5,390 research outputs found
Song wen xuan
No full textShang ce wei bei song bu fen ; xia ce wei nan song bu fen. mei pian wen zhang you zuo zhe jian jie ji ti jie he zhu sh
Supplemental Material - White Light Stimulation at Gamma Frequency to Modify the Aβ42 and tau Proteins in SH-SY5Y Cells
No full textSupplemental Material for White Light Stimulation at Gamma Frequency to Modify the Aβ42 and tau Proteins in SH-SY5Y Cells by Yang-Pei Chang, Ching-Fang Chien, Ling-Chun Huang, Chih-Pin Chuu, Hsi-Wen Chang, Tzyh-Chyuan Hour, and Yuan-Han Yang in American Journal of Alzheimer's Disease & Other Dementias®</p
Wellesly Sh. W. to Mr. James Meredith (2 October 1962)
Full text linkSigned by Wellesly Sh. W.https://egrove.olemiss.edu/mercorr_pro/1531/thumbnail.jp
Zhongguo xian dai wen xue de jiang jie The territory of modern Chinese literature
No full textBen shu bao kuo le bi zhe guo qu 10 nian fa biao yu xue shu qi kan de lun wen, zhu yao nei rong shi guan yu Zhongguo xian dai wen xue de zuo jia yan jiu, qi fan wei bao kuo ru qian suo shu de duo di qu wen xue xian xiang de lun xi, bi zhe li zu yu wen ben xi du, tong shi zhu yi dui shi dai yu jing, zhi shi fen zi ming yun de ti cha, shi tu cong yi ge bi jiao hong guan de shi ye zhong guan cha Zhongguo xian dai wen xue de fa zhan qing kuang. bi zhe dui Zhongguo xian dai wen xue jiang yu de yan jiu, shi yi zhong xin de wen xue shi guan de chang shi, zhe ben lun zhu zhong di lun wen shi dui guo qu yan jiu de zong jie, ye shi dui wei lai xin de yan jiu ke ti de qi shi he kai sh
Li yi zhong de mei shu Wu Hong Zhong guo gu dai mei shu shi wen bian = Art in its ritual context : essays on ancient Chinese art
No full textBen shu xuan yi le zhu ming mei shu shi jia wu hong jiao shou zi 1985 nian yi lai fa biao de 31 pian lun wen. zhe xie lun wen wei rao zhe " li yi mei shu " zhe yi ji ben gai nian tao lun le zhong guo shang gu he zhong gu mei shu zhong de duo xiang yi shu chuan tong he xing sh
Fig. 3 in Isolation, structure elucidation and neuroprotective effects of caffeoylquinic acid derivatives from the roots of Arctium lappa L.
No full textFig. 3. Effect of compounds(1–9,11–14) on cell viability. (A) Cytotoxic effect of different concentrations of H2O2 on SH-SY5Y cells. (B) 200 μM compounds(1–9,11–14) alone treated had no effect on cell viability. The date were represented as mean ± S.E.M of three independent experiments. ***p <0.001 compared with control.Published as part of Gao, Huan, Jiang, Xiao-Wen, Yang, Yue, Liu, Wen-Wu, Xu, Zi-Hua & Zhao, Qing-Chun, 2020, Isolation, structure elucidation and neuroprotective effects of caffeoylquinic acid derivatives from the roots of Arctium lappa L., pp. 1-7 in Phytochemistry (112432) 177 on page 5, DOI: 10.1016/j.phytochem.2020.112432, http://zenodo.org/record/829582
Continuous monitor of mixed venous oxygen saturation in pediatric cardiac surgical patient.
No full textSMA-SH: Modified styrene maleic acid copolymer for functionalization of lipid nanodiscs
No full textChallenges in purification and subsequent functionalization of membrane proteins often complicate their biochemical and biophysical characterization. Purification of membrane proteins generally involves replacing the lipids surrounding the protein with detergent molecules, which can affect protein structure and function. Recently, it was shown that styrene–maleic acid copolymers (SMA) can dissolve integral membrane proteins from biological membranes into nanosized discs. Within these nanoparticles, proteins are embedded in a patch of their native lipid bilayer that is stabilized in solution by the amphipathic polymer that wraps the disc like a bracelet. This approach for detergent-free purification of membrane proteins has the potential to greatly simplify purification but does not facilitate conjugation of functional compounds to the membrane proteins. Often, such functionalization involves laborious preparation of protein variants and optimization of labeling procedures to ensure only minimal perturbation of the protein. Here, we present a strategy that circumvents several of these complications through modifying SMA by grafting the polymer with cysteamine. The reaction results in SMA that has solvent-exposed sulfhydrils (SMA-SH) and allows tuning of the coverage with SH groups. Size exclusion chromatography, dynamic light scattering, and transmission electron microscopy demonstrate that SMA-SH dissolves lipid bilayer membranes into lipid nanodiscs, just like SMA. In addition, we demonstrate that, just like SMA, SMA-SH solubilizes proteoliposomes into protein-loaded nanodiscs. We covalently modify SMA-SH-lipid nanodiscs using thiol-reactive derivatives of Alexa Fluor 488 and biotin. Thus, SMA-SH promises to simultaneously tackle challenges in purification and functionalization of membrane proteins.BN/Marie-Eve Aubin-Tam LabBN/Andreas Engel La
Omega-3 fatty acid eicospentaenoic acid attenuates MPP+-induced neurodegeneration in fully differentiated human SH- SY5Y and primary mesencephalic cells
No full textEicosapentaenoic acid ( EPA), a neuroactive omega-3 fatty acid, has been demonstrated to exert neuroprotective effects in experimental models of Parkinson's disease ( PD), but the cellular mechanisms of protection are unknown. Here, we studied the effects of EPA in fully differentiated human SH-SY5Y cells and primary mesencephalic neurons treated with MPP+. In both in-vitro models of PD, EPA attenuated an MPP+-induced reduction in cell viability. EPA also prevented the presence of electron-dense cytoplasmic inclusions in SH-SY5Y cells. Then, possible mechanisms of the neuroprotection were studied. In primary neurons, EPA attenuated an MPP+-induced increase in Tyrosine-related kinase B (TrkB) receptors. In SH-SY5Y cells, EPA down-regulated reactive oxygen species and nitric oxide. This antioxidant effect of EPA may have been mediated by its inhibition of neuronal NADPH oxidase and cyclo-oxygenase-2 ( COX-2), as MPP+ increased the expression of these enzymes. Furthermore, EPA prevented an increase in cytosolic phospholipase A2 ( cPLA2), an enzyme linked with COX-2 in the potentially pro-inflammatory arachidonic acid cascade. Lastly, EPA attenuated an increase in the bax:bcl-2 ratio, and cytochrome c release. However, EPA did not prevent mitochondrial enlargement or a decrease in mitochondrial membrane potential. This study demonstrated cellular mechanisms by which EPA provided neuroprotective effects in experimental P
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