1,354,676 research outputs found
Windmill Islands flying bird nesting areas and adelie penguin colonies from field work by Eric Woehler
No full textProgress Code: completedStatement: Each polygon feature has a Qinfo value which can be used to access data quality information (see provided URL). Eric captured the nest locations using handheld GPS.This GIS dataset is the result of field work in the Windmill Islands by Eric Woehler. <br/>The locations are Frazier Islands, Shirley Island, Ford Island and Casey station. <br/>Polygon data represents the extents of flying bird nesting areas and adelie penguin colonies. Point data represents nest locations
Woehler and Force
No full textStreet view of the front of the Woehler and Force store. A portion of the house next to the building is visible along with a building behind the store. There is a sign on a pillar that reads, 'Kaiser Frazer.' There are two other signs above the windows that read, 'Farm Equipment,' and 'Woehler & Force.'; Verso There is a sticker with typed black ink reading, 'Woehler & Force 1316-22 8th Ave. - Greeley, CO 1947. Orig. env. says Liberty Trucker Parts Co.
Simultaneous quantitative live cell imaging of multiple FRET-based biosensors.
No full textWe have developed a novel method for multi-color spectral FRET analysis which is used to study a system of three independent FRET-based molecular sensors composed of the combinations of only three fluorescent proteins. This method is made possible by a novel routine for computing the 3-D excitation/emission spectral fingerprint of FRET from reference measurements of the donor and acceptor alone. By unmixing the 3D spectrum of the FRET sample, the total relative concentrations of the fluorophores and their scaled FRET efficiencies are directly measured, from which apparent FRET efficiencies can be computed. If the FRET sample is composed of intramolecular FRET sensors it is possible to determine the total relative concentration of the sensors and then estimate absolute FRET efficiency of each sensor. Using multiple tandem constructs with fixed FRET efficiency as well as FRET-based calcium sensors with novel fluorescent protein combinations we demonstrate that the computed FRET efficiencies are accurate and changes in these quantities occur without crosstalk. We provide an example of this method's potential by demonstrating simultaneous imaging of spatially colocalized changes in [Ca(2+)], [cAMP], and PKA activity
Woehler and Force Farm Equipment
No full textStreet view of the front exterior of Woehler and Force Farm Equipment. Several automobiles are visible through the windows of the store. The alley along the side of the building is also visible. There are several signs along the front of the building reading, 'Farm Equipment,' 'Kaiser Frazer W&F,' and 'Woehler & Force.'; Verso There is a sticker with typed black ink reading, 'Woehler & Force 1316-22 8th Ave. - Greeley, CO 1947. Orig. env. says Liberty Trucker Parts Co./ 690 Lincoln St./F.V. Altwater/POB 1889/Denver, Colo.
Woehler and Force Farm Equipment garage
No full textInterior of a garage at Woehler and Force Farm Equipment. There are two automobiles parked in the garage and several of the doors are open. Various equipment is visible on tables in the room.; Verso There is a sticker with typed black ink reading, 'Woehler & Force Farm Equipment 1316-22 8th Ave. - Greeley, CO 1947. Interior view. Garage. A Kaiser-Frazer dealer.
Fatigue testing results shown in a Woehler graph.
No full text<p>Fatigue testing results shown in a Woehler graph.</p
Woehler and Force Farm Equipment show room
No full textShow room at Woehler and Force Farm Equipment. There is a truck in the room with a man standing next to it and another man sitting next to it. There are banners and posters around the room promoting various farm equipment related products.; Verso There is a sticker with typed black ink that reads, 'Woehler & Force - Interior 1316-22 8th Ave. - Greeley, CO 1947. Orig. env. says Liberty Trucker Parts Co./ 690 Lincoln St. /F.V. Altwater/POB 1889/ Denver, Colo.
Woehler and Force Farm Equipment garage
No full textInterior of a garage at Woehler and Force Farm Equipment. There are three automobiles parked in the garage and several of the doors are open. Various equipment is visible on tables in the room.; Verso There is a sticker with typed black ink reading, 'Woehler & Force - Interior 1316-22 8th Ave. - Greeley, CO 1947. Orig.env. says Liberty Trucker parts Co./690 Lincoln St./F.V. Altwater/POB 1889/Denver, Colo.
Woehler and Force Farm Equipment show room
No full textA truck and four men in a show room at Woehler and Force Farm Equipment. One of the men is sitting in a chair, another is next to the truck, one is behind a counter and one is leaning against the counter. There are various posters and displays around the room relating to farm equipment and automobiles.; Verso There is a sticker with typed black ink reading, 'Woehler & Force Farm Equipment 1316-22 8th Ave. - Greeley, CO 1947. Interior view. Sales floor A Kaiser-Frazer dealer.
Quantitative Intensity-Based FRET Approaches—A Comparative Snapshot
No full textAbstractFörster resonance energy transfer (FRET) has become an important tool for analyzing different aspects of interactions among biological macromolecules in their native environments. FRET analysis has also been successfully applied to study the spatiotemporal regulation of various cellular processes using genetically encoded FRET-based biosensors. A variety of procedures have been described for measuring FRET efficiency or the relative abundance of donor-acceptor complexes, based on analysis of the donor fluorescence lifetime or the spectrally resolved fluorescence intensity. The latter methods are preferable if one wants to not only quantify the apparent FRET efficiencies but also calculate donor-acceptor stoichiometry and observe fast dynamic changes in the interactions among donor and acceptor molecules in live cells. This review focuses on a comparison of the available intensity-based approaches used to measure FRET. We discuss their strengths and weaknesses in terms of FRET quantification, and provide several examples of biological applications
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