135 research outputs found
The Tree Project - Life & Legacy of Dorothy Hogg: Misun Won and Andrew Lamb [podcast]
No full textDuring her time as Head of Jewellery & Silversmithing at Edinburgh College of Art, renowned jeweller and educator Dorothy Hogg MBE inspired students in the workshop and beyond. Over a series of Interviews, alumni of the programme during Dorothy's tenure between 1985 and 2008 reflect on the lasting impact of her teaching throughout their diverse career paths. With humour and illustrative memories, these stories bring to life the world of the workshop at Edinburgh College of Art
Data on the transcriptional regulation of DNA damage induced apoptosis suppressor (DDIAS) by ERK5/MEF2B pathway in lung cancer cells
No full textAbstractThe data included in this article are associated with the article entitled “DNA-damage-induced apoptosis suppressor (DDIAS) is upregulated via ERK5/MEF2B signaling and promotes β-catenin-mediated invasion” (J.Y. Im, S.H. Yoon, B.K. Kim, H.S. Ban, K.J. Won, K.S. Chung, K.E. Jung, M. Won) [1]. Quantitative RT-PCR data revealed that genetic or pharmacological inhibition of extracellular signal-regulated kinase 5 (ERK5) suppresses DDIAS transcription in response to epidermal growth factor (EGF) in Hela cells. p300 did not interact with myocyte enhancer factor 2B (MEF2B), a downstream target of ERK5 and affect transcription of DDIAS. Moreover, DDIAS transcription is activated by ERK5/MEF2B signaling on EGF exposure in the non-small cell lung cancer cells (NSCLC) NCI-H1703 and NCI-H1299. DDIAS knockdown suppresses lung cancer cell invasion by decreasing β-catenin protein level on EGF exposure
AsiDesigner: exon-based siRNA design server considering alternative splicing
No full textRNA interference (RNAi) with small interfering RNA (siRNA) has become a powerful tool in functional and medical genomic research through directed post-transcriptional gene silencing. In order to apply RNAi technique for eukaryotic organisms, where frequent alternative splicing results in diversification of mRNAs and finally of proteins, we need spliced mRNA isoform silencing to study the function of individual proteins. AsiDesigner is a web-based siRNA design software system, which provides siRNA design capability to account for alternative splicing for mRNA level gene silencing. It provides numerous novel functions including the designing of common siRNAs for the silencing of more than two mRNAs simultaneously, a scoring scheme to evaluate the performance of designed siRNAs by adopting currently known key design factors, a stepwise off-target searching with BLAST and FASTA algorithms and checking the folding secondary structure energy of siRNAs. To do this, we developed a novel algorithm to evaluate the common target region, where siRNAs can be designed to knockdown a specific mRNA isoform or more than two mRNA isoforms from a target gene simultaneously. The developed algorithm and the AsiDesigner were tested and validated as very effective throughout widely performed gene silencing experiments. It is expected that AsiDesigner will play an important role in functional genomics, drug discovery and other molecular biological research.open
An Examination of Sport Ticket Price Acceptability and Surcharge Transparency in Partitioned Pricing
No full textThere has been a recent price policy change in the sport industry that ticket resale companies attempted to reveal any additional fees upfront to increase price transparency and protect consumers in the marketplace. This policy change was announced in early 2020 (Thompson, 2020). However, as the coronavirus outbreak affected live events to be canceled, become virtual, or have a limited facility capacity (Apstein, 2020; Perry, 2020), it disabled the resale companies to see consumer responses to their policy change that may increase or decrease ticket revenues. In addition, charging additional fees to the ticket face value is a form of partitioned pricing and drip pricing, which contains two price components: a base price and surcharges (Burman & Biswas, 2007; Morwitz et al., 1998). This means that purchase decisions may vary depending on whether the base price or the total cost of tickets is below (or above) the price range individuals consider acceptable. This makes an examination of price acceptability within partitioned pricing and drip pricing imperative in terms of understanding consumer purchase decisions. Therefore, this particular study aimed to disclose consumer perceptions (i.e., surcharge sensitivity, surcharge acceptability, surcharge skepticism) and purchase behaviors (i.e., search intention, purchase intention) regarding surcharge transparency. An experimental between-participants design with four groups (no fees vs. transparent fees vs. a notification of fees vs. hidden fees) was used to manipulate surcharge transparency that is currently employed on the secondary market by various companies. An online survey was developed on Qualtrics, and data from a total of 547 participants was collected on Amazon Mechanical Turk. The author employed four multivariate analyses of covariances for data analysis. This study found that, first, when ticket prices are below individuals’ acceptable price, they have high intention to purchase the ticket. The opposing result occurred when ticket prices exceed individuals’ threshold. However, consumers consistently have high search intention regardless of price acceptability. Second, due to sport consumers’ acknowledgment that additional surcharges are added to ticket prices when purchasing them on the secondary market, the way surcharges are presented does not vary their surcharge perceptions. Rather, the size of surcharges (e.g., $2.50 vs. 25% of the base price) differs surcharge perceptions. The acknowledgement of estimated fees on the secondary market makes the effects of surcharge transparency insignificant on purchase behavior as well as the moderating impacts of surcharge perceptions. This study makes contributions to the PP literature and the sport consumer behavior literature. The findings contribute to providing a comprehensive understanding of consumer behavior with two common surcharges in live ticket purchases. This study particularly advances the literature with fundamental moderators (e.g., price acceptability, surcharge transparency) and essential outcome variables (e.g., search intention, purchase intention) within the context of sports. In addition, the present study is guided by attribution theory (Heider, 1958; Kelley & Michela, 1980); i.e., sport consumers acknowledge that surcharges exist in order to provide the ticketing service for consumers and to generate revenues for organizations. This attribution neutralizes the effects of PP on the secondary market. From the managerial standpoint, the findings of this study provide resale companies with effective price presentation styles. In order to enhance sales revenue, companies are recommended to employ all-inclusive pricing (no price breakdowns). However, companies should ensure they clearly communicate any fees that are included in the total price in order to increase price transparency
UNC93B1 is essential for the plasma membrane localization and signaling of Toll-like receptor 5
No full textThe proper trafficking and localization of Toll-like receptors (TLRs) are important for specific ligand recognition and efficient signal transduction. The TLRs sensing bacterial membrane components are expressed on the cell surface and recruit signaling adaptors to the plasma membrane upon stimulation. On the contrary, the nucleotide-sensing TLRs are mostly found inside cells and signal from the endolysosomes in an acidic pH-dependent manner. Trafficking of the nucleotide-sensing TLRs from the endoplasmic reticulum to the endolysosomes strictly depends on UNC93B1, and their signaling is completely abolished in the 3d mutant mice bearing the H412R mutation of UNC93B1. In contrast, UNC93B1 was considered to have no role for the cell surface-localized TLRs and signaling via TLR1, TLR2, TLR4, and TLR6 is normal in the 3d mice. Unexpectedly, we discovered that TLR5, a cell surface receptor for bacterial protein flagellin, also requires UNC93B1 for plasma membrane localization and signaling. TLR5 physically interacts with UNC93B1, and the cells from the 3d or UNC93B1-deficient mice not only lack TLR5 at the plasma membrane but also fail to secret cytokines and to up-regulate costimulatory molecules upon flagellin stimulation, demonstrating the essential role of UNC93B1 in TLR5 signaling. Our study reveals that the role of UNC93B1 is not limited to the TLRs signaling from the endolysosomes and compels the further probing of the mechanisms underlying the UNC93B1-assisted differential targeting of TLRs.
Hypoxia-inducible factor (HIF) inhibitors: a patent survey (2011-2015)
No full textIntroduction: Hypoxia-inducible factor (HIF)-1α regulates the expression of genes involved in angiogenesis, cellular energy metabolism, and cell survival during cancer development. The increased expression of HIF-1α in most solid tumors is associated with poor prognoses and therapeutic outcomes. Therefore, HIF has been recognized as an attractive target for cancer therapy, and many HIF inhibitors have been reported. Areas covered: This patent survey summarizes the information about patented HIF inhibitors over the last 5 years (2011-2015). Expert opinion: Although many of the HIF inhibitors reviewed in this patent survey possess inhibitory activity against cancer and HIF-related diseases, the compounds are still in the early stages of development, most likely due to the complexity of the HIF-1 pathway and their different mechanisms of action for HIF inhibition. Most cancer cells use the glycolytic pathway for energy production and HIF-1α participates deeply in the expression of several glycolytic enzymes. Therefore, a detailed study of HIFs function in cancer metabolisms may provide us an alternative strategy for further development of HIF inhibitors in cancer therapy.open
A Concise Dictionary of Transparency
No full textThe article discusses an essay collection by Marek Bieńczyk, "Przeźroczystość"[Transparency]. The concept, placed in various context, shows various aspects and is seen in various shades. The author does not put forward a statement, but rather proposes a work to be done: to determine the modality of transparency. The concept initially seems to be mainly epistemological: the cognizant subject would like to make the world transparent, to discover all possible mysteries. Before that, however, the subject must know itself, and here the dream of transparency also plays the key role. Lack of epistemological transparency is the main cause of melancholy and its reverse — hysteria. The concept turns out to be important in the domain of love — the loer thinks that (s)he knows the desired person more better anybody else, that (s)he has entirely penetrated the subjectivity of the Other. Ultimately, however, the dream of transparency goes down to a slow demise of the subject: as self-discovery progresses, there is less and less of the discoverer. In conclusion of this work, the border of modality of the concept turns out to be horrifyingly obvious. The desire for transparency consequently searches not for knowledge, but for an escape whose name is death.21239252Poznańskie Studia Polonistyczne. Seria Literack
Abstract 1164: A novel hypoxia-inducible factor-1 inhibitor IDF-11774 regulates cancer metabolism, thereby suppressing tumor growth
No full textAbstract
HIF-1 is associated with poor patient prognosis and therapeutic resistance of cancer. We have developed a novel hypoxia-inducible factor (HIF)-1 inhibitor, IDF-11774, as a clinical candidate for cancer therapy. Under hypoxic condition, IDF-11774 inhibited the accumulation of HIF-1α in vitro and in vivo in colorectal carcinoma HCT116 cells. IDF-11774 suppressed the angiogenesis of cancer cells by reducing the expression of HIF-1 target genes. Moreover, IDF-11774 reduced glucose uptake, leading sensitizing cell growth on low glucose condition. IDF-11774 also decreased the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR). Metabolic profile of IDF-11774-treated cells revealed low levels of NAD+, lactate, and intermediates in glycolysis and tricarboxylic acid (TCA) cycle. More importantly, we observed elevated AMP and diminished ATP level, leading high AMP/ATP ratio. Apparently, phosphorylation of AMPK increased, leading inhibition of mTOR signaling in cells. In vivo xenograft assays demonstrated that IDF11774 has significant anti-cancer efficacy by targeting cancer metabolism in mouse models containing the KRAS, PTEN or VHL mutation, which often occurs in many malignant cancers. Collectively, IDF-11774 suppresses the hypoxia-induced HIF-1α accumulation, thereby repressing tumor growth by regulating cancer metabolism.
Citation Format: Misun Won, Hyun Seung Ban, Kyeong Lee, Hongsub Lee, Bo-Kyung Kim, Hwan Mook Kim, Ravi Naik, Song-Kyu Park, Joon-Tae Park, Inhyub Kim, Miso Nam, Geum-Sook Hwang. A novel hypoxia-inducible factor-1 inhibitor IDF-11774 regulates cancer metabolism, thereby suppressing tumor growth [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 1164. doi:10.1158/1538-7445.AM2017-1164</jats:p
'Linear Brooch' included in the Interwoven: Jewellery Meets Textiles Exhibition
No full textThe interconnection of jewellery and textiles has a long history, given the intimate relationship of both crafts to the body and personal expression. But what is the nature of this bond today? Looking from the perspective of the contemporary jeweller, the catalogue and exhibition ask how and why are makers looking to textiles as they design?
Jewellers featured are: Flora Bhattachary, Eleanor Bolton, Elizabeth Bone, Caroline Broadhead, Megan Brown, Nina Bukvic, Susan Cross, Alison Evans, Ella Fearon-Low, Nora Fok, Gill Galloway-Whitehead, Anna Gordon, Faye Hall, Sue Hiley Harris, Teri Howes, Yoko Izawa, Ulli Kaiser, Andrew Lamb, Gilly Langton, Ruth Leslie, Catherine Martin, Jacqueline Mina, Caitlin Murphy, Angela O'Kelly, David Poston, Sarah Pulvertaft, Jane Sedgwick, Charlotte Smith, Joanne Thompson, Liz Willis, Misun Won
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