187 research outputs found
Supplemental Material, 20171119_Supl_fig_2 - Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography
No full textSupplemental Material, 20171119_Supl_fig_2 for Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography by Crystel M. Gijsberts, Hester M. den Ruijter, Dominique P. V. de Kleijn, Albert Huisman, Maarten ten Berg, Mark de Groot, Richard H. A. van Wijk, Folkert W. Asselbergs, Michiel Voskuil, Gerard Pasterkamp, Wouter W. van Solinge, and Imo E. Hoefer in Angiology</p
Supplemental Material, 20171119_Supl_fig_3 - Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography
No full textSupplemental Material, 20171119_Supl_fig_3 for Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography by Crystel M. Gijsberts, Hester M. den Ruijter, Dominique P. V. de Kleijn, Albert Huisman, Maarten ten Berg, Mark de Groot, Richard H. A. van Wijk, Folkert W. Asselbergs, Michiel Voskuil, Gerard Pasterkamp, Wouter W. van Solinge, and Imo E. Hoefer in Angiology</p
Supplemental Material, 20171119_Supl_fig_1 - Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography
No full textSupplemental Material, 20171119_Supl_fig_1 for Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography by Crystel M. Gijsberts, Hester M. den Ruijter, Dominique P. V. de Kleijn, Albert Huisman, Maarten ten Berg, Mark de Groot, Richard H. A. van Wijk, Folkert W. Asselbergs, Michiel Voskuil, Gerard Pasterkamp, Wouter W. van Solinge, and Imo E. Hoefer in Angiology</p
Supplementary_Material - Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography
No full textSupplementary_Material for Hematological Parameters Outperform Plasma Markers in Predicting Long-Term Mortality After Coronary Angiography by Crystel M. Gijsberts, Hester M. den Ruijter, Dominique P. V. de Kleijn, Albert Huisman, Maarten ten Berg, Mark de Groot, Richard H. A. van Wijk, Folkert W. Asselbergs, Michiel Voskuil, Gerard Pasterkamp, Wouter W. van Solinge, and Imo E. Hoefer in Angiology</p
sj-pdf-1-jcb-10.1177_0271678X231195243 - Supplemental material for A cluster of blood-based protein biomarkers associated with decreased cerebral blood flow relates to future cardiovascular events in patients with cardiovascular disease
No full textSupplemental material, sj-pdf-1-jcb-10.1177_0271678X231195243 for A cluster of blood-based protein biomarkers associated with decreased cerebral blood flow relates to future cardiovascular events in patients with cardiovascular disease by L Malin Overmars, Sanne Kuipers, Bram van Es, Jeroen de Bresser, Esther E Bron, Imo E Hoefer, Wouter W Van Solinge, L Jaap Kappelle, Matthias JP van Osch, Charlotte E Teunissen, Geert Jan Biessels, Saskia Haitjema and Heart-Brain Connection Consortium in Journal of Cerebral Blood Flow & Metabolism</p
Impaired bone healing in multitrauma patients is associated with altered leukocyte kinetics after major trauma
No full textOkan W Bastian,1 Anne Kuijer,1 Leo Koenderman,2 Rebecca K Stellato,3 Wouter W van Solinge,4 Luke PH Leenen,1 Taco J Blokhuis1 1Department of Traumatology, 2Department of Respiratory Medicine, 3Department of Biostatistics and Research Support, Julius Center, 4Department of Clinical Chemistry and Hematology, University Medical Center Utrecht, Utrecht, the Netherlands Abstract: Animal studies have shown that the systemic inflammatory response to major injury impairs bone regeneration. It remains unclear whether the systemic immune response contributes to impairment of fracture healing in multitrauma patients. It is well known that systemic inflammatory changes after major trauma affect leukocyte kinetics. We therefore retrospectively compared the cellular composition of peripheral blood during the first 2 weeks after injury between multitrauma patients with normal (n=48) and impaired (n=32) fracture healing of the tibia. The peripheral blood-count curves of leukocytes, neutrophils, monocytes, and thrombocytes differed significantly between patients with normal and impaired fracture healing during the first 2 weeks after trauma (P-values were 0.0122, 0.0083, 0.0204, and <0.0001, respectively). Mean myeloid cell counts were above reference values during the second week after injury. Our data indicate that leukocyte kinetics differ significantly between patients with normal and impaired fracture healing during the first 2 weeks after major injury. This finding suggests that the systemic immune response to major trauma can disturb tissue regeneration. Keywords: SIRS, inflammation, neutrophils, myelopoiesis, regeneratio
Exosome mimetics: a novel class of drug delivery systems
No full textSander AA Kooijmans, Pieter Vader, Susan M van Dommelen, Wouter W van Solinge, Raymond M SchiffelersDepartment of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, The NetherlandsAbstract: The identification of extracellular phospholipid vesicles as conveyors of cellular information has created excitement in the field of drug delivery. Biological therapeutics, including short interfering RNA and recombinant proteins, are prone to degradation, have limited ability to cross biological membranes, and may elicit immune responses. Therefore, delivery systems for such drugs are under intensive investigation. Exploiting extracellular vesicles as carriers for biological therapeutics is a promising strategy to overcome these issues and to achieve efficient delivery to the cytosol of target cells. Exosomes are a well studied class of extracellular vesicles known to carry proteins and nucleic acids, making them especially suitable for such strategies. However, the considerable complexity and the related high chance of off-target effects of these carriers are major barriers for translation to the clinic. Given that it is well possible that not all components of exosomes are required for their proper functioning, an alternative strategy would be to mimic these vesicles synthetically. By assembly of liposomes harboring only crucial components of natural exosomes, functional exosome mimetics may be created. The low complexity and use of well characterized components strongly increase the pharmaceutical acceptability of such systems. However, exosomal components that would be required for the assembly of functional exosome mimetics remain to be identified. This review provides insights into the composition and functional properties of exosomes, and focuses on components which could be used to enhance the drug delivery properties of exosome mimetics.Keywords: exosomes, extracellular vesicles, liposomes, drug delivery system
Co-transcription of the gastrin and cholecystokinin genes with selective translation of gastrin mRNA in a human gastric carcinoma cell line
No full textAbstractSo far, no cells have been found to synthesize both of the homologous horomones, cholecystokinin and gastrin. Northern analysis and reverse transcription PCR showed, however, that the human gastric carcinoma cell line (AGS) expresses both a gastrin mRNA or 0.7 kb and a cholecystokinin transcript of 0.8 kb. A library of sequence-specific radioimmunoassays, cleavage with processing-like enzymes and chromatography subsequently revealed that the gastrin mRNA was translated into progastrin that was constitutively secreted into the medium (45 ± 3 pmol/1). Neither procholecystokinin nor any of its processing products were detectable in cells and media. The result suggest that differentiation into gastrin- or cholecystokinin-producing cells may be regulated at the translational level. The gastric cell line. AGS, provides a model for studies of translational regulation of cell differentiation
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