6,965 research outputs found
Correction to Virus Bioresistor (VBR) for the Detection of the Bladder Cancer Marker DJ-1 in Urine at 10 pM in One Minute
No full textThe author list contained an error: Gregory A. Weiss was not identified as a corresponding author. Both of the corresponding authors, Gregory A. Weiss and Reginald M. Penner, are indicated in this Addition and Correction
Pope Gregory X and the crusades
No full textPhDThis study examines the crusading movement during the reign of Pope Gregory X in the latter part of the thirteenth century, before the Latin presence in the Levant came to an end. It seeks to demonstrate the important position of this little-known pope, who formed the bridge between what can now be seen as two separate eras in the crusading period, namely, the era of the traditional passagium generale, and the ‘new’ era of the passagium particulare. To do this, it will study Western and Muslim sources to understand the condition of the Holy Land during Gregory’s pontificate to see the effect it had on the manner in which he organised his crusade, using both traditional and ‘new’ methods. By drawing on sources from crusading in Iberia, it will show that Gregory approached the crusade flexibly, and was not, as commonly described by historians, wholly obsessed with the Holy Land. It also seeks to dispel one of the more popular myths surrounding Gregory, which is that he wanted to change the government of the kingdom of Jerusalem by putting Charles of Anjou in charge there. A study of the Angevin chancery records – little used by crusade historians – will demonstrate that it was not Gregory’s idea, but rather Charles’ own. Finally, using Gregory’s papal registers and chronicle evidence, this study will attempt to imagine the crusade that would have occurred had Gregory not died prematurely. This includes a discussion of the unprecedented scope of its recruitment as evidence of Gregory’s exceptional ability as a crusade organiser, as well as the evidence and reasons for a dramatic change in direction away from Egypt.Overseas research Students Awards Scheme provided by the British Council
The Rhetoric of Landscape in Gregory of Nyssa’s Homilies on the Song of Songs
No full textThis is the author accepted manuscript. The final version is available from Brill via the ISBN in this recordAnalytical and Supporting Studies. Proceedings of the 13th International Colloquium on Gregory of Nyssa (Rome, 17-20 September 2014)Series:
Vigiliae Christianae, Supplements, Volume: 150In this paper I want to take you on a walk through a garden. It is, to be sure, an imaginary garden; nevertheless, it bears a significance which extends beyond itself. Some of this significance concerns words and texts: for as we shall see, the garden is, amongst other things, a ‘garden of rhetoric’. The garden in question appears in the Gregory of Nyssa’s Homilies on the Song of Songs.[...
An Evening with Richard Claxton “Dick” Gregory, Civil Rights Activist, Nutritionist, Comedian, and Author
No full textGregory, Richard Claxton “Dick” (Born, October 12, 1932, St. Louis, Mo.), African American comedian and civil rights activist whose social satire changed the way white Americans perceived African American comedians since he first performed in public. Gregory’s autobiography, Nigger, was published in 1963 prior to The assassination of President Kennedy, and became the number one best-selling book in America. Over the decades it has sold in excess of seven million copies. His choice for the title was explained in the forward, where Dick Gregory wrote a note to his mother. “Whenever you hear the word ‘Nigger’,” he said, “you’ll know their advertising my book.” In 1984 he founded Health Enterprises, Inc., a company that distributed weight loss products. In 1987 Gregory introduced the Slim-Safe Bahamian Diet, a powdered diet mix, which was immensely profitable. Economic losses caused in part by conflicts with his business partners led to his eviction from his home in 1992. Gregory remained active, however, and in 1996 returned to the stage in his critically acclaimed one-man show, Dick Gregory Live! The reviews of Gregory’s show compared him to the greatest stand-ups in the history of Broadway
“Judge Me Gently”: Reflections on the Religious Life of John Milton Gregory, 1822–1898
No full text John Milton Gregory is familiar to many Christian educators through his 19th-century publication, The Seven Laws of Teaching. For most readers of this important book, little is known about the author himself. This article explores the religious life and theological foundations of John Milton Gregory, who was both author of The Seven Laws of Teaching and founding president of the University of Illinois. Utilizing his spiritual diaries preserved in his daughter's biography of her father and archival sources from the University of Illinois, this essay offers a theological and spiritual understanding of this important historical figure. </jats:p
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Advancing DNA Sequencing Technologies, Antibody Therapeutics, and Viral Diagnostics
No full textDNA polymerase catalyzes the correct replication and repair of DNA, an essential step in the life cycle of all organisms. The enzyme incorporates incoming deoxynucleotide triphosphates (dNTPs) into a nascent DNA strand that is complementary to a single-stranded DNA (ssDNA) template. This capability makes DNA polymerases workhorses for molecular biology and biotechnology. Single-molecule studies can identify the dynamics of DNA polymerase structural conformations, which are otherwise lost through averaging in ensemble populations. Therefore, the Weiss and Collins labs (UCI) collaboratively developed a single-walled carbon nanotube field-effect transistor (nanocircuit) to translate enzyme motions into electronic signals. These nanocircuits may illuminate hidden conformational transitions of DNA polymerase activity and reveal new mechanisms and dynamics during dNTP incorporation. The goal of this work is to apply this approach to understand the conformational dynamics of the thermostable DNA polymerase from Thermus aquaticus (Taq) and processive DNA polymerase from the Bacillus subtilis bacteriophage phi29 (Φ29). The work here resolves subtle dynamics and transient intermediate states of DNA polymerase that modulate catalytic speed and molecular recognition during DNA synthesis. Additional efforts are focused on evaluating the performance of nanocircuits for direct electrical monitoring of polymerase in future DNA sequencing. Advances in bioconjugation, the ability to link biomolecules to each other, small molecules, surfaces, and more, can spur the development of advanced materials and therapeutics. In work reported here, pyrocinchonimide (Pci) undergoes a surprising transformation with biomolecules. The reaction targets amines and involves an imide transfer, which has not been previously reported for bioconjugation purposes. The Pci motif can reduce combinatorial diversity when many available reactive amines are available, such as in the formation of antibody-drug conjugates. The reaction offers a thermodynamically controlled route to single or multiple modifications of proteins for a wide range of applications. In response to the ongoing Coronavirus disease (COVID-19) pandemic, my laboratory members and I investigated the antibody response of patients infected by SARS-CoV-2 for predicting disease trajectories. Using methods of enzyme-linked immunosorbent assay (ELISA) and coronavirus antigen microarray (COVAM) analysis, antibody epitopes were mapped in the plasma of COVID-19 patients (n = 186) experiencing a wide range of disease states. This work identified antibodies to a 21-residue epitope from nucleocapsid (termed Ep9) associated with severe disease, including admission to the intensive care unit (ICU), requirement for ventilators, or death. By combining a disease risk factor score with a test for anti-Ep9 antibodies, severe COVID-19 outcomes could be predicted with 13.4 likelihood ratio (96.7% specificity). The results lay the groundwork for a new type of COVID-19 prognostic that could guide more effective therapeutic intervention
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Detection of DNA Polymerase I (Klenow Fragment) Activity Using Single-Molecule Electronic Nanocircuits and Toward the In Vitro Evolution of High-Affinity Binding Partners for Membrane Proteins From Phage-Displayed Affinity Reagent Libraries
No full textDNA polymerases are central biocatalysts in the life cycle of all organisms. Structural transitions of this enzyme during the incorporation of deoxynucleoside triphosphate (dNTP) substrates govern the accurate replication of DNA. Biophysical tools have emerged as useful techniques for understanding these structural transitions, and are continually being improved. Electronic-based techniques, in particular, provide many advantages over other biophysical techniques, including long duration monitoring and enhanced time resolution. For example, recently developed single-walled carbon nanotube field-effect transistors that measure DNA polymerase I Klenow Fragment (KF) activity serve as a valuable method for label-free detection of biocatalysis. In my Ph.D. work, I have sought to develop this technique further to better understand enzyme activity and take advantage of our observations for biotechnological applications. Incorporation of dNTP analogs with KF-functionalized nanocircuits challenged the molecular recognition of these substrates by KF. For some dNTP analogs, an alternative motion of KF during dNTP incorporation was observed and may be valuable in the discrimination of DNA bases in a template. The nanocircuits also accurately identified DNA template lengths when such templates were composed of highly repetitive sequences. Accumulation of several data sets further enabled precise measurement of DNA template lengths. Initial experiments of dNTP incorporation with a KF nanocircuit measured at high bandwidths demonstrated that the accurate measurement of DNA template lengths required the lower time resolution. The higher bandwidth experiments revealed previously unobserved KF motions that lasted for short durations, making this technique unsuitable for counting DNA bases. However, improvement to KF nanocircuits measured at high bandwidth can provide a powerful tool for understanding transient KF dynamics.To better understand and improve KF nanocircuits, a variety of KF variants were designed and generated. Many of these variants exhibited polymerase activity at the single-molecule level, and are in various stages of progression for their measurement with polymerase-functionalized nanocircuits. The experiments necessary to determine their viability in improving and understanding the electronic signals generated by KF were outlined for future Weiss lab members. Membrane proteins are a class of biomolecules that also benefit from the improved understanding of their molecular mechanisms. In vitro evolution of high affinity binding partners with stable, rigid phage-displayed protein libraries were attempted for the Helicobacter pylori inner membrane protein Ure-I, but were unsuccessful due to Ure-I’s rapid degradation. An alternative method for in vitro evolution for high affinity binding partners to the Shigella dysenteriae outer membrane protein ShuA was optimized to maintain ShuA in a native membrane environment
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Homeric Echoes in Posidippus
No full textLabored in Papyrus Leaves: Perspectives on an Epigram Collection Attributed to Posidippus (P.Mil.Vogl. VIII 309) is a Center for Hellenic Studies publication, specifically Hellenic Studies Series 2. Since Gregory Nagy is both article author and Director of the Center for Hellenic Studies, no special permission needed for online submission.The ClassicsAuthor's Origina
Spiritual Formation and the Body–Soul Relationship in Gregory of Nyssa
No full textThis is the author accepted manuscript. The final version is available from OUP via the link in this recor
SIDE 1: Dick Gregory
No full textThis audio recording features an episode of Martin Luther King, Jr./SCLC Speaks. In this episode, as stated by the narrator, the "wit and wisdom" of comedian and civil rights leader Dick Gregory are featured. Gregory speaks about racism in America and the struggles of the civil rights movement as well as commentary on the healthcare system. Gregory includes moments of levity in his remarks, such as where he speaks about Michael Jackson's adoption of a chimpanzee as a pet.The Atlanta University Center Robert W. Woodruff Library acknowledges the generous support of the Joseph & Evelyn Lowery Institute for Justice and Human Rights, the Joseph Echols Lowery Irrevocable Trust, and other donors in supporting the processing and digitization of Morehouse College's Joseph Echols and Evelyn Gibson Lowery Collection
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