cells Using Microfluidic Patterned Co-Culture System
No full textWe design a microfluidic patterned co-culture system for mouse mesenchymal stem cells mMSCs and neural cells to demonstrate the paracrine effects produced by the neural cells in facilitating the transdifferentiation from mMSCs to neuron-like cells. Neural cells and mMSC are orderly patterned in the microfluidic co-culturing system without direct cell contact. This configuration provides us to calculate the percentage of neural marker transdifferentiated by mMSCs easily. We obtain higher transdifferentiated ratio of mMSC in the microfluidic co- culturing system beta III tubulin: 67%; glial fibrillary acidic protein GFAP: 86.2% as compared with the traditional transwell co-culturing system beta III tubulin: 59.8%; GFAP: 52.0%, which is similar to the spontaneous neural marker expression in the undifferentiated MSCs beta III tubulin: 47 .5%; GFAP: 60.1%. Furthermore, mMSCs expressing green fluorescent protein and neural cells expressing red fluorescent protein were also used in our co-culture system to demonstrate the rarely occurring or observed cell fusion phenomenon. The results show that the co-cultured neural cells increased the transdifferentiation efficiency of mMSCs from soluble factors secreted by neural cells
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