1,721,649 research outputs found
Supplemental Material, JCD-2018-0224.R1_Author's_bios - A Cross-Cultural Study of Calling and Life Satisfaction in the United States and South Korea
No full textSupplemental Material, JCD-2018-0224.R1_Author's_bios for A Cross-Cultural Study of Calling and Life Satisfaction in the United States and South Korea by Jina Ahn, Hye-Weon Kim and Jee-Yon Lee in Journal of Career Development</p
Establishment of a Simple and Rapid Method for Quantitative Determination of -Aminobutyric Acid Using<sup>1</sup>H NMR and Production of γ-Aminobutyric Acid in Cell Suspension Cultures of Coriandrum sativum L.
No full textThis study describes a simple and rapid method for quantitative determination of \gamma-aminobutyric acid (GABA) using ^1H NMR spectroscopy from whole cell extracts of plant suspension cultures. When 9 cell lines derived from 8 species of higher plants maintained in liquid Marashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) were subjected to ^1H NMR, a cell line of Coriandrum sativum L. exhibited the highest level of GABA. The level reached up to 16.9 mg/dry wt when cells were cultured in MS medium supplemented with 0.5 mg/L 2,4-D after 3 weeks of incubation. The method for quantitative determination of GABA using ^1H NMR established in this study could be applied to high-throughput screening of various plant resources for GABA production and the cell suspension culture system of C. sativum could be further developed for commercial production of GABA.open
A rapid, simple method for the determination of 6-benzyladenine by pyrolysis mass spectrometry
No full textPyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum. PyMS was used for the quantitative determination of 6-benzyladenine (BA) supplemented to agar-solidified culture media (ASM) in this study. When subjected to PyMS, pure BA generated prominent fingerprint peaks. The peaks at m/z 68 and 123 were chosen for the quantitative measurement of BA because of the highest signal among those generated from pure BA and because of one of the highest masses among those with a prominent signal, respectively. To establish a standard curve for BA concentration in ASM, the combined peak intensity at m/z 68 and 123 was plotted against BA concentration ranged from as low as 0. 44 μM after logarithmic transformation of both parameters. A linear regression line was yielded, which indicates BA concentration in ASM is directly proportional to the peak intensity, with R2 = 0. 9052, significant at the 99% level. These results suggest that PyMS enables the quantitative determination of growth regulators and other related compounds in plant materials in a rapid, simple, sensitive, accurate manner.open
Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower
No full textMature zygotic embryos of balloon flower (Platycodon grandiflorum) formed embryogenic calluses at a frequency of 43% when cultured on Murashige and Skoog medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Cell suspension cultures were established from embryogenic calluses using MS liquid medium with 4.52 μM 2,4-D. Following transfer to solid MS basal medium, cell suspension cultures gave rise to somatic embryos, which then developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity.open
Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis
No full textThis chapter describes culture conditions for high-frequency plant regeneration via somatic embryogenesis and cryopreservation from cell suspension cultures of Ranunculus kazusensis. Zygotic embryos form white nodular structures and pale-yellow calli at a frequency of 84.9% on half-strength Schenk and Hildebrandt (SH) medium supplemented with 0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4- D). However, the frequency of white nodular structure and off-white callus formation decreases to 25% with an increasing concentration of 2,4- D up to 10 mg/L cell suspension cultures are established from zygotic embryo-derived pale-yellow calli using half-strength SH medium supplemented with 0.1 mg/L 2,4- D. Upon plating onto half-strength SH basal medium, over 90% cell aggregates give rise to numerous somatic embryos and develop into plantlets. Regenerated plantlets are transplanted to pots filled with soil and grown to maturity at 90% survival rate in a growth chamber. Furthermore, we have developed the cryopreservation system using embryogenic cell suspension cultures of Ranunculus kazusensis. The re-growth rate of cryopreserved cells in 20% glycerol and 10% dimethylsulfoxide (DMSO) is 10% and 28.3%, respectively. These results show that DMSO is more effective cryoprotectant than glycerol in long-term preservation of embryogenic cell suspension cultures. The plant regeneration and cryopreservation system established in this study could be applied for mass propagation and ex situ conservation of this plant species.open
Plant Regeneration from Zygotic Embryos Cultures of Lilium Lancifolium Thunb. Via Bulblet Formation
No full textPlant regeneration system from zygotic embryos (2n=24) of Lilium lancifolium Thunb. via bulblet formation was estabished. Zygotic embryos of Lilium lancifolium formed bulblets and somatic embryos simultaneously when they cultured on MS medium supplemented with low concentration of 2,4-D. The highest frequency of bulblet and somatic embryo formation from zygotic embryos of Lilium lancifolium was 66.7% and 56.7%, respectively. The frequency of bulblet and somatic embryo formation was decreased when they cultured on MS medium over than 1 mg/L of 2,4-D. To regenerate whole plants, somatic embryos formed on zygotic embryos were transferred to MS basal medium. However somatic embryos did not fully converted into plantlets. Further incubation in the light, elongated somatic embryos formed numerous bulblets at the base of somatic embryos. Upon transfer to MS basal medium, bulblets were successfully converted into plantlets after further 4 weeks of culture in the light. After acclimatization, plantets from bulblets were transferred to soil and grown to normal plants in growth chamber (approximately , 16/8h photo period, ) The chromosome analysis revealed that plants regenerated from zygotic embryos showed 2n=24. These results indicate that chromosome stability of source tissue is maintained during plant regeneration via bulblet formationopen
Advances in plant metabolomics
No full textPlant metabolomics is a plant biology field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. For holistic approach, metabolomics frequently uses chemometrics or multivariate statistical analysis of metabolic profillings. In plant biology, metabolomics is useful to determine functions of genes often in combination with DNA microarrays by analyzing tagged mutants of the model plants Arabidopsis and rice. This review paper attempted to introduce basic concepts of metabolomics and practical uses of multivariate statistical analysis of metabolic profiling obtained by 1H NMR and Fourier transform infrared spectrometry.open
Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose
No full textRugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (1/2MS) supplemented with 2.26, 9.05, and 9.05 μM 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on 1/2MS without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.open
High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of coriander (Coriandrum sativum L.)
No full textHypocotyl segments and zygotic embryos of coriander formed embryogenic calli at frequencies of up to 75% when cultured on MS medium supplemented with 1 mgl-1 2,4-D. Calli were transferred to MS liquid medium with 1 mgl-1 2,4-D to initiate cell suspension cultures. Embryogenic cells became finely dispersible in the medium as the subculture proceeded. Cultures were transferred to a nitrogen compound enriched liquid MS medium containing 2% sucrose and 0.1 mgl-1 2,4-D, and cultured two weeks before plating on MS basal medium. Approximately 75% of cell aggregates (1 to two mm in diameter) underwent development into globular to cotyledonary somatic embryos after two weeks of plating. Most of the embryos were subsequently regenerated into plantlets. Regenerants were successfully transplanted to potting soil and grown to maturity in a phytotron.open
Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose
No full textImmature zygotic embryos of rose (Rosa hybrida L.; cv. Sumpath) did not form somatic embryos or embryogenic calluses when cultured on half-strength Murashige and Skoog's medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. However, the zygotic embryos produced somatic embryos without an intervening callus phase at a frequency of 27.3% on medium with 4.44 μM 6-benzyladenine (BA) alone. Immature zygotic embryos formed embryogenic calluses at a frequency of 25% on medium with a combination of 1.36 μM 2,4-D and 4.44 μM BA. Upon transfer to medium without growth regulators, embryogenic calluses produced numerous somatic embryos that subsequently developed into plantlets. Somatic embryos were induced directly from immature zygotic embryos, or indirectly via an intervening callus phase, by manipulating the exogenous growth regulators. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.open
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