241,414 research outputs found

    Walter E. Waters to James C. Furman

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    A two page letter and envelope from from Walter E. Waters to James C. Furma

    Developing elite Neurospora crassa strains for cellulosic ethanol production using fungal breeding

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    The demand for renewable and sustainable energy has generated considerable interest in the conversion of cellulosic biomass into liquid fuels such as ethanol using a filamentous fungus. While attempts have been made to study cellulose metabolism through the use of knockout mutants, there have been no systematic effort to characterize natural variation for cellulose metabolism in ecotypes adapted to different habitats. Here, we characterized natural variation in saccharification of cellulose and fermentation in 73 ecotypes and 89 laboratory strains of the model fungus Neurospora crassa. We observed significant variation in both traits among natural and laboratory generated populations, with some elite strains performing better than the reference strain. In the F1 population N345, 15% of the population outperformed both parents with the top performing strain having 10% improvement in ethanol production. These results suggest that natural alleles can be exploited through fungal breeding for developing elite industrial strains for bioethanol production.Peer reviewe

    Surveillance of adenoviruses and noroviruses in European recreational waters

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    Exposure to human pathogenic viruses in recreational waters has been shown to cause disease outbreaks. In the context of Article 14 of the revised European Bathing Waters Directive 2006/7/EC (rBWD, CEU, 2006) a Europe-wide surveillance study was carried out to determine the frequency of occurrence of two human enteric viruses in recreational waters. Adenoviruses were selected based on their near-universal shedding and environmental survival, and noroviruses (NoV) selected as being the most prevalent gastroenteritis agent worldwide. Concentration of marine and freshwater samples was done by adsorption/elution followed by molecular detection by (RT)-PCR. Out of 1410 samples, 553 (39.2%) were positive for one or more of the target viruses. Adenoviruses, detected in 36.4% of samples, were more prevalent than noroviruses (9.4%), with 3.5% GI and 6.2% GII, some samples being positive for both GI and GII. Of 513 human adenovirus-positive samples, 63 (12.3%) were also norovirus-positive, whereas 69 (7.7%) norovirus-positive samples were adenovirus-negative. More freshwater samples than marine water samples were virus-positive. Out of a small selection of samples tested for adenovirus infectivity, approximately one-quarter were positive. Sixty percent of 132 nested-PCR adenovirus-positive samples analysed by quantitative PCR gave a mean value of over 3000 genome copies per L of water. The simultaneous detection of infectious adenovirus and of adenovirus and NoV by (RT)PCR suggests that the presence of infectious viruses in recreational waters may constitute a public health risk upon exposure. These studies support the case for considering adenoviruses as an indicator of bathing water quality

    Letter Written by Merrill C. Waters to the Bryant College Service Club Dated December 20, 1942

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    [Transcription begins] WAR DEPARTMENT Services of Supply Headquarters Columbus Quartermaster Depot Columbus, Ohio December 20/42 Bryant Service Club Bryant College Providence, R.I. Many thanks for the kind remembrance which arrived safely this past week, after chasing me around the country. I am stationed at the Columbus Quartermaster Depot at present and have met several Bryant boys that are also stationed out here. My present address is listed above. Sincerely yours, Merrill C. Waters ‘36 2nd Lt. Q.M.C. [Transcription ends

    Hydrogen and oxygen isotope ratios of thermal waters of Okayama Prefecture, Japan

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    Hydrogen and oxygen isotope rations of thermal waters from 46 spas in Okayama Prefecture range from -62.6 to -29.2% in δD and from -10.0 to -4.4% in δ18O, respectively. The isotope rations indicate that all but one of the thermal water in Okayama prefecture are meteoric in origin. The Ofuku thermal water is the only exception, which is probably a mixture of seawater and meteoric water with the ratio of about 1. Sulfur isotope rations of dissolvel sulfate in the thermal waters range from -6.2 ti 59.3% in δ34S. The high δ34S values observed in some thermal waters may be due to bacterial reduction of sulfate

    Front parlor of the Meeker Memorial Museum

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    Three women wearing costumes gathered around the piano in front parlor of the Meeker Memorial Museum. Hanging above the piano is a hair weaving. ; Verso In pencil, 'Models in historic costumes gather around square grand piano in front parlor of the Meeker Home Museum, 1324 9th Avenue, Greeley, Colo. Cherrywood secretary on the left belonged to N. C. Meeker. Wallpaper & Brussels carpet are reproductions. Photo taken ca. January, 1961, by Bob Waters.' Stamped in purple ink is a bear holding a camera and the words, 'Bob Waters PHOTOGRAPHER COLORADO STATE COLLEGE GREELEY.

    Waters, C.

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    Average seasonal changes in chlorophyll a in Icelandic waters

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    The standard algorithms used to derive sea surface chlorophyll a concentration from remotely sensed ocean colour data are based almost entirely on the measurements of surface water samples collected in open sea (case 1) waters which cover ~60% of the worlds oceans, where strong correlations between reflectance and chlorophyll concentration have been found. However, satellite chlorophyll data for waters outside the defined case 1 areas, but derived using standard calibrations, are frequently used without reference to local in situ measurements and despite well-known factors likely to lead to inaccuracy. In Icelandic waters, multiannual averages of 8-d composites of SeaWiFS chlorophyll concentration accounted for just 20% of the variance in a multiannual dataset of in situ chlorophyll a measurements. Nevertheless, applying penalized regression spline methodology to model the spatial and temporal patterns of in situ measurements, using satellite chlorophyll as one of the predictor variables, improved the correlation considerably. Day number, representing seasonal variation, accounted for substantial deviation between SeaWiFS and in situ estimates of surface chlorophyll. The final model, using bottom depth and bearing to the sampling location as well as the two variables mentioned above, explained 49% of the variance in the fitting dataset

    Estimating specific inherent optical properties of tropical coastal waters using bio-optical model inversion and in situ measurements: case of the Berau estuary, East Kalimantan, Indonesia

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    Specific inherent optical properties (SIOP) of the Berau coastal waters were derived from in situ measurements and inversion of an ocean color model. Field measurements of water-leaving reflectance, total suspended matter (TSM), and chlorophyll a (Chl a) concentrations were carried out during the 2007 dry season. The highest values for SIOP were found in the turbid waters, decreasing in value when moving toward offshore waters. The specific backscattering coefficient of TSM varied by an order of magnitude and ranged from 0.003 m2 g-1, for clear open ocean waters, to 0.020 m2 g-1, for turbid waters. On the other hand, the specific absorption coefficient of Chl a was relatively constant over the whole study area and ranged from 0.022 m2 mg-1, for the turbid shallow estuary waters, to 0.027 m2 mg-1, for deeper shelf edge ocean waters. The spectral slope of colored dissolved organic matter light absorption was also derived with values ranging from 0.015 to 0.011 nm-1. These original derived values of SIOP in the Berau estuary form a corner stone for future estimation of TSM and Chl a concentration from remote sensing data in tropical equatorial water

    Stabilizing periodic orbits above the elliptic plane in the solar sail 3-body problem

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    We consider periodic orbits high above the ecliptic plane in the Elliptic Restricted Three-Body Problem where the third massless body is a solar sail. Periodic orbits above the ecliptic are of practical interest as they are ideally positioned for the year-round constant imaging of, and communication with, the poles. Initially we identify an unstable periodic orbit by using a numerical continuation from a known periodic orbit above the ecliptic in the circular case with the eccentricity as the varying parameter. This orbit is then used to construct a reference trajectory for the sail to track. In addition we illustrate an alternative method for constructing a periodic reference trajectory based on a time-delayed feedback mechanism. The reference trajectories are then tracked using a linear feedback regulator (LQR) where the control actuation is delivered by varying the solar sails orientation. Using this method it is shown that a 'near term' solar sail is capable of performing stable periodic motions high above the ecliptic
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