596 research outputs found
De-escalation of support with veno-arterial extracorporeal membrane oxygenation and Impella for cardiogenic shock: reply
No full textThe class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae.
No full textThe actin cytoskeleton is essential for polarized, bud-directed movement of cellular membranes in Saccharomyces cerevisiae and thus ensures accurate inheritance of organelles during cell division. Also, mitochondrial distribution and inheritance depend on the actin cytoskeleton, though the precise molecular mechanisms are unknown. Here, we establish the class V myosin motor protein, Myo2, as an important mediator of mitochondrial motility in budding yeast. We found that mutants with abnormal expression levels of Myo2 or its associated light chain, Mlc1, exhibit aberrant mitochondrial morphology and loss of mitochondrial DNA. Specific mutations in the globular tail of Myo2 lead to aggregation of mitochondria in the mother cell. Isolated mitochondria lacking functional Myo2 are severely impaired in their capacity to bind to actin. laments in vitro. Time-resolved. uorescence microscopy revealed a block of bud-directed anterograde mitochondrial movement in cargo binding-defective myo2 mutant cells. We conclude that Myo2 plays an important and direct role for mitochondrial motility and inheritance in budding yeast
Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p
No full textThe mitochondrial compartment of budding yeast (Saccharomyces cerevisiae) is a highly dynamic net-like structure of tubules that constantly undergo fusion and fission. The outer membrane protein Fis1p plays a crucial role in mitochondrial fission. Here we report on the temporal and spatial dynamics of this organelle in wildtype cells and in fis1Delta mutants. Mitochondria of fis1Delta mutants adapt their mitochondrial network to a change in carbon source. We find that the frequencies of apparent matrix separation and fusion events decrease in both wildtype cells and in mutants lacking Fis1Delta upon glucose repression. Matrix separation could be caused by matrix constriction and does not necessarily require fission of the inner or outer membrane. Double-labelling experiments demonstrated that some of these matrix separations in fis1 mutants are due to genuine tubule fissions, whereas others do not involve fission of the outer membrane. The rates of matrix separation in fis1Delta mutants almost approach those of the wildtype, demonstrating that, although apparently involved in outer membrane fission, Fis1p is not crucial for the separation of the mitochondrial matrix. In mutants lacking the GTPase Dnm1p no complete tubule fissions were recorded, although dnm1Delta mutants display matrix separations as well. The data suggest that different molecular machineries are responsible for the separation of the matrix and the fission of the outer membrane in budding yeast
The inner membrane protein Mdm33 controls mitochondrial morphology in yeast
No full textMitochondrial distribution and morphology depend on MDM33, a Saccharomyces cerevisiae gene encoding a novel protein of the mitochondrial inner membrane. Cells lacking Mdm33 contain ring- shaped, mostly interconnected mitochondria, which are able to form large hollow spheres. On the ultrastructural level, these aberrant organelles display extremely elongated stretches of outer and inner membranes enclosing a very narrow matrix space. Dilated parts of Deltamdm33 mitochondria contain well-developed cristae. Overexpression of Mdm33 leads to growth arrest, aggregation of mitochondria, and generation of aberrant inner membrane structures, including septa, inner membrane fragments, and loss of inner membrane cristae. The MDM33 gene is required for the formation of net-like mitochondria in mutants lacking components of the outer membrane fission machinery, and mitochondrial fusion is required for the formation of extended ring-like mitochondria in cells lacking the MDM33 gene. The Mdm33 protein assembles into an oligomeric complex in the inner membrane where it performs homotypic protein-protein interactions. Our results indicate that Mdm33 plays a distinct role in the mitochondrial inner membrane to control mitochondrial morphology. We propose that Mdm33 is involved in fission of the mitochondrial inner membran
Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast
No full textThe MDM31 and MDM32 genes are required for normal distribution and morphology of mitochondria in the yeast Saccharomyces cerevisiae. They encode two related proteins located in distinct protein complexes in the mitochondrial inner membrane. Cells lacking Mdm31 and Mdm32 harbor giant spherical mitochondria with highly aberrant internal structure. Mitochondrial DNA (mtDNA) is instable in the mutants, mtDNA nucleoids are disorganized, and their association with Mmm1-containing complexes in the outer membrane is abolished. Mutant mitochondria are largely immotile, resulting in a mitochondrial inheritance defect. Deletion of either one of the MDM31 and MDM32 genes is synthetically lethal with deletion of either one of the MMM1, MMM2, MDM10, and MDM12 genes, which encode outer membrane proteins involved in mitochondrial morphogenesis and mtDNA inheritance. We propose that Mdm31 and Md32 cooperate with Mm1 Mm2 Mdm10, and Mdm12 in maintenance of mitochondrial morphology and mtDNA
Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast
No full textThe MDM31 and MDM32 genes are required for normal distribution and morphology of mitochondria in the yeast Saccharomyces cerevisiae. They encode two related proteins located in distinct protein complexes in the mitochondrial inner membrane. Cells lacking Mdm31 and Mdm32 harbor giant spherical mitochondria with highly aberrant internal structure. Mitochondrial DNA (mtDNA) is instable in the mutants, mtDNA nucleoids are disorganized, and their association with Mmm1-containing complexes in the outer membrane is abolished. Mutant mitochondria are largely immotile, resulting in a mitochondrial inheritance defect. Deletion of either one of the MDM31 and MDM32 genes is synthetically lethal with deletion of either one of the MMM1, MMM2, MDM10, and MDM12 genes, which encode outer membrane proteins involved in mitochondrial morphogenesis and mtDNA inheritance. We propose that Mdm31 and Md32 cooperate with Mm1 Mm2 Mdm10, and Mdm12 in maintenance of mitochondrial morphology and mtDNA
Turism development posibilities in the Benedikt municipality
No full textMagistrsko delo obravnava turistični potencial občine Benedikt. V ospredje je avtorica postavila naravne in kulturne značilnosti, ki jih premore občina. Benedikt leži v osrčju Slovenskih goric. Nekoč je bil kraj povsem kmetijsko območje, v zadnjih dveh desetletjih pa so se v občini pričele razvijati tudi terciarne in kvartarne dejavnosti. Danes v občini prevladujejo polkmetije, saj občani poleg dela na kmetiji hodijo še v službo. V zadnjih nekaj letih se je center občine izjemno razvil, k temu je pripomogla hitra cesta, ki je odvzela promet, ki se je včasih vil mimo Benedikta. Prav tako je k selitvi mladih družin v Benedikt pripomogla gradnja stanovanjskih blokov in načrtna gradnja modernega stanovanjskega naselja v bližini. Razvijati se je začel turizem, občina je poskrbela za informativne table, razne poti povezujejo znamenitosti v celoto. Leta 2004 so končali z deli na vrtini termalne vode, ki je namenjena izgradnji termalnega kopališča v Benediktu.The Master\u27s thesis deals with the tourism potential of the Benedict Municipality. The author has highlighted the natural and cultural characteristics, which are present in the municipality. Benedikt is located in the heart of Slovenske gorice. Once the place was a fully agricultural area, but in the last two decades, the municipality began to develop in the tertiary and quaternary activities. Today, the municipality is dominated by part-time farming, as citizens in addition to working on the farm also go to work. In the last few years, the centre of the municipality has extremely developed. This happened because of the express road, which has taken away the traffic that used to go through Benedikt in the past. The construction of housing estate and systematic construction of modern residential area nearby have also contributed to young families moving to Benedikt. Tourism started to develop, the municipality took care of the information boards, and various paths are linking sights into a whole. In 2004, the works on the borehole of the thermal water, which is intended for the construction of thermal spa in Benedikt, were finished
khoehlein/Permutation-invariant-Postprocessing: Preliminary code release with DOI batch
No full textCode for paper "Postprocessing of Ensemble Weather Forecasts Using Permutation-invariant Neural Networks" by Kevin Höhlein, Benedikt Schulz, Rüdiger Westermann and Sebastian Lerch
Code "Postprocessing of Ensemble Weather Forecasts Using Permutation-invariant Neural Networks"
No full textCode for paper "Postprocessing of Ensemble Weather Forecasts Using Permutation-invariant Neural Networks" by Kevin Höhlein, Benedikt Schulz, Rüdiger Westermann and Sebastian Lerch
Innovation in traditional porcelain company G. Benedikt
No full textThis thesis recognizes the importance of innovation in an everchanging world, even for traditional companies. The aim of this thesis is to gain a comprehensive understanding of the factors that influence product innovation at G. Benedikt and identify trends and factors within the HoReCa sector that can help the company to remain competitive and profitable in the future. To achieve this author elaborated on the s the company’s approach towards innovation, evaluated the impact of being a family business on its innovativeness, explored the external factors, assessed how is G. Benedikt compared to its competitors in terms of innovation and market trends that may affect the development of new products. Additionally, the research seeks to uncover potential areas of focus and opportunities for further innovation to drive growth and maintain a competitive edge within the industry.Tato práce se věnuje důležitosti inovací v neustále se měnícím světě, a to i v tradiční společnosti. Cílem této práce je získat komplexní představu o faktorech, které ovlivňují inovaci výrobků ve společnosti G. Benedikt, a identifikovat trendy a faktory v odvětví HoReCa, které mohou společnosti pomoci udržet si konkurenceschopnost a ziskovost i v budoucnu. Za tímto účelem autorka zpracovala přístup společnosti k inovacím, zhodnotila vliv toho, že je rodinným podnikem, na její inovativnost, prozkoumala vnější faktory, posoudila, jak je společnost G. Benedikt srovnávána se svými konkurenty z hlediska inovací a tržních trendů, které mohou ovlivnit vývoj nových výrobků. Kromě toho se výzkum snažil odhalit potenciální oblasti, na které je třeba se zaměřit, a příležitosti pro další inovace, aby se podpořil růst a udržel konkurenční náskok v rámci odvětví
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