10,021 research outputs found
Monitoring Cl- movement in single cells exposed to hypotonic solution
No full textSelf-referencing ion--selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1-3 microm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparing potentials (VN) measured in 100 and 10 mM NaCl (deltaVN = 57 +/- 2 mV), but was slightly greater than ideal when comparing 1 and 10 mM NaCl (deltaVN = 70 +/- 3 mV). The response was also linear in the presence of 1 mM glutamate, gluconate, or acetate, 10 microM tamoxifen, or 0.1, 1, or 10 mM HEPES at pH 7.0. The ISE was approximately 3 orders of magnitude more selective for Cl- over glutamate or gluconate but less than 2 orders of magnitude move selective for Clover bicarbonate, acetate, citrate or thiosulfate. As a result this ISE is best described as an anion sensor. The ISE was 'poisoned' by 50 microM 5-nitro-2-(3phenylpropyl-amino)-benzoic acid (NPPB), but not by tamoxifen. An outward anion efflux was recorded from cells challenged with hypotonic (250 +/- 5 mOsm) solution. The increase in efflux peaked 7-8 min before decreasing, consistent with regulatory volume decreases observed in separate experiments using a similar osmotic protocol. This anion efflux was blocked by 10 microM tamoxifen. These results establish the feasibility of using the modulation of electrochemical, anion-selective, electrodes to monitor anions and, in this case, chloride movement during volume regulatory events. The approach provides a real-time measure of anion movement during regulated volume decrease at the single-cell leve
A GSMBE grown GaInP/GaAs narrow base DHBT exhibiting N-shape negative differential resistance with variable peak-to-valley current ratio up to 1?107 at room temperature
No full textUltra-high speed 1μm V-gate GaAs MESFET with cutoff frequency up to 47GHz by 2D simulation
No full textA Generalization of Ostrowski Integral Inequality for Mappings Whose Derivatives Belong to L₁ [a,b] and Applications in Numerical Integration
No full textA generalization of Ostrowski integral inequality for mappings whose derivatives belong to L₁[a,b], and applications for general quadrature formulae are given
Midpoint Type Rules from an Inequalities Point of View
No full textThe article investigates interior point rules which contain the midpoint as a special case, and obtains explicit bounds through the use of a Peano kernel approach and the modern theory of inequalities. Thus the simplest open Newton-Cotes rules are examined. Both Riemann-Stieltjes and Riemann integrals are evaluated with a variety of assumptions about the integrand enabling the characterisation of the bound in terms of a variety of norms. Perturbed quadrature rules are obtained through the use of Grüss, Chebychev and Lupaş inequalities, producing a variety of tighter bounds. The implementation is demonstrated through the investigation of a variety of composite rules based on inequalities developed. The analysis allows the determination of the partition required that would assure that the accuracy the result would be within a prescribed error tolerance. It is demonstrated that the bounds of the approximations are equivalent to those obtained from a Peano kernel that produces Trapezoidal type rules
Siles, S.S., Letter to Mary Knight, September 20, 1863
Full text linkStiles, S.S., Letter to Mary Knight, September 20, 1863. MS/52 William Knight Civil War Letters, Box 1, Folder 3. Valdosta State University Archives and Special Collections. 1 PDF and 2 scans. Includes transcript.The letter, dated September 20, 1863, is from Camp Lee and addressed to Mary. The author, identified as S.S. Siles, expresses relief that William has returned home and hopes for his quick recovery. Siles mentions the difficulties faced by women who visit camps to bring their sick husbands home, noting that officers rarely pay them any attention.
Siles laments the poor condition of the tents, which are old and worn, failing to provide adequate shelter from rain. He mentions that the Quarter Master tried but failed to secure new tents in Savannah. The letter also touches on the precarious state of the Confederate army, suggesting that its future may hinge on an upcoming battle in Northern Georgia. Siles fears that a defeat could lead to mass desertions.
The letter describes the men as being very sickly, attributing this to their poor diet of only beef and cornmeal. Siles himself is not in good health, suffering from chest pains and fevers, but continues to perform his duties as an acting Orderly Sergeant. He urges Mary to tell William to write to him and to keep him updated with news
Clinical outcome of heart transplantation :experience at the National Taiwan University Hospital.
No full text
Dataset for Reconfigurable phase-change photomask for grayscale photolithography
No full textData set to support:
Wang, Q. et al (2017). Reconfigurable phase-change photomask for grayscale photolithography, Applied Physics Letters</span
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