2,893 research outputs found
Evaluating an Evolutionary Approach for Reconstructing Gene Regulatory Networks
No full textWhitehead D, Skusa A, Kennedy PJ. Evaluating an Evolutionary Approach for Reconstructing Gene Regulatory Networks. In: Proceedings of the 9th International Conference on the Simulation and Synthesis of Living Systems (ALIFE 9). 2004: 427-432
Book Discussion : PJ Powers
No full textThe UJ Campus Health Services and the Student Affairs Division in partnership with the UJ Library invite you to meet PJ Powers (Thandeka) the co-author of the book HERE I AM About the book: Here I Am, written with Marianne Thamm, is an intimate and hilarious account of the life and times of one of this country’s most recognisable and enduring performers. From the dizzying heights of international stardom to the dark depths of her struggle with alcohol, this is a must-read to explore the heady mix of politics and music of the time. More than just a story about the personal journey of one of South Africa’s most beloved music icons, this extraordinary memoir of PJ Powers – or Thandeka, as she was affectionately renamed by Soweto crowds – is set against the turbulent backdrop of South Africa’s recent political history. It features a gallery of political leaders and international celebrities, including the likes of Nelson Mandela, Graça Machel, Chris Hani, Joaquim Chissano, Queen Elizabeth II, Brenda Fassie, Sharon Stone and Robert De Niro. Facilitator: Prof Alban Burke, Director – PsyCad, University of Johannesburg PJ Powers will also perform a few songs on the day. Date: 27 August 2015 Time: 16:30 for 17:00 Venue: Auditorium (6th Floor), APK Library, University of Johannesburg (corner Kingsway and University Road, Auckland Park) RSVP: By Wednesday, 26 August 2015 to Theodorah Modise on [email protected] / 011 559 226
Book Discussion : PJ Powers
No full textThe UJ Campus Health Services and the Student Affairs Division in partnership with the UJ Library invite you to meet PJ Powers (Thandeka) the co-author of the book HERE I AM About the book: Here I Am, written with Marianne Thamm, is an intimate and hilarious account of the life and times of one of this country’s most recognisable and enduring performers. From the dizzying heights of international stardom to the dark depths of her struggle with alcohol, this is a must-read to explore the heady mix of politics and music of the time. More than just a story about the personal journey of one of South Africa’s most beloved music icons, this extraordinary memoir of PJ Powers – or Thandeka, as she was affectionately renamed by Soweto crowds – is set against the turbulent backdrop of South Africa’s recent political history. It features a gallery of political leaders and international celebrities, including the likes of Nelson Mandela, Graça Machel, Chris Hani, Joaquim Chissano, Queen Elizabeth II, Brenda Fassie, Sharon Stone and Robert De Niro. Facilitator: Prof Alban Burke, Director – PsyCad, University of Johannesburg PJ Powers will also perform a few songs on the day. Date: 27 August 2015 Time: 16:30 for 17:00 Venue: Auditorium (6th Floor), APK Library, University of Johannesburg (corner Kingsway and University Road, Auckland Park) RSVP: By Wednesday, 26 August 2015 to Theodorah Modise on [email protected] / 011 559 226
An automated data-driven pipeline for improving heterologous enzyme expression
No full textA key challenge in cellular biomanufacturing of fuels, chemicals, and pharmaceuticals is that many pathway enzymes have very low activity, limiting overall titers and productivities. One reason is that enzymes are marginally stable under their native conditions, and expression in a different environment can thermodynamically favor the unfolded state. Additionally, overexpression can result in aggregation because natively expressed proteins are close to their solubility limit. This challenge suggests an engineering solution: engineer pathways enzymes to be stable in their biomanufacturing chassis. However, this is difficult because: (a.) many enzymes do not have high-throughput activity screens needed for directed evolution; (b.) there are few or no structures available; (c.) there are often multiple limiting enzymes; (d.) most mutations confer small benefits to stability; and (e.) the plurality of stability-enhancing mutations decrease catalytic efficiency. I will present a culmination of my group\u27s approach to solve the above challenges, in effect automating the design of stable, active enzymes from limited combinatorial datasets. This engineering strategy involves user-defined precise mutagenesis1,2, deep sequencing to evaluate the functional effect of nearly all possible single point mutants on solubility3, Bayesian methods to discriminate stable, catalytically neutral from deleterious mutations3, and computational design to combine up to 50 mutations at once4. I will show recently published work on application of this method to improve the pathway productivity of a medicinal alkaloid pathway in Saccharomyces cerevisiae5, and end with the description of a computational pipeline to automate our process for any enzyme of interest. References 1. Wrenbeck EE, KlesmithJR, AdeniranA, StapletonJA, Tyo KJ, Whitehead TA, (2016) Plasmid-based single-pot saturation mutagenesis, Nature Methods 13(11): 928-930 doi:10.1038/nmeth.4029 2. Medina-Cucurella A, Steiner PJ, Faber MS, Beltran J, Borelli A, Kirby M, Cutler S, Whitehead TA, (2019) “User-defined single pot mutagenesis using unpurified oligo pools”, under review 3. Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA (2017) Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning, PNAS 114:2265-2270 doi: 10.1073/pnas.1614437114 4. Klesmith JR, Bacik JP, Michalczyk R, Whitehead TA (2015) High-resolution sequence function mapping of a levoglucosan utilization pathway in E. coliâ, ACS Synthetic Biology 4 (11), 1235-1243 DOI: 10.1021/acssynbio.5b001 5. Wrenbeck EE, Bedewitz MA, Klesmith JR, Noshin S, Barry CS, Whitehead TA* (2019) “An automated data-driven pipeline for improving heterologous enzyme expression” ACS Synthetic Biology accepte
Canonical triangulations of Dehn fillings
No full textEvery cusped, finite-volume hyperbolic three-manifold has a canonical decomposition into ideal polyhedra. We study the canonical decomposition of the hyperbolic manifold obtained by filling some (but not all) of the cusps with solid tori: in a broad range of cases, generic in an appropriate sense, this decomposition can be predicted from that of the unfilled manifold (a similar result has been independently announced by Akiyoshi [4]). We also find the canonical decompositions of all hyperbolic Dehn fillings on one cusp of the Whitehead link complement
Technologically mediated learning: The future of training in Australia
No full textHosie, PJ ORCiD: 0000-0003-2585-024XFollowing a review of the economic imperatives currently facing Australia,
the future directions training will take are examined. Related training issues are considered; such as multiskilling, on-the-job training and legal issues. The author predicts that technologically mediated learning (TML),
especially interactive multimedia, will gain ascendancy as the predominant mode of delivery for training
A 0.7-V 0.43-pJ/cycle Wakeup Timer based on a Bang-bang Digital-Intensive frequency-Locked-Loop for IoT Applications
No full textA 40-nm CMOS wakeup timer employing a bang-bang digital-intensive frequency-locked loop for Internet-of-Things applications is presented. A self-biased ΣΔ digitally controlled oscillator (DCO) is locked to an RC time constant via a single-bit chopped comparator and a digital loop filter. Such highly digitized architecture fully exploits the advantages of advanced CMOS processes, thus enabling operation down to 0.7 V and a small area (0.07 mm 2 ). Most circuitry operates at 32× lower frequency than the DCO in order to reduce the total power consumption down to 181 nW. High frequency accuracy and a 10× enhancement of long-term stability is achieved by the adoption of chopping to reduce the effect of comparator offset and 1/f noise and by the use of ΣΔ modulation to improve the DCO resolution. The proposed timer achieves the best energy efficiency (0.43 pJ/cycle at 417 kHz) over prior art while keeping excellent on-par long-term stability (Allan deviation floor <;20 ppm) and temperature stability (106 ppm/°C).Accepted Author Manuscript(OLD)Applied Quantum Architecture
EFEKTIVITAS PASAL 1 PERATURAN DIREKTUR JENDRAL PAJAK NOMOR PER-18/PJ/2017
No full textTahap Penelitian atau yang penulis biasa sebut dengan validasi SSP (Surat Setoran Pajak) merupakan tahap final dimana seorang wajib pajak telah melakukan kewajibannya dalam penyetoran pajak, dan dalam proses validasi itu sendiri tidak semua akan diterima oleh kantor pajak setempat karena masih akan dilakukan penelitian baik penelitian serara formil maupun materiil. Untuk mekanismenya validasi telah ditetapkan dalam Per-18/Pj/2017 Tentang Cara Penelitian Bukti Pemenuhan Kewajiban Penyetoran Pajak Penghasilan Atas Penghasilan Dari Pengalihan Hak Atas Tanah Dan/Atau Bangunan, Dan Perjanjian Pengikatan Jual Beli Atas Tanah Dan/Atau Bangunan Beserta Perubahannya. Efektifitas pasal 1 Peraturan Direktur Jendral Pajak Nomor Per-18/Pj/2017 di Kantor Pajak Pratama Kota Malang telah sesuai akan tetapi untuk pasal 1 ayat 2 masih belum dan untuk pengikatan jual beli, dan mengenai cara pembuktian bahwa wajib pajak telah memenuhi kewajiban penyetoran adalah dengan cara validasi ataupun telah di telitiKata Kunci: kewajiban, penyetoran, pemenuhan, pajak, validasiThe Research Phase or what the author commonly refers to as SSP validation (Tax Payment Deposit) is the final stage in which a taxpayer has carried out his obligations in tax payments, and in the process of validation itself not all will be accepted by the local tax office because there will still be done a research both formal and material. For the mechanism of validation, it has been stipulated in Per-18 / Pj / 2017 Regarding the Method of Research of Evidence of Fulfillment of Obligation of Income Tax on Income from Transfer of Land and / or Building Rights, and Agreement on Binding of Sale and Purchase of Land and / or Buildings and Amendments. The effectiveness of article 1 of the Regulation of the Director General of Tax Number Per-18 / Pj / 2017 in the Pratama Tax Office Malang is appropriate but for article 1 paragraph 2 it is still not yet for the binding of buying and selling, and regarding the means of proving that the taxpayer has fulfilled the payment obligation is by validation or thoroughlyKeywords: oblilgation, deposit, fulfillment, tax, validatio
A Versatile and Efficient 0.1-to-11 Gb/s CML Transmitter in 40-nm CMOS
No full textWe present a wireline transmitter (TX) for re-configurable chip-to-chip links. The proposed design features a frequency-adaptive clock chain, a fast 16:1 clocked-CMOS multiplexer (C2MOS MUX) tree, and a full-rate synchronous current-mode logic (CML) clock driver. A prototype realized in 40-nm CMOS accomplishes a wide 0.1-to-11 Gb/s operation range (fmax/fmin = 110×). At 11 Gb/s, the prototype achieves 3.98 pJ/bit for a bit error rate (BER) < 10-12 with a 60.9-ps eye width.Accepted author manuscriptElectronic
A Differential Transmission Gate Design Flow for Minimum Energy Sub-10-pJ/Cycle ARM Cortex-M0 MCUs
No full textUltra-low voltage operation is key to achieving energy-efficient operation for microcontroller (MCU) systems. Variation resiliency, high speed operation, and short design time are the most important challenges for these systems. This paper overcomes these challenges in a new design strategy that enables standard cell design with differential transmission gate logic. The commercial toolchain is extended with in-house developed add-ons and makes use of two custom libraries with different device lengths to allow high speed vs. low leakage trade-offs. The design flow is used to prototype two highly efficient 32-bit ARM Cortex-M0 MCU systems in 40-nm CMOS. The core of the first prototype scales down to 190 mV and 0.8-MHz and reaches 16.07 pJ/cycle at 31.2-MHz and 440 mV. The second prototype benefits from the dual libraries and reduces core energy consumption by 50% at the same speed performance. Minimum energy operation is thus achieved at an even lower voltage (370 mV) with the M0 core consuming only 8.80 pJ/cycle at 13.7-MHz, breaking the sub-10-pJ/cycle barrier for a 6–35-MHz range.sponsorship: This paper was approved by Guest Editor Eugenio Cantatore. This work was supported by the IWT-Agency for Innovation by Science and Technology. (Corresponding author: Hans Reyserhove.) (IWT-Agency for Innovation by Science and Technology)status: Publishe
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