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    Asarum ampulliflorum (Aristolochiaceae), a new species from Taiwan

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    Lu, Chang-Tse, Wang, Jenn-Che (2014): Asarum ampulliflorum (Aristolochiaceae), a new species from Taiwan. Phytotaxa 184 (1): 46-52, DOI: 10.11646/phytotaxa.184.1.5, URL: http://dx.doi.org/10.11646/phytotaxa.184.1.

    FIGURE 2 in Asarum ampulliflorum (Aristolochiaceae), a new species from Taiwan

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    FIGURE 2. Geographical distribution of Asarum ampulliflorum C.–T. Lu & J.–C. Wang, A. asaroides Morr. et Decne., A. macranthum Hooker f., and A. satsumense F. Maekawa.Published as part of Lu, Chang-Tse & Wang, Jenn-Che, 2014, Asarum ampulliflorum (Aristolochiaceae), a new species from Taiwan, pp. 46-52 in Phytotaxa 184 (1) on page 49, DOI: 10.11646/phytotaxa.184.1.5, http://zenodo.org/record/514647

    FIGURE 1. Asarum ampulliflorum C.–T in Asarum ampulliflorum (Aristolochiaceae), a new species from Taiwan

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    FIGURE 1. Asarum ampulliflorum C.–T. Lu & J.–C. Wang (from holotype). A. Habit. B. Apical view of flower. C. Longitudinally dissected flower. D. Outer whorl stamens. E. Inner whorl stamens. F. Style and stigma. G. Dissected ovary. (drawn by C.–T. Lu)Published as part of Lu, Chang-Tse & Wang, Jenn-Che, 2014, Asarum ampulliflorum (Aristolochiaceae), a new species from Taiwan, pp. 46-52 in Phytotaxa 184 (1) on page 48, DOI: 10.11646/phytotaxa.184.1.5, http://zenodo.org/record/514647

    Species Identification Using Sequences of the Trnl Intron and the Trnl- Trnf Igs of Chloroplast Genome among Popular Plants in Taiwan

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    Forensic botanical comparison can be hampered by the lack of appropriate DNA databases. While DNA sequence databases for many mitochondrial loci have been established for the identification of animal species, less is known regarding the genomes of plants. We report on the use of the trnL intron and the trnL-trnF intergenic spacer (IGS) in the chloroplast genome and establish a DNA sequence database for plant species identification. The DNA sequences at these two loci from commonly encountered plants, including monocots and dicots, were aligned to establish a DNA database of local plants. The database comprises 373 individual sequences representing 80 families, 206 genera and 269 species. These plant species can be grouped to species level using both sequence and length polymorphisms at these loci. To validate the database for future forensic purposes, we sequenced 20 blind samples and searched the local database and the databases of GenBank and EMBL. Fifteen of these 20 samples used in blind trial testing matched their respective species from our local DNA database but only 6 matched species registered in the GenBank and EMBL databases. The sequences of two species used in the blind trial did not match any sequence registered in any of these databases. Cluster analysis was performed to demonstrate the family and genus distribution of samples. Neighbor-joining trees of the two DNA regions from 70 samples of the local database and 10 of the species used in the blind trials were constructed and clustered to both family and genus. The bootstrap values of the trnL intron were higher than most of those of the trnL-trnF IGS. The sequence database described in this study can be used to identify plant species using DNA sequences of the trnL intron and trnL-trnF IGS of chloroplast genome and illustrates its value in plant species identification

    Bidens identification using the noncoding regions of chloroplast genome and nuclear ribosomal DNA

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    Bidens pilosa L. is a plant producing barbed fruits which, due to its method of seed dispersal, is commonly found during forensic investigations. In Taiwan there are three varieties of the species, radiata, minor and pilosa. Fragments of these three varieties are difficult to differentiate by traditional morphological characteristics and until now little is known of their genetic composition. To discover genetic polymorphisms among these varieties, five loci within the nuclear and chloroplast genomes were screened. A total of 161 specimens were used in this study comprising different geographical populations. Seven samples of Bidens biternata were included as an out-group control. DNA fragments of all samples at the trnL intron and trnL-trnF IGS loci of the chloroplast genome, internal transcribed spacer (ITS1 and ITS2) and the 5.8S of nuclear ribosomal DNA (nrDNA) were amplified and sequenced. There were 3, 4, 20, 12 and 9 sequence types at these five loci, respectively. The sequence types for any locus of trnL intron, ITS1, ITS2 and 5.8S were found to be useful markers to identify Bidens biternata and B. pilosa. The resulting 84 haplotypes at the 5 loci could differentiate the var. radiata from the varieties of B. pilosa with only the exception of 1 type. The genetic polymorphisms can be used when comparing botanical remains to identify the variety of B. pilosa present at a crime scene

    [[alternative]]A Taxonomic Study of Commelinaceae of Taiwan

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    [[abstract]]In the course of a taxonomic revision of the Commelinaceae in Taiwan, 25 species in eight genera were recognized, including a new species and anew variety of Murdannia and a new record, Cyanotis axillaris (L.)Sweet.Murdannia triquetra (Wall.) Br(ckn. was synonymized with Murdannia keisak(Hassk.) Hand.- Mazz. A new combination, Belosynapsis kawakamii, was madeof Cyanotis kawakamii Hayata. In this study, seed morphology was shown to be important in distinguishingplants of Taiwan Commelinaceae. Morphology of petals, stamens (both fertile andsterile), and fruits are diagnostic at generic and sometimes specific level.Plant habit, roots, stems, leaves, and inflorescence types are often useful indistinguishing related species. Pollen size, exine sculpture, and sulcalmorphology are useful in sepannia, in which pollenf all species are similar.All but a number of populations of Belosynapsis ciliata and Commelina diffusaexamined have stainable pollen. Studies of chromosome cytology reveals that Taiwanese Amischotolype isbased on n = 9. Belosynapsis has a basic chromosome number of n = 13, althoughB. ciliata has n = 18. Cyanotis is based on n = 12. Commelina has n = 15 in allbut Co. benghalensis and Co. communis, which have n = 11. Murdannia has n= 10,with possibly derived aneuploid chromosome numbers in several species. Polliahas n = 16. Rhopalephora is based on n = 29. Tetravalents were observed inmeiosis of Belosynapsis ciliata and Floscopa scands. First reports on chromosomenumbers include Belosynapsis kawakamii (n = 26), Murdannia angustifolia(n = 19, 20), M. bracteata (n = 9), Murdannia taiwanensis (n = 28), and Murdanniataiwanensis var. lilungensis (n = 28). Counts of n = 18 for Amischotolype hispida,n = 10 II + 1 IV for Floscopa scandens, n = 21 for Murdannia edulis, and n = 16for Pollia miranda disagree with previous reports.

    A taxonomic study of Camellia brevistyla and C. tenuiflora (Theaceae) based on phenetic analyses.

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    [[sponsorship]]植物暨微生物學研究所[[note]]已出版;[SCI];有審查制度;具代表性[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Drexel&SrcApp=hagerty_opac&KeyRecord=1817-406X&DestApp=JCR&RQ=IF_CAT_BOXPLOT[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=RID&SrcApp=RID&DestLinkType=FullRecord&DestApp=ALL_WOS&KeyUT=00030457740001
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