3,219 research outputs found
Epigenetic inactivation of miR-9 family microRNAs in chronic lymphocytic leukemia--implications on constitutive activation of NFκB pathway.
No full textBackground: The miR-9 family microRNAs have been identified as a tumor suppressor miRNA in cancers. We postulated that miR-9-1, miR-9-2 and miR-9-3 might be inactivated by DNA hypermethylation in chronic lymphocytic leukemia (CLL).Methods: Methylation of miR-9-1, miR-9-2 and miR-9-3 was studied in eight normal controls including normal bone marrow, buffy coat, and CD19-sorted peripheral blood B-cells from healthy individuals, seven CLL cell lines, and seventy-eight diagnostic CLL samples by methylation-specific polymerase chain reaction.Results: The promoters of miR-9-3 and miR-9-1 were both unmethylated in normal controls, but methylated in five (71.4%) and one of seven CLL cell lines respectively. However, miR-9-2 promoter was methylated in normal controls including CD19 + ve B-cells, hence suggestive of a tissue-specific but not tumor-specific methylation, and thus not further studied. Different MSP statuses of miR-9-3, including complete methylation, partial methylation, and complete unmethylation, were verified by quantitative bisulfite methylation analysis. 5-Aza-2′-deoxycytidine treatment resulted in miR-9-3 promoter demethylation and re-expression of pri-miR-9-3 in I83-E95 and WAC3CD5+ cells, which were homozygously methylated for miR-9-3. Moreover, overexpression of miR-9 led to suppressed cell proliferation and enhanced apoptosis together with downregulation of NFκB1 in I83-E95 cells, supporting a tumor suppressor role of miR-9-3 in CLL. In primary CLL samples, miR-9-3 was detected in 17% and miR-9-1 methylation in none of the patients at diagnosis. Moreover, miR-9-3 methylation was associated with advanced Rai stage (≥ stage 2) (P = 0.04).Conclusions: Of the miR-9 family, miR-9-3 is a tumor suppressor miRNA relatively frequently methylated, and hence silenced in CLL; whereas miR-9-1 methylation is rare in CLL. The role of miR-9-3 methylation in the constitutive activation of NFκB signaling pathway in CLL warrants further study
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Influence of diluent alkyl substitution on the extraction of Am(III) and Eu(III) by a 6,6’-bis(1,2,4-triazin-3-yl)-2,2’-bipyridine ligand dissolved in alkylated cyclohexanone Diluents
No full textSeveral alkylated cyclohexanones were investigated as potential diluents for the selective extraction of Am(III) from Eu(III) from nitric acid solutions by the CyMe4-BTBP ligand. No significant extraction of either of the metal ions was observed for these diluents themselves. In the extractions from 1 M HNO3, 3-methylcyclohexanone and 4-methylcyclohexanone gave comparable results to cyclohexanone whereas in the extractions from 4 M HNO3, 2-methylcyclohexanone, 3-methylcyclohexanone and 4-methylcyclohexanone all gave superior results. For the monomethylated diluents, DAm and SFAm/Eu decreased in the order of alkyl substitution 2 > 4 ~ 3. However, alkyl substitution of cyclohexanone significantly slows down the extraction kinetics compared to cyclohexanone, and the position of alkyl substitution was found to play an important role in the solvents properties. 3-Methylcyclohexanone was identified as the most promising of the diluent
sj-docx-1-pom-10.1177_03057356231159194 – Supplemental material for A cognitive intervention to correct a maladaptive technique in organists due to prior music learning: A randomized controlled trial
No full textSupplemental material, sj-docx-1-pom-10.1177_03057356231159194 for A cognitive intervention to correct a maladaptive technique in organists due to prior music learning: A randomized controlled trial by Mandy MP Kan, Nicola Dibben and Arnold YL Wong in Psychology of Music</p
Rapid and Efficient Synthesis of 1<i>H</i>-Indol-2-yl-1<i>H</i>-quinolin-2-ones
No full textA concise and efficient synthesis of the novel indol-2-yl-1H-quinolin-2-one ring system found in the potent and selective KDR kinase inhibitors
1−3 is presented
Utilizing the principles of Gagne’s nine events of instruction in the teaching of Goldmann Applanation Tonometry
No full textYee Ling Wong Ophthalmology Department, Ninewells Hospital and Medical School, Dundee, UK Abstract: Intraocular pressure measurement is important to identify people who are at risk of glaucoma. Goldmann Applanation Tonometry is considered the gold standard with regards to evaluating patients with glaucoma, a sight-threatening condition. The professional execution of this evaluation is critical in the reduction of associated complication rates. This article seeks to put forward a structured lesson plan for ophthalmology trainees, specialist nurses, and optometrists, which integrates Gagne’s nine events of instruction to accomplish this objective. Keywords: IOP, Gagne’s model, lesson plan, Peyton’s steps, glaucoma, ophthalmology teachin
sj-pdf-1-pmj-10.1177_02692163211049309 – Supplemental material for Pain assessment tools in paediatric palliative care: A systematic review of psychometric properties and recommendations for clinical practice
No full textSupplemental material, sj-pdf-1-pmj-10.1177_02692163211049309 for Pain assessment tools in paediatric palliative care: A systematic review of psychometric properties and recommendations for clinical practice by Adrienne YL Chan, Mengqin Ge, Emily Harrop, Margaret Johnson, Kate Oulton, Simon S Skene, Ian CK Wong, Liz Jamieson, Richard F Howard and Christina Liossi in Palliative Medicine</p
sj-pdf-2-pmj-10.1177_02692163211049309 – Supplemental material for Pain assessment tools in paediatric palliative care: A systematic review of psychometric properties and recommendations for clinical practice
No full textSupplemental material, sj-pdf-2-pmj-10.1177_02692163211049309 for Pain assessment tools in paediatric palliative care: A systematic review of psychometric properties and recommendations for clinical practice by Adrienne YL Chan, Mengqin Ge, Emily Harrop, Margaret Johnson, Kate Oulton, Simon S Skene, Ian CK Wong, Liz Jamieson, Richard F Howard and Christina Liossi in Palliative Medicine</p
A novel right-angled ligand that forms polymeric metal-organic frameworks with nanometer-sized square cavities
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