1,028 research outputs found
Drought Resistance Evaluation of <i>Camellia oleifera</i> var. “Xianglin 210” Grafted onto Different Rootstocks
No full textAs a key economic tree in southern China, Camellia oleifera faces severe yield losses under drought. Grafting onto drought-tolerant rootstocks offers a potential mitigation strategy. To elucidate the impact of rootstocks on the drought resistance of the superior Camellia oleifera Abel. cultivar “Xianglin 210”, grafted seedlings with five scion–rootstock combinations, were subjected to gradient drought stress. Key physiological and biochemical indices related to photosynthesis, antioxidant enzymes, and osmotic adjustment were measured. Drought resistance was comprehensively evaluated using membership function analysis, and the expression of stress-responsive genes was quantified via quantitative real-time PCR (qRT-PCR). The results demonstrated that under drought stress, (1) stomatal conductance (Gs) decreased by 31.2–48.7%, while instantaneous water use efficiency (WUE) increased by 18.5–35.4%; (2) proline (Pro) and soluble sugars (SS) accumulated significantly, with increases of 2.3–4.1-fold and 1.8–3.2-fold, respectively; (3) activities of antioxidant enzymes were enhanced by 56–127%, mitigating oxidative damage; (4) membership function analysis ranked drought resistance as follows: Xianglin 27 (0.812) > Guangxi Superior Germplasm (0.698) > C. yuhsienensis (0.654) > Hunan Superior Germplasm (0.591) > Xianglin 1 (0.523); (5) qRT-PCR revealed significant upregulation of ABA signaling pathway genes (CoPYL6, CoPP2C75/51/24/26, CoSnRK2.8, and CoABI5) and transcription factors (CoLHY and CoWRKY70), indicating activation of drought-responsive regulatory networks. These findings provide a theoretical foundation for selecting drought-tolerant rootstocks and optimizing cultivation practices in Camellia oleifera, and provide practical criteria for selecting drought-tolerant rootstocks, facilitating sustainable Camellia oleifera cultivation in water-limited regions
A polysaccharide from Andrographis paniculata induces mitochondrial-mediated apoptosis in human hepatoma cell line (HepG2)
No full textIn the present study, we investigated the effects and action mechanisms of a purified polysaccharide (APWP) from Andrographis paniculata, on human hepatocellular carcinoma (HCC) HepG2 cells. The results showed that APWP was able to suppress the proliferation of HepG2 cells via inducing apoptosis. Western blot analysis revealed that dose-dependent increase in proapoptotic Bax protein and no change in antiapoptotic Bcl-2 protein in APWP-treated cells. Furthermore, exposure of tumor cells to APWP resulted in a loss of mitochondrial membrane potential (MMP) and the release of cytochrome c from the mitochondria to the cytosol. Besides, caspase-9 and caspase-3 were activated while caspase-8 was not affected in HepG2 cells followed by APWP treatment. All these results point clearly to the involvement of mitochondria-mediated signaling pathway in APWP-induced apoptosis and strongly suggest that APWP seems to be safe and effective in the prevention and treatment of HCC
A polysaccharide from Huaier induced apoptosis in MCF-7 breast cancer cells via down-regulation of MTDH protein
No full textIn this study, one homogeneous polysaccharide (SP1), with a molecular weight of 56 kDa, was isolated from the Huaier fruiting bodies. It had a backbone consisting of 1, 4-linked-?-D-Galp and 1, 3, 6-linked-?-D-Galp residues, which was terminated with 1-linked-?-D-Glcp and 1-linked-?-L-Araf terminal at O-3 position of 1, 3, 6-linked-?-D-Galp unit along the main chain in the ratio of 1.1: 2.0: 1.1: 1.1. MTT assay showed that shMTDH or SP1 (100, 200 and 400 ?g/ml) was able to suppress the proliferation of MCF-7 cells, due to a significant increase in the number of apoptotic cells as determined by flow cytometric analysis. Furthermore, Western blot analysis revealed that SP1 or shMTDH treatment led to a rise of ratio between proapoptotic Bax and antiapoptotic Bcl-2 protein in MCF-7 cells. In addition, carcinogene MTDH protein expression in MCF-7 cells received SP1 (100, 200 and 400 ?g/ml) or shMTDH treatment was also repressed after 48 h incubation. Taken together, these findings indicated that SP1 has anticancer potential in the treatment of human breast cance
A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth, angiogenesis, and metastasis in nude mice
No full textMushroom Huaier has become a focus of interest in the treatment of hepatocellular carcinoma (HCC). Presently, we isolated and purified one polysaccharide from this mushroom. This study aimed to investigate the effects of SP1 on tumor growth and metastasis in a HCC xenograft model and explore its possible mechanism of action. Our results showed that SP1 not only significantly inhibited the proliferation of SMMC-7721 cells in vitro at the concentration ranging from 0 to 800 ?g/ml but also suppressed the HCC tumor growth and metastatic nodules to the lung in SMMC-7721-bearing mice by oral administration at three doses of 30, 60, and 120 mg/kg. Concomitantly, immunohistochemistry analysis of tumor tissues identified that SP1 administration at three doses significantly inhibited the in vivo cancer cell proliferation and microvessel density (MVD) formation, evidenced by a low proliferating cell nuclear antigen (PCNA) and CD34 expression, but increased the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive cells. Keeping in line with this observation, SP1 treatment decreased serum matrix metalloproteinase (MMP) 2 and vascular endothelial growth factor (VEGF) levels, downregulated the protein expression of hypoxia-inducible factor (HIF)-1alpha, VEGF, MMP2, bcl-2, N-cadherin, signal transducer and activator of transcription 3 (STAT3), and metadherin (MTDH), and upregulated bax and NE-cadherin protein expression in tumor tissues. Taken together, our data suggest that SP1 appears to be a promising chemopreventive agent for the tumorigenesis and metastasis in patients with HCC, especially at advanced stage
Frequently rearranged in advanced T-cell lymphomas-1 demonstrates oncogenic properties in prostate cancer
No full textMetal-organic Vapor Phase Epitaxy Growth and Characterization of InAlGaN Epilayers
No full textIn order to improve crystal quality for growth of quaternary InAlGaN, a series of InAlGaN films were grown on GaN buffer layer under different growth temperatures and carrier gases by low-pressure metal-organic vapor phase epitaxy. Energy dispersive spectroscopy (EDS) was employed to measure the chemical composition of the quaternary, high resolution X-ray diffraction (HRXRD) and photoluminescence (PL) technique were used to characterize structural and optical properties of the epilayers, respectively. The PL spectra of InAlGaN show with and without the broad-deep level emission when only N2 and a N2+H2 mixture were used as carrier gas, respectively. At pressure of 1.01×104 Pa and with mixed gases of nitrogen and hydrogen as carrier gas, different alloy compositions of the films were obtained by changing the growth temperature while keeping the fluxes of precursors of indium (In), aluminum (Al), gallium (Ga) and nitrogen (N2) constant. A combination of HRXRD and PL measurements enable us to explore the relative optimum growth parameters-growth temperature between 850℃ and 870℃,using mixed gas of N2+H2 as carrier gas
Identification of Rubisco rbcL and rbcS in Camellia oleifera and Their Potential as Molecular Markers for Selection of High Tea Oil Cultivars
No full textTea oil derived from seeds of Camellia oleifera Abel. is a high-quality edible oil in China. This study isolated full-length cDNAs of Rubisco subunits rbcL and rbcS from C. oleifera. The rbcL has 1,522 bp with a 1,425 bp coding region, encoding 475 amino acids; and the rbcS has 841 bp containing a 528 bp coding region, encoding 176 amino acids. The expression level of the two genes, designated as Co-rbcL and Co-rbcS, was determined in three C. oleifera cultivars: Hengchong 8, Xianglin 1, and Xianglin 14 whose annual oil yields were 546.9, 591.4, and 657.7 kg ha-1, respectively. The Co-rbcL expression in ‘Xianglin 14’ was significantly higher than ‘Xianglin 1’, and ‘Xianglin 1’ was greater than ‘Hengchong 89’. The expression levels of Co-rbcS in ‘Xianglin 1’ and ‘Xianglin 14’ were similar but were significantly greater than in ‘Hengchong 89’. The net photosynthetic rate of ‘Xianglin 14’ was significantly higher than ‘Xianglin 1’, and ‘Xianglin 1’ was higher than ‘Hengchong 89’. Pearson’s correlation analysis showed that seed yields and oil yields were highly correlated with the expression level of Co-rbcL at P 0.001 level, and the expression of Co-rbcS was correlated with oil yield at P 0.01 level. Net photosynthetic rate was also correlated with oil yields and seed yields at P 0.001 and P 0.01 levels, respectively. Our results suggest that Co-rbcS and Co-rbcL in particular could potentially be molecular markers for early selection of high oil yield cultivars. In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency
A bifunctional antibody targeting PD-1 and TGF-β signaling has antitumor activity in combination with radiotherapy and attenuates radiation-induced lung injury
No full textRadio-immunotherapy has antitumor activity but also causes toxicity, which limits its clinical application. JS-201 is a dual antibody targeting PD-1 and TGF-β signaling. We investigated the antitumour effect of JS-201 combined with radiotherapy and the effect on radiation-induced lung injury (RILI). Different tumor models were established to detect the antitumor effects of the combination of JS-201 and RT, and RILI models were established to observe the effects of JS-201. Transcriptome sequencing showed that JS-201 optimized the TME by inhibiting extracellular matrix formation and angiogenesis. Combining JS-201 with radiotherapy further increased the inflammatory response and immune infiltration and showed great abscopal effects in LLC-luc models. Single-cell sequencing demonstrated that JS-201 reduced fibroblast proliferation by inhibiting the TGF-β/Smad pathway and the release of neutrophil extracellular traps mediated by ROS, thereby relieving radiation-induced pulmonary fibrosis. In conclusion, the JS-201 and radiotherapy combination enhances antitumor effects while mitigating acute and chronic RILI, and it may have potential for translational investigation as a cancer treatment strategy
Exploring Novel Quantum Materials Under Pressure
No full textThesis (Ph.D.)--Michigan State University. Chemistry - Doctor of Philosophy, 2025The pursuit of novel quantum materials lies at the forefront of condensed matter research, driven by the search for emergent phenomena such as unconventional superconductivity, topological phases, and exotic magnetism. Among the various tuning parameters, external pressure offers a clean and effective approach to explore uncharted regions of phase space by modulating interatomic distances, electronic bandwidths, and crystal symmetry, often stabilizing metastable or entirely new quantum states inaccessible at ambient conditions. This dissertation leverages high-pressure synthesis in combination with single crystal X-ray diffraction and detailed physical property measurements to discover and characterize previously unknown quantum materials with emergent electronic and magnetic behaviors. Key results include: (1) the synthesis of Ruddlesden\u2013Popper strontium iridates Srn+1IrnO3n+1, unveiling non-centrosymmetric Sr2IrO4 with spin-canted antiferromagnetism and a high-symmetry tetragonal SrIrO3; (2) the identification of a hidden polymorph and a pressure-induced structural transition in Ruddlesden\u2013Popper bilayer nickelate La3Ni2O7, offering insight into the structural landscape of nickelate superconductors. Collectively, these results underscore the power of high-pressure techniques in discovering new quantum materials and tuning their ground states through symmetry control and chemical bonding. This work lays the groundwork for future exploration of pressure-temperature phase diagrams and provides a framework for understanding structure-property relationships in strongly correlated systems.Description based on online resource. Title from PDF t.p. (Michigan State University Fedora Repository, viewed ).Includes bibliographical references
Shrub and herb diversity, litter quality, MBC, bulk density, soil organic stocks and stability in Mixed Forests of the Loess Plateau, China (2023-2024)
No full textThis dataset was obtained through field sampling conducted at two key research stations: the Ansai Comprehensive Soil and Water Conservation Experimental Station (Chinese Academy of Sciences) and the Shanxi Jixian National Forest Ecosystem Observation and Research Station. It encompasses ecological parameters from mixed forests in China's Loess Plateau during 2023-2024, including shrub and herbaceous plant diversity, litter quality (C/N ratio, biomass), bulk density, organic carbon stocks, and stability indices. These data provide fundamental support for elucidating plant-soil interactions and assessing carbon sequestration potential in afforested ecosystems, contributing to research on sustainable forest management and climate change mitigation
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