12 research outputs found

    Genotypic differences in vase life of Anthurium andraeanum (Hort.) cut-flowers are associated with differences in spathe chlorophyll content

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    Anthurium (Anthurium andraeanum (Hort.)) is an important crop in the worldwide cut-flower industry, valued in particular for its showy colors and long vase life, which varies from a few days to several weeks depending on the cultivar. Previous work suggested that long vase life is associated with the chlorophyll content of the spathe, especially when more light is available. Here we compare the vase life of ten anthurium cultivars covering a range of chlorophyll contents, using two light intensities: low light (40 µmol m −2 s −1) and high light (400 µmol m −2 s −1). The experiment was arranged in a factorial design with six replicate cut-flowers per cultivar per light intensity, and measurements were recorded weekly for cut-flower degradation, water uptake, spathe chlorophyll content and spathe hyperspectral reflectance. Cultivar differences in vase life were positively associated with differences in the initial spathe chlorophyll content (accounting for &gt; 60 % of the variation). The use of high light also significantly increased vase life in three of the high chlorophyll cultivars. Chlorophyll content increased over time in some cultivars, and this increase was associated with extended vase life under high light (accounting for 69 % of the variation). In both cases chlorophyll content provided a better indicator of vase life than the red dip index (R800 nm – R685 nm) derived from hyperspectral reflectance. The results show that cultivars with a high initial chlorophyll content often have an ability to ‘re-green’ by increasing chlorophyll content while in the vase. Both high initial chlorophyll content and the ability to perform re-greening are associated with a longer vase life suggesting that chlorophyll activity in the spathe helps to maintain the cut-flowers. </p

    The impact of light on vase life in (Anthurium andraeanum Hort.) cut flowers

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    The impact of light quality and quantity on vase life was investigated using Anthurium (Anthurium andraeanum Hort.). Cultivars ‘Spirit’ and ‘Honduras’, were chosen based on their contrasting vase life in previous studies, and designated as ‘Vshort’ and ‘Vlong’ respectively. Both cultivars were kept under three light regimes at 12 h day length: fluorescent lights of 40 μmol m-2 s-1, low intensity LEDs producing light at 40 μmol m-2 s-1 and high intensity LEDs producing light at 400 μmol m-2 s-1. Degradation, water uptake and hyperspectral reflectance were measured three times a week as the cut flowers degraded. Spadix necrosis was used to quantify cut flower degradation over time and to determine the vase life for each cultivar. Light regime had a significant impact on vase life and water uptake in Vlong but not in Vshort, with high intensity LEDs resulting in a marked increase in the vase life of Vlong. The rate of water uptake was higher for Vshort, while Vlong maintained moderate and steady water uptake over time, particularly under high intensity LEDs. The reflectance spectrums changed during spathe degradation, with different responses seen in each cultivar. Reflectance spectrums showed consistent changes in the ‘red dip index’ (R800-R685), with Vshort demonstrating an increase in reflectance of red light over time while Vlong increased its absorption of red light over time. Extension of vase life under high intensity light was cultivar-dependent, revealing a previously unknown interaction between light and vase life. The absence of any interaction in the short vase life cultivar suggests that this mechanism is linked to genotypic differences in vase life, while the contrasting reflectance profiles suggest that pigment turnover is important for regulating this mechanism

    Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies

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    Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.EEA Bella VistaFil: Behlau, Franklin. Fundo de Defesa da Citricultura. Pesquisa e Desenvolvimento; BrasilFil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Lugo, Alshia Janel. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y TobagoFil: Elibox, Winston. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y TobagoFil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados UnidosFil: Potnis, Neha . University of Florida. Department of Plant Pathology; Estados UnidosFil: White, Frank F. Kansas State University. Department of Plant Pathology; Estados UnidosFil: Ebrahim, Mohamed. University of Florida. Department of Plant Pathology; Estados Unidos. Ain Shams University. Faculty of Agriculture. Department of Plant Pathology; EgiptoFil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados UnidosFil: Ramsubhag, Adesh. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobag
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