10,877 research outputs found
HC-Pro interacts with XRN4 and inhibits XRN4 activity.
No full text(A) BiFC assays to confirm the HC-Pro and NbXRN4 interaction in H2B-RFP N. benthamiana leaves at 32 hpi. HC-Pro and NbXRN4 were fused with YN and YC. Yellow fluorescence (green) was observed in the cells co-expressing YN-HC-Pro+YC-NbXRN4 or YC-HC-Pro+YN-NbXRN4. Co-expression of YN-HC-Pro+YC-NbDCP1 or YC-HC-Pro+YN-NbDCP1 did not result in any detectable fluorescence in H2B-RFP N. benthamiana leaves at 32 hpi, which revealed that HC-Pro did not interact with NbDCP1. Bars = 25 μm. (B) Co-localization analysis of HC-Pro-CFP and YFP-NbXRN4 in N. benthamiana leaves at 32 hpi. Panel I: HC-Pro-CFP was expressed alone, Panel II: YFP-NbXRN4 was expressed alone, Panel III: HC-Pro-CFP and YFP-NbXRN4 were expressed together. Bars = 25 μm. (C) Y2H assays for AtXRN4 and HC-Pro. Y2H Gold yeast strains co-transformed with the indicated plasmids were subjected to 10-fold serial dilutions and plated on SD/-Trp, -Leu, -His, -Ade medium to identify protein interactions at 3 days after transformation. Cells co-transformed with AD-T7-T+BD-T7-53 serve as positive controls; cells co-transformed with AD-HC-Pro and the empty BD, or the empty AD and BD-AtXRN4 are negative controls. (D) BiFC assays revealed that HC-Pro interacted with AtXRN4 in H2B-RFP N. benthamiana leaves at 32 hpi. P3N-PIPO and AtXRN4 served as a negative control for the protein-protein interaction assay. Bars = 25μm. (E) Phenotypes of Col-0, xrn4 mutant, and transgenic Arabidopsis plants transformed with 35S:Myc-AtXRN4, 35S:HC-Pro-CFP-1, 35S:HC-Pro-CFP-2 and 35S:HC-Pro-CFP-1/35S:Myc-AtXRN4 at 20 days after sowing. 35S:HC-Pro-CFP-1/35S:Myc-AtXRN4 plants were obtained by genetic crosses between 35S:HC-Pro-CFP-1 and 35S:Myc-AtXRN4 Arabidopsis plants. T2 generation plants were used in the above experiments. The confirmation of xrn4 mutant, transgenic plants expressing 35S:Myc-AtXRN4, 35S:HC-Pro-CFP-1, 35S:HC-Pro-CFP-2 and 35S:HC-Pro-CFP-1/35S:Myc-AtXRN4 was shown in S7 Fig and S10 Fig. (F, G, H) qRT-PCR analysis of AtEBF1, AtRAP2.4, AtNMT expression in Col-0, xrn4 mutant, and transgenic plants carrying 35S:Myc-AtXRN4, 35S:HC-Pro-CFP-1 and 35S:HC-Pro-CFP-1/35S:Myc-AtXRN4 at 20 days after sowing (F), in Col-0 and xrn4 mutant leaves infiltrated by empty vector (Vec) and HC-Pro at 3 dpi (G), and in Col-0 newly emerged leaves of mock- or TuMV-infected plants 12 dpi (H). AtActinII gene was used as an internal control. The data were analyzed using Student’s t test and asterisks denote significant differences between treatments (* P P <0.01).</p
Petrocosmea melanophthalma, a New Species in Section Deianthera (Gesneriaceae) from Yunnan, China
No full textPet rocosmea melanophthalma Huan C. Wang, Z. R. He & Li Bing Zhang, an attractive new species (Gesneriaceae, Petrocosmea sect. Deianthera W. T. Wang) from Yunnan, China, is described and illustrated. It is similar to P. kerrii Craib in the zygomorphic calyx and brevirostrate anther. The new species differs from the latter by its peltate leaves and the blue corolla with two striking black spots on the tube, as well as the geniculate filaments of the stamens that thicken above the middle. Petrocosmea melanopthalma was found on basalt cliffs in evergreen broad-leaved forest on Mopan Shan
Li Maneuver for Geotropic Right HC-BPPV, Canalithiasis
No full textThe Li maneuver is used to treat horizontal canal, canalithiasis. When compared to the Gufoni maneuver, the Li maneuver was as effective to treat HC-BPPV and there was no significant difference between the maneuvers; however, the Li Maneuver may take less time to complete. 1. The patient starts in a supine position. 2. The patient rolls 90 degrees towards the affected ear and this position is held until the sensation of vertigo subsides. 3. The patient then rolls rapidly to the opposite side (180 degrees towards the unaffected ear) and this position is held for two minutes.Zhao P, Li J, Ding Y, Wang Y, Zou S. Li maneuver for geotropic horizontal canal benign paroxysmal positional vertigo (HC-BPPV) -A better choice. American journal of otolaryngology. 2021;42(5):103-132. https://dx.doi.org/10.1016/j.amjoto.2021.103132
Sequencing and amplified restriction fragment length polymorphism analysis of ribonucleotide reductase large subunit gene of the white spot syndrome virus in blue crab (Callinectes sapidus) from American coastal waters.
No full textModulation of swarming and virulence by fatty acids through RsbA protein in Proteus mirabilis.
No full textIdentification of icp11, the most highly expressed gene of shirmp white spot syndrme virus
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Flux pinning in superconducting YBCO thin films with an artificial magnetic pinning lattice
No full textAngular dependence of tunneling magneto resistance in La0.7Sr0.3MnO3 step-edge junctions
No full textIdentification and characterization of an organic solvent tolerance gene in Helicobacter pylori.
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