1,720,975 research outputs found
Development of 3D Cell Printed Patch-Type Stem Cell Therapy for Treatment of Liver Cirrhosis
No full textLiver cirrhosis is an irreversible end-stage liver disease which has no effective therapy except for liver transplantation, but donor shortage has been a critical limitation. Stem cell injection has been regarded as an alternative to the liver transplantation; yet, there is no optimal condition for such therapy. Moreover, the efficiency of delivery is below 10%, and the efficacy is reported to be minimal or even none. Therefore, we developed a liver patch, which is a 3D cell printed patch-type stem cell therapy with liver decellularized extracellular matrix (LdECM) bioink and human bone marrow derived mesenchymal stem cell to enhance the efficacy and delivery efficiency. The liver patch showed in vitro efficacy in the inactivation of the activated hepatic stellate cell (aHSC) by paracrine effect in transwell coculture condition. For in vivo efficacy assessment, the liver patch was implanted to the pathological liver tissue of irreversible cirrhosis mouse models. The deposited endogenous collagen was significantly decreased, and aHSCs were inactivated in the liver patch delivered cirrhosis model. Consequently, the results demonstrated that the liver patch with functional LdECM bioink and stem cell would be an effective therapy for liver cirrhosis treatment.1
3D Bioprinting of Macro Hepatic Tissue Module Using Light-Activated Liver Decellularized Extracellular Matrix-Based Bioink
No full text1
3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering
No full text3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rely on homogeneous biomaterials, which has several shortcomings such as insufficient mechanical properties and recapitulation of microenvironment. In this study, we investigated the capability of skin-derived extracellular matrix (S-dECM) bioink for 3D cell printing-based skin tissue engineering. S-dECM was for the first time formulated as a printable material and retained the major ECM compositions of skin as well as favorable growth factors and cytokines. This bioink was used to print a full thickness 3D human skin model. The matured 3D cell-printed skin tissue using S-dECM bioink was stabilized with minimal shrinkage, whereas the collagen-based skin tissue was significantly contracted during in vitro tissue culture. This physical stabilization and the tissue-specific microenvironment from our bioink improved epidermal organization, dermal ECM secretion, and barrier function. We further used this bioink to print 3D prevascularized skin patch able to promote in vivo wound healing. In vivo results revealed that endothelial progenitor cells (EPC5)-laden 3D-printed skin patch together with adipose-derived stem cells (ASCs) accelerates wound closure, re-epithelization, and neovascularization as well as blood flow. We envision that the results of this paper can provide an insightful step towards the next generation source for bioink manufacturing. (C) 2018 Elsevier Ltd. All rights reserved.11Nsciescopu
Development of Mesenchymal Stem Cell Laden Patch-Type Therapy using 3D Cell-Printing Technology for Liver Cirrhosis Treatment
No full textLiver cirrhosis is an irreversible end-stage liver disease which has no effective therapy except for liver transplantation, but donor
shortage has been a critical limitation. Stem cell injection has been regarded as an alternative therapy to the liver transplantation; yet,
there is no optimal condition for such therapy. Moreover, the efficiency of delivery is below 10%, and the efficacy is reported to be
minimal or even none. Therefore, we developed a new stem cell delivery method, which is a 3D cell printed patch-type stem cell
therapy (liver patch) with liver decellularized extracellular matrix (LdECM) bioink and human bone marrow derived mesenchymal
stem cell to enhance the efficacy and delivery efficiency. The liver patch showed in vitro efficacy in the inactivation of the activated
hepatic stellate cell (aHSC) by paracrine effect in transwell coculture condition. For in vivo efficacy assessment, the liver patch was
implanted to the pathological liver tissue of irreversible cirrhosis mouse models. The deposited endogenous collagen was significantly
decreased, and aHSCs were inactivated in the liver patch delivered cirrhosis model. Consequently, the results demonstrated that the
liver patch with functional LdECM bioink and stem cell would be an effective therapy for liver cirrhosis treatment.2
3D Cell-printing of prevascularized stem cell patch for liver cirrhosis treatment
No full textLiver cirrhosis is an irreversible liver failure which has no curative therapy except liver transplantation, but it is limited by a donor shortage. Stem cell transplantation has been regarded as an alternative therapy to the liver transplantation; yet, the delivery efficiency of stem cells and the efficacy are still controversy. Therefore, we developed a novel stem cell therapy, which is a hBMMSC (human bone marrow derived mesenchymal stem cell) / EPC (endothelial progenitor cell)-laden prevascularized stem cell patch using 3D cell-printing technology with liver decellularized extracellular matrix (LdECM) bioink to enhance the efficacy and delivery efficiency of stem cell therapy. In the in vitro study, the prevascularized stem cell patch showed anti-fibrotic effect on the activated hepatic stellate cell (aHSC) by paracrine effect. For in vivo efficacy assessment, the stem cell patch was transplanted to the pathological liver tissue of irreversible cirrhosis mouse models for 4 weeks. In the stem cell patch transplanted cirrhosis model, the hBMMSCs remained much more than the stem cell injected group during implantation, and the deposited endogenous collagen was significantly decreased. Moreover, fibrosis markers were significantly decreased in the stem cell patch delivered cirrhosis model. Consequently, the results demonstrated that the stem cell patch with functional LdECM bioink and stem cell would be an effective therapy for liver cirrhosis treatment.1
Development of Liver Decellularized Extracellular Matrix Bioink for Three-Dimensional Cell Printing-Based Liver Tissue Engineering
No full textThe liver is an important organ and plays major roles in the human body. Because of the lack of liver donors after liver failure and drug-induced liver injury, much research has focused on developing liver alternatives and liver in vitro models for transplantation and drug screening. Although numerous studies have been conducted, these systems cannot faithfully mimic the complexity of the liver. Recently, three-dimensional (3D) cell printing technology has emerged as one of a number of innovative technologies that may help to overcome this limitation. However, a great deal of work in developing biomaterials optimized for 3D cell printing-based liver tissue engineering remains. Therefore, in this work, we developed a liver decellularized extracellular matrix (dECM) bioink for 3D cell printing applications and evaluated its characteristics. The liver dECM bioink retained the major ECM components of the liver while cellular components were effectively removed and further exhibited suitable and adjustable propertes for 3D cell printing. We further studied printing parameters with the liver dECM bioink to verify the versatility and fidelity of the printing process. Stem cell differentiation and HepG2 cell functions in the liver dECM bioink in comparison to those of commercial collagen bioink were also evaluated, and the liver dECM bioink was found to induce stem cell differentiation and enhance HepG2 cell function. Consequently, the results demonstrate that the proposed liver dECM bioink is a promising bioink candidate for 3D cell printingbased liver tissue engineering.1123sciescopu
- …
