386 research outputs found
Olive Inman, (1886-1946), purchased by Mr. Norman C. Inman on October 27, 1948.
No full textDocuments regarding the double headstone for Olive Inman, (1886-1946), buried with Charles McCoy, (1891-1931), purchased by Mr. Norman C. Inman. The marker was placed at Forest Cemetery, Lot 9, Section C-1, in Toledo, Ohio. The stone is made of Barre with Sandblast letters
Hiran Inman, (1852-1947), purchased by Mrs. Ethel M. Robison on August 14, 1953.
No full textDocuments regarding the headstone for Hiran Inman, (1852-1947), purchased by Mrs. Ethel M. Robison. The marker was placed at Toledo Memorial Park Cemetery in Sylvania, Ohio. The stone is made of Barre rock of ages granite with Sandblast letters. Rubbings is included
A Study of Henry Inman, 1837-1899
No full textHenry Inman, soldier, newspaperman, social historian, author of articles, short stories and books on frontier life, was a man of eccentric habits and various talents. A man of impulsive action and limited imagination, be completed and published some eight books dealing, for the most part, with the land he knew and loved, the American West. Divided into tour major sections, the thesis initially covered Inman’s lineal descent, education, intellectual heritage and temperament; the second part treats of his military career, his problems in dealing with a job for which he was entirely unfitted, his various courts martial and subsequent dismissal from the service of the United States Army. The third section reviews his life after his cashiering in 1872 the various literary contributions, both as a newspaper editor and contributor, and as a novelist and chronicler. It pursues investigation of Inman throughout the remainder of his life, and ends with his death and burial. The fourth section concerns itself primarily with the themes which recur throughout his writing and with the plots around which he builds his stories of fictionalized prose. The conclusion will allude rather briefly to the effect of his life and works from the standpoint of regional value
Co-regulation of primary mouse hepatocyte viability and function by oxygen and matrix
No full textAlthough oxygen and extracellular matrix cues both influence differentiation state and metabolic function of primary rat and human hepatocytes, relatively little is known about how these factors together regulate behaviors of primary mouse hepatocytes in culture. To determine the effects of pericellular oxygen tension on hepatocellular function, we employed two methods of altering oxygen concentration in the local cellular microenvironment of cells cultured in the presence or absence of an extracellular matrix (Matrigel) supplement. By systematically altering medium depth and gas phase oxygen tension, we created multiple oxygen regimes (hypoxic, normoxic, and hyperoxic) and measured the local oxygen concentrations in the pericellular environment using custom-designed oxygen microprobes. From these measurements of oxygen concentrations, we derived values of oxygen consumption rates under a spectrum of environmental contexts, thus providing the first reported estimates of these values for primary mouse hepatocytes. Oxygen tension and matrix microenvironment were found to synergistically regulate hepatocellular survival and function as assessed using quantitative image analysis for cells stained with vital dyes, and assessment of secretion of albumin. Hepatocellular viability was affected only at strongly hypoxic conditions. Surprisingly, albumin secretion rates were greatest at a moderately supra-physiological oxygen concentration, and this effect was mitigated at still greater supra-physiological concentrations. Matrigel enhanced the effects of oxygen on retention of function. This study underscores the importance of carefully controlling cell density, medium depth, and gas phase oxygen, as the effects of these parameters on local pericellular oxygen tension and subsequent hepatocellular function are profound.National Institutes of Health (U.S.) (Grant P50-GM068762-08)National Institutes of Health (U.S.) (Grant R01-EB010246-04)National Institutes of Health (U.S.) (Grant R01-ES015241)National Institutes of Health (U.S.) (Grant P30-ES002109
Challenges in Using Cultured Primary Rodent Hepatocytes or Cell Lines to Study Hepatic HDL Receptor SR-BI Regulation by Its Cytoplasmic Adaptor PDZK1
No full textBackground:
PDZK1 is a four PDZ-domain containing cytoplasmic protein that binds to a variety of membrane proteins via their C-termini and can influence the abundance, localization and/or function of its target proteins. One of these targets in hepatocytes in vivo is the HDL receptor SR-BI. Normal hepatic expression of SR-BI protein requires PDZK1 - <5% of normal hepatic SR-BI is seen in the livers of PDZK1 knockout mice. Progress has been made in identifying features of PDZK1 required to control hepatic SR-BI in vivo using hepatic expression of wild-type and mutant forms of PDZK1 in wild-type and PDZK1 KO transgenic mice. Such in vivo studies are time consuming and expensive, and cannot readily be used to explore many features of the underlying molecular and cellular mechanisms.
Methodology/Principal Findings:
Here we have explored the potential to use either primary rodent hepatocytes in culture using 2D collagen gels with newly developed optimized conditions or PDZK1/SR-BI co-transfected cultured cell lines (COS, HEK293) for such studies. SR-BI and PDZK1 protein and mRNA expression levels fell rapidly in primary hepatocyte cultures, indicating this system does not adequately mimic hepatocytes in vivo for analysis of the PDZK1 dependence of SR-BI. Although PDZK1 did alter SR-BI protein expression in the cell lines, its influence was independent of SR-BI’s C-terminus, and thus is not likely to occur via the same mechanism as that which occurs in hepatocytes in vivo.
Conclusions/Significance:
Caution must be exercised in using primary hepatocytes or cultured cell lines when studying the mechanism underlying the regulation of hepatic SR-BI by PDZK1. It may be possible to use SR-BI and PDZK1 expression as sensitive markers for the in vivo-like state of hepatocytes to further improve primary hepatocyte cell culture conditions.National Institutes of Health (U.S.) (Grant HL052212)National Institutes of Health (U.S.) (Grant HL066105)National Institutes of Health (U.S.) (Grant ES015241)National Institutes of Health (U.S.) (Grant GM068762
FIGURE 14 in Three new ghost moths of the genus Oxycanus Walker, 1856 from Australia (Lepidoptera: Hepialidae)
No full textFIGURE 14. Inman Valley, Fleurieu Peninsula, South Australia. Highly degraded habitat of Oxycanus ephemerous sp. n., ©Ethan P. Beaver.Published as part of Beaver, Ethan P., Moore, Michael D., Velasco-Castrillón, Alejandro & Stevens, Mark I., 2020, Three new ghost moths of the genus Oxycanus Walker, 1856 from Australia (Lepidoptera: Hepialidae), pp. 351-374 in Zootaxa 4732 (3) on page 371, DOI: 10.11646/zootaxa.4732.3.1, http://zenodo.org/record/366712
An Oral History Interview of Robert Inman Johnson
No full textTranscribed from Arch CA 11-12 by Michele Fowler
Book Review: Immersive Media and Books 2020: New Insights About Book Pirates, Libraries and Discovery, Millennials, and Cross-Media Engagement: Before and During COVID
No full textBooks exist within a connected media ecosystem, but few consumer behavior and experience studies capture the relationships between books and other media forms. In Immersive Media & Books 2020, Drs. Rachel Noorda and Kathi Inman Berens from Portland State University explore crossmedia consumer behavior for books, video games, and TV/movies—capturing behaviors both before and during COVID-19. The highlights of the report are highly distributed word-of-mouth discovery, the importance of author brand and genre, avid book engagement of Black and Latinx millennials, context-agnostic book discovery, cross-media engagement and discovery, multidimensional identities and behaviors of book pirates, multitasking as a feature of contemporary book consumption, and libraries as tools of discovery
Development of a high throughput 3D perfused liver tissue bioreactor
No full textThesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006.Includes bibliographical references (p. 125-127).This thesis describes the development of a device designed for culturing liver tissue in a 3D perfused environment. Cells form tissue inside miniature channels of a scaffold, and the tissue is perfused with culture medium to create a culture microenvironment that has previously been described by the Griffith lab. In order to support this microenvironment, the reactor needs a pumping system, reservoirs and a controller. Previously, these have all been stand-alone components. This work focuses on the development of a new, integrated culture system. This system integrates 12 reactor microenvironments, reservoirs and pumping systems onto a single plate with a configuration modeled after standard multi-well plates. Each of the 12 bioreactor units utilize pneumatic pumps driven by a single external controller. This design offers substantial advantages over previous systems as it is far more user-friendly and can be used in a higher throughput capacity. The thesis describes the design and fabrication of the reactor and controller, including several models that were used during the development process. It also offers mechanical and biological characterizations of the device.by Samuel Walker Inman.S.M
Fe nano-particle coatings for high temperature wear resistance
No full textOxidational wear continues to present an economic challenge for the replacement of components subject to high temperature fretting and sliding contacts in applications such as gas turbine engines. At elevated temperatures, low friction oxide ‘glaze’ layers can form and act as an interface between the contact and the substrate material. Whilst desirable, the glaze is formed from wear debris and often consumes the underlying substrate material. In order to induce rapid formation of low friction oxide layers without a severe ‘running-in’ period, nano particles of Fe in the range 5-10nm were deposited on ground flat ended pin and plate 080M40 substrates using a terminated gas condensation PVD process, to a thickness of 600nm. Coatings were tested in a reciprocating geometry at a fixed stroke length of 0.4mm, frequency of 31Hz and 40N normal load (1MPa contact stress) and at ambient, 300°C and 540°C. At ambient temperature the coated surfaces exhibited higher friction but lower wear compared to the uncoated substrates, whereas at elevated temperatures, the coated surfaces exhibited slightly lower steady state dynamic friction coefficients, and minimal changes in wear depth after a short incubation period. SEM of the worn surfaces indicated that hard oxide plateaus were responsible for the load bearing contact area at elevated temperatures. Cross sectional FIB, TEM and SIMS confirmed that at elevated temperatures, the nano-particle coating induced rapid formation of a nano-crystalline porous surface oxide film of mixed composition which protected the substrate from severe wear during the running-in period
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