7 research outputs found
RENOPROTECTIVE POTENTIAL OF FLAVONOIDS-RICH AGAINST DOXORUBICIN-INDUCED IN ANIMAL MODELS: A REVIEW
No full textCancer significantly impacts human health, affecting one in five people during their lifetime. While chemotherapeutic agents like doxorubicin are crucial in treating various cancers, they are also associated with severe side effects, including nephrotoxicity. This review examines the renoprotective potential of flavonoids against doxorubicin-induced renal damage in animal models. Doxorubicin works by intercalating Deoxyribo Nucleic Acid (DNA) and making Reactive Oxygen Species (ROS), which cause apoptosis and the death of cells. A thorough literature analysis was done to collect relevant papers on the impact of flavonoid-rich therapies as renoprotective agents against doxorubicin-induced nephrotoxicity. Databases such as Google Scholar, Scopus, PubMed, Springer, Wiley Online Library, and ScienceDirect were searched using keywords including "flavonoids, doxorubicin, renoprotective, nephrotoxicity, and animal model," focusing on publications from 2014 to 2024. Flavonoids are diverse polyphenolic compounds in many plants with significant pharmacological properties such as antioxidant, anti-inflammatory, and anticancer effects. This review highlights the renoprotective potential of flavonoids like quercetin, rutin, kaempferol, morin, luteolin, apigenin, hesperidin, naringenin, diosmin, and anthocyanins. These compounds reduce renal toxicity through mechanisms that decrease ROS, lipid peroxidation, mitochondrial permeability, and apoptosis
Moringa oleifera Leaf Extract and (Amorphophallus muelleri Blume.) Glucomannan Effects on Obese White Rat Feed Intake and Body Weight
Get PDFObesity significantly raises the rates of illness and death in humans due to its association with several health conditions such as cardiovascular disease, hypertension, hypercholesterolemia, type 2 diabetes mellitus, osteoporosis, depression, breast cancer, and colon cancer. This study aims to determine the effect of Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) on feed intake and body weight of obese white rats. This study used an experimental method with a pretest-posttest-only control grub design using 24 white male rats of the Wistar strain aged 8 weeks which were divided into 2 control groups, namely positive(K1) and negative(K2) and six treatment groups with extract doses Moringa oleifera leaves and glucomannan (Amorphophallus muelleri Blume.) 100mg/kgBW:100mg/kgBW(K3), 80mg/kgBW:120mg/kgBW(K4), 120mg/kgBW: 80mg/kgBW(K5), 50mg/kgBW:50mg/kgBW(K6), 40mg/kgBW:60mg/kgBW(K7), and 60mg/kgBW:40mg/kgBW(K8). Data analysis on body weight and feed intake used the SPSS ANOVA test and paired T-test. The results of the paired T-test (p<0.05) showed that Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) significantly reduced body weight and feed intake. In conclusion, administration of Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) in group K4 (80 mg/kgBW Moringa oleifera leaf extract: 120 mg/kgBW glucomannan Amorphophallus muelleri Blume) can reduce feed intake (4.29 g) and body weight (36.67 g) effectively
Combination effectiveness of glucomannan and moringa oleifera leaf extract on lipid profil of hypercholesterolemia rats
No full textcholesterol levels in the blood. This study aims to determine the effectiveness of the combination of glucomannan (Amorphophallus muelleri Blume) and Moringa oleifera leaf extract on the lipid profile of hypercholesterolemic Wistar rats. This research design used True experimental with a pretest-posttest control group design. The animal used in this study was 24 male Wistar rats (Rattus norvegicus) aged 8 weeks divided into 2 control groups and 6 treatment groups of glucomannan and Moringa oleifera leaf extract. The results of this study showed that there were significant changes in TC, TG, LDL-C, and HDL-C levels (p<0.05). It can be concluded that the combination of glucomannan (Amorphophallus muelleri Blume) and Moringa oleifera leaf extract can change the lipid profile effectively in the P5 (80mg/kgBW GAmB: 120mg/kgBW MoEL) and P4 (120mg/kgBW GAmB:80mg/kgBW MoEL ) as the most influential group in reducing TG, TC, LDL-C levels and increasing HDL-C levels
MOLECULAR DOCKING AND PHARMACOKINETIC EVALUATION OF NANOHERBAL SENDUDUK BULU (MICONIA CRENATA (VAHL.) MICHELANG.) COMPOUNDS AS AKT1 INHIBITORS
No full textObjective: This study seeks to investigate the potential of 36 nanoherbal compounds extracted from senduduk bulu (Miconia crenata (Vahl) Michelang.) as inhibitors of v-akt murine thymoma viral oncogene homolog 1 (AKT1) using molecular docking techniques, pharmacokinetic analysis, safety evaluation, and bioactivity assessment.
Methods: Senduduk bulu leaves were nanoparticle-processed and analyzed via Gas Chromatography-Mass Spectrometry (GC-MS). Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) profiles and biological activities were predicted, and molecular docking assessed compound interactions with AKT1 using borussertib as a reference.
Results: Findings indicate that 20 out of 36 compounds meet the criteria as drug candidates, demonstrating favorable interactions with the AKT1 protein, although their affinity did not surpass that of the positive control, borussertib. Several compounds exhibited high oral bioavailability, showed no interaction with the liver enzyme Cytochrome P450 2D6 (CYP2D6), and did not inhibit the Organic cation transporter 2 (OCT2) protein in the kidneys. In terms of toxicity, these compounds displayed a range of effects, from non-hazardous to hazardous, with some potentially posing risks of hepatotoxicity, carcinogenicity, and mutagenicity.
Conclusion: This research highlights the potential of nanoherbal senduduk bulu in cancer therapy development; however, further validation through in vitro and in vivo studies is necessary to comprehensively ensure their efficacy and safety
Formulasi Mi Instan Substitusi Tepung Krokot (Portulaca oleracea L.) Dan Glukomanan Sebagai Pangan Fungsional Dengan Variasi Suhu Pengeringan
Get PDFSalah satu upaya yang dapat dilakukan untuk mengurangi ketergantungan penggunaan tepung terigu pada mi instan yaitu dengan melakukan subtitusi komposisi bahan baku mi instan dengan melakukan riset terkait formulasi mi instan subtitusi tepung krokot (Portulaca oleracea L.) dan glukomanan sebagai pangan fungsional dengan variasi suhu pengeringan. Pembuatan mi instan sebagai pangan fungsional juga memerlukan pengeringan yang tepat sebab pengeringan memiliki dampak terhadap tinggi rendahnya penurunan kandungan gizi pada bahan pangan hingga dapat mempengaruhi mutu pangan
The Substitution of Purslane Weed (Portulaca oleracea L.) Flour and Glucomannan Powder on Organoleptic and Proximate Test of Instant Noodles
Get PDFInstant noodles are a food product that the public highly favors because of their diverse taste, easy presentation, and economical price. Instant noodles cannot be considered whole food because they do not meet the needs of balanced nutrition for the body. Purslane flour (Portulaca oleracea L.) contains macronutrients and micronutrients in the form of carbohydrates, protein, dopamine, potassium salts, tannins, saponins, vitamin A (β-carotene), Vitamin B, Vitamin C, nicotinic acid and natural antioxidants related to fatty acids omega 3 by 44.29%. This study contributed to compare the effectiveness of the substitution of Purslane flour and glucomannan powder on organoleptic test instant noodles. This study was an experiment with a completely randomized design of two-factorial with six treatments including the formulation of purslane flour 25%, 30%, and 35%, while glucomannan formulation of 5%, 10%, 15% with two temperature treatments, 50 °C, and 60 °C. We conducted an organoleptic test with statistical analysis t-test and descriptive. The substitution of purslane flour and glucomannan in instant noodles gave the best organoleptic characteristics in the K2S2 treatment (the formulation of 60% wheat flour, 30% purslane flour, and 10% glucomannan at a drying temperature of 60 °C) with proximate analysis content of 12.26% moisture, 5.53% ash, 5.13% fat, 13.91% protein, and 63.14% carbohydrates
Molecular Docking and in vivo Toxicity Evaluations of Jopan Nanoherbal (Clibadium surinamense L.) Leaves in a Zebrafish Model
Get PDFNanotechnological advancements have significantly increased the effectiveness of herbal remedies, particularly Jopan (Clibadium surinamense L.) leaves, which are recognised for their anti-inflammatory, antimicrobial, and antioxidant characteristics. This study aims to assess the toxicological potential of nanoherbal C. surinamense leaves via computational (molecular docking) and in vivo toxicity evaluations in a zebrafish model. ProTox-III was employed for the toxicity classifications; molecular docking simulations, using the CYP450 enzyme (PDB ID: 4R20); and in vivo zebrafish toxicity testing. Zebrafish were exposed to nanoherbal C. surinamense leaves at concentrations of 0, 12.5, 25, 50, 100, 200, 400, and 800 mg/L for 96 hours following OECD Guideline 203. The acute toxicity was evaluated by determining the LC50 value, while the toxic effects on the brain, liver, and intestinal tissues were assessed via histopathological analysis. An LC50 value of 516.87 mg/L was obtained, indicating low toxicity, while concentrations ≥200 mg/L caused dose-dependent toxic effects, including Purkinje cell degeneration, hepatocellular necrosis, and villus fragmentation. Molecular docking simulations revealed 2-undecanone 2,4-dinitrophenylhydrazone as the most active compound, exhibiting the strongest binding affinity (-7.4 kcal/mol) for CYP450. In conclusion, while nanoherbal C. surinamense leaves show therapeutic potential, their toxicity at higher concentrations necessitates further investigation to establish safe dosages; further, their long-term effects and pharmacokinetic properties should be explored to ensure their safety for medical applications
