2,721 research outputs found
Somatic embryogenesis and plantlet regeneration of Mangifera persiciforma C.Y. Wu & T.L. Ming
Full text linkA method for somatic embryogenesis and plant regeneration from immature nucellus (30–40 days old after pollination) of Mangifera persiciforma C.Y. Wu & T.L. Ming has been developed. Browning and necrosis of the nucellar explants were effectively controlled by pre-treating in an antioxidant solution and by pre-culturing in liquid medium in a constant temperature shaker. The explants were cultured for 2 weeks in the dark at 23 ± 2°C on induction medium and then moved to the light for 4 weeks at 25 ± 2°C Pro-embryonic masses have gradually been generated from the explants. The pro-embryogenic masses were subcultured on appropriate proliferation medium in the light for 5 weeks at 25 ± 2°C, secondary embryogenesis and organogenesis was observed. Entire plantlets have been produced. Plantlets developed in 3-4 weeks, were acclimatized and transplanted to the greenhouse. Morphogenic competence was sustained for more than one year.Key words: Mangifera persiciform, nucellus, browning, pro-embryogenic masses, somatic embryogenesis, organogenesis
Extracellular matrix and clump structure formation improve the efficiency of marrow-derived mesenchymal stem cells differentiate into insulin-producing cells
No full textDiffraction of hydroelastic waves by multiple vertical circular cylinders
No full textThe diffraction problem of hydroelastic waves beneath an ice sheet by multiple bottom-mounted circular cylinders is considered. The elastic thin-plate theory is adopted to model the ice sheet, while the linearized velocity potential theory adopted for the fluid flow. The velocity potential corresponding to each cylinder is expanded into a series of eigenfunctions, and the total potential is expressed as a summation of these expansions over the entire NC number of cylinders. For each cylinder, the Green’s second identity is used outside its domain to obtain a set of linear equations. For each different cylinder, the domain used is different. NC cylinders give NC sets of coupled linear equations. Investigations are made for different arrangements of cylinders, piercing through ice sheets. Results for the wave forces on the cylinders with clamped and free conditions of the ice edge are obtained. Physical phenomena corresponding to cylinders arranged in square, in an array, in a double-array and in a staggered double array are discussed
Wave diffraction and radiation by a vertical circular cylinder standing in a three-dimensional polynya
No full textThe wave diffraction and radiation problem of a body in a polynya surrounded by an ice sheet extending to infinity is considered through a vertical circular cylinder. The ice sheet is modelled through the elastic thin-plate theory and the fluid flow through the linearized velocity potential theory. In particular, when the polynya is of the circular shape, eigenfunction expansion method is applied to the two regions below the ice sheet and the free surface respectively, and the velocity and pressure continuity conditions are imposed on the interface of the two regions. The wave motion in the polynya, the hydrodynamic coefficients as well as the exciting forces on a body located arbitrarily in the polynya are calculated. The nature of highly oscillatory behaviour of the results is investigated and their physical implications are discussed
Transplanting porcine egfp-mesenchymal stem cells derived insulin-producing cells prolonged the life span of type I diabetes mice for 4 times
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Efficient expression and purification of milk-derived antibacterial peptides in Escherichia coli via artificial oil body system
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