35 research outputs found

    FIGURE 3 in Tuber qujingense and T. songlu, two new species from Yunnan, China

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    FIGURE 3. Tuber qujingense (HKAS 95823, type) A. An ascoma and its gleba; B. Dermatocystidia; C. Peridium section; D, E. Light micrograph (LM) of ascospores; F. SEM photo of an ascospore. Photos by: Shanping Wan.Published as part of Wan, Shanping, Liu, Jianwei, Huang, Lanlan, Qin, Xiaomin, Liu, Wei & Yu, Fuqiang, 2021, Tuber qujingense and T. songlu, two new species from Yunnan, China, pp. 248-256 in Phytotaxa 527 (4) on page 252, DOI: 10.11646/phytotaxa.527.4.2, http://zenodo.org/record/576617

    Tuber qujingense S. P. Wan 2021, sp. nov.

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    <i>Tuber qujingense</i> S. P. Wan, <i>sp. nov.</i> (Fig. 3) <p>MycoBank: MB 839733</p> <p> Typification: CHINA. Yunnan Province, Huize County (18.103°E, 23.26°N), in humic soil under a pure <i>Pinus armandii</i> forest, at about 2400 m, 12 August 2016, <i>wsp721</i>, HKAS 95823 (GenBank Acc. No.: ITS = KX904885, LSU = KY 013659).</p> <p> Diagnosis: <i>Tuber qujingense</i> differs from related species by its greyish white ascomata, brown snowflake-shaped gleba, prosenchymatous peridium, fusiform ascospores and 1–4 spored asci.</p> <p>Etymology: Refers to the location of the type collection.</p> <p> Description: <i>Ascoma</i> 2.5 cm in diam, subglobose or irregular, greyish white when fresh, becoming brown when dried. <i>Peridium</i> 200–500 µm thick, smooth to pubescent, one layer, prosenchymatous, composed of big, subglobose to subangular cells, (1–) 2–33 (–34.5) × (1–) 1.5–22 (–33) µm, light earthy yellow. <i>Gleba</i> solid, brownish purple when mature, marbled with white veins, composed of hyaline, interwoven, thin-walled hyphae, 1.5–6 µm, and cylindrical, inflated hyphae 2.8–50 × 2.8–43 µm. <i>Dermatocystidia or setae</i>, straight or bent, obtuse or apiculate at the tip, up to 110 µm long, 6.5 µm in diam, septate, hyaline to whitish. <i>Asci</i> (40–) 51–80 × (30–) 31–60 µm, globose to subglobose, pyriform, ellipsoid or irregular, hyaline, sessile or with a short stalk, thin-walled 1–2 µm thick, 1–4 spored. <i>Ascospores</i>, fusiform, ellipsoid, sometimes broadly ellipsoid, subglobose, hyaline when young, becoming brown at maturity; excluding the alveolate-reticulate ornamentation, in 1-spored asci (35–)37–48(–50) × (23–) 25–30(–32) µm (Q = 1.45–1.65), in 2-spored (22–) 28–40(–41) × (14–) 18-27 µm (Q = 1.41–1.7), in 3-spored (20–)3 8–18(–20) × 15–19(– 24) µm (Q = 1.23–1.75), and in 4-spored (17–) 20–34(–36) × 14–21(–22) µm (Q = 1.14–1.72); reticulum with 3–10 meshes along the spore length and 3–8 across. The alveolar walls up to 4.5–11 µm tall.</p> <p> <i>Distribution and habitat</i>: Hypogeous, in soil under pure stand of <i>P. armandii</i> in Yunnan Province, China. Known only from China.</p>Published as part of <i>Wan, Shanping, Liu, Jianwei, Huang, Lanlan, Qin, Xiaomin, Liu, Wei & Yu, Fuqiang, 2021, Tuber qujingense and T. songlu, two new species from Yunnan, China, pp. 248-256 in Phytotaxa 527 (4)</i> on pages 251-252, DOI: 10.11646/phytotaxa.527.4.2, <a href="http://zenodo.org/record/5766179">http://zenodo.org/record/5766179</a&gt

    Tuber songlu S. P. Wan 2021, sp. nov.

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    <i>Tuber songlu</i> S.P. Wan, <i>sp. nov.</i> (Fig. 4) <p>MycoBank: MB 839734</p> <p> Typification: CHINA. Yunnan Province, Huize County (29.103°E, 25.56°N), in humic soil under a pure <i>Pinus armandii</i> forest, at about 2415 m, 14 August 2016, <i>wsp695</i>, HKAS 95771 (GenBank Acc. No.: ITS = KX904883, LSU = KY 013656).</p> <p> Diagnosis: <i>Tuber songlu</i> differs from other species by its whitish ascomata, dense spine-like dermatocystidia, ellipsoid ascospores and 1–4 spored asci.</p> <p> <i>Etymology</i>: Refers to the local name of the fungus.</p> <p> Description: <i>Ascomata</i> 1–2 cm in diam, globose to subglobose or irregular, white, light reddish brown when fresh, becoming brown when dried. <i>Peridium</i> 130–500 µm thick, densely pubescent, one layer, prosenchymatous, composed of subglobose to subangular cells, (1–) 1.5–18(–23) × (1–) 1.5–7(–8.5) µm, hyaline. <i>Gleba</i> solid, brown when mature, marbled with brownish yellow veins, composed of hyaline, interwoven, thin-walled cylindrical hyphae, 1.5–8.5 µm broad at the septa, and interwoven, inflated hyphae 2–8.5 µm broad. <i>Dermatocystidia</i>, straight or bent, obtuse or apiculate at the tip, up to 230 µm long, 7 µm in diam, multiseptate, hyaline to whitish. <i>Asci</i> (63–) 72–96 × 54–75 (–81) µm, globose to subglobose, pyriform, ellipsoid or irregular, hyaline, sessile or with a short stalk, thin-walled 1–2 µm thick, 1–4 spored. <i>Ascospores</i>, ellipsoid, sometimes broadly ellipsoid, subglobose, hyaline when young, becoming brown at maturity; excluding their alveolate-reticulate ornamentation, in 1-spored asci (41–) 42.5–51.5 (–56) × (26.5–) 32.5–40 (–41) µm (Q = 1.10–1.70), in 2-spored (22–) 24–42 (–46) × (18–) 19–34 µm (Q = 1.10–1.51), in 3-spored (20–) 26–33 (–35) × 19 (–21)–29 (–30) µm (Q = 1.13–1.24), and in 4-spored (19–) 20–27.5 (–29) × 15–17 (–27) µm (Q = 1.1–1.2); reticulum with 3–8 meshes along the spore length and 3–8 across. The alveolar walls up to 1–7 µm tall.</p> <p> <i>Distribution and habitat</i>: Hypogeous, in soil under a pure forest of <i>Pinus armandii</i> in Yunnan Province, China. Known only from China.</p> <p> <i>Other material examined</i>: CHINA. Yunnan Province, Province, Huize County, in soil under a pure forest of <i>P. armandii</i>, December 2016, <i>wsp701</i>, HKAS 95777 (GenBank Acc. No.: ITS = KX904884, LSU = KY 013657); <i>wsp749</i>, HKAS 95851 (GenBank Acc. No.: ITS = KX904886, LSU = KY 013658).</p>Published as part of <i>Wan, Shanping, Liu, Jianwei, Huang, Lanlan, Qin, Xiaomin, Liu, Wei & Yu, Fuqiang, 2021, Tuber qujingense and T. songlu, two new species from Yunnan, China, pp. 248-256 in Phytotaxa 527 (4)</i> on pages 253-254, DOI: 10.11646/phytotaxa.527.4.2, <a href="http://zenodo.org/record/5766179">http://zenodo.org/record/5766179</a&gt
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