44 research outputs found
The compact mitogenome of Ceratocystiopsis pallidobrunnea
No full textCeratocystiopsis is a fungal genus that has been assigned to the Ophiostomatales, fungi known for their association with various bark beetles and other arthropods. The mitochondrial genome of Ceratocystiopsis pallidobrunnea has been characterized and compared with other members of the genus Ceratocystiopsis and Ophiostomatales. At 29 022 bp, the mitogenome of C. pallidobrunnea is the smallest reported so far for this genus. Gene arrangement was observed to be conserved for this group of fungi and mitogenome variation appears to be mostly due to the absence and presence of introns. The long-term goal is to apply mitogenomes to resolve taxonomic issues within the Ophiostomatales and within the various genera that comprise the Ophiostomataceae.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
The mitochondrial genome of Ophiostoma himal-ulmi and comparison with other Dutch elm disease causing fungi.
No full textThe mitochondrial genome of Ophiostoma himal-ulmi, a species endemic to the Western Himalayas and a member of the Dutch elm disease-causing fungi, has been sequenced and characterized. The mitochondrial genome was compared with other available genomes for members of the Ophiostomatales, including other agents of Dutch elm disease (Ophiostoma ulmi, Ophiostoma novo-ulmi subspecies novo-ulmi and Ophiostoma novo-ulmi subspecies americana) and it was noted that gene synteny is highly conserved and variability among members of the Dutch-elm disease-causing fungi is primarily due to the number of intron insertions. Among the Dutch elm disease-causing fungi examined, O. himal-ulmi has the largest mitochondrial genomes ranging from 94 934 bp to 111 712 bp due to the expansion of the number of introns.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
A study on the intron landscape of the cytochrome b gene, and mitochondrial-encoded N-acetyltransferase and ribosomal protein S3 genes of fungi in the subphylum Pezizomycotina
No full textMitochondrial genomes (mtDNAs) of fungi are highly variable in size. The variability can be partially attributed to the presence/absence of introns/intron-encoded open reading frames (ORFs) and genes. In order to study their effects on fungal mtDNAs, the potential intron insertion sites within the cytochrome b (cytb) gene and the distribution of mitochondrial-encoded N-acetyltransferase (NAT) and ribosomal protein S3 (rps3) genes were examined. The study on intron distribution in the cytb gene resulted in generation of an intron landscape. Many different intron/ORF arrangements were noted, potentially representing introns/ORFs at different stages of their life cycle. Based on the findings, a model of the life cycle of mobile composite elements was proposed. Both NAT and rps3 genes can be freestanding or intron-encoded. Based on phylogenetic analysis, NAT genes may have been imported in the mtDNA whereas the rps3 gene may have been imported into the nucleus in some fungi. The findings of the work suggest that the mtDNA is dynamic and contracts and expands due to factors including mobile introns/intron-encoded ORFs and genes.October 201
Eliza Frances Andrews.
No full textAndrews, an author, educator and botanist, was a colleague of Dr. Alvan Wentworth Chapman. She lived and studied in Alabama for several years and donated a large collection of plant specimens to the Alabama Department of Agriculture
Comparative mitogenomics of Leptographium procerum, Leptographium terebrantis and Leptographium wingfieldii, an invasive fungal species in Canadian forests
No full textLeptographium wingfieldii is a fungal associate of Tomicus piniperda (the pine shoot beetle) and pathogen of pines and this species is an agent of blue stain in sapwood on infected trees. This fungus was first reported from Europe and has been recently introduced to Canadian forests. Ten new mitogenomes have been sequenced and characterized, including seven strains of L. wingfieldii, two strains of L. procerum and one strain of L. terebrantis. The data were combined with other members of the Ophiostomatales collected from NCBI to gain more insight into the genetic diversity, evolution, and systematics of these fungi. The size of the studied mitogenomes of Leptographium species ranged from 41 kb to 126 kb with the number of potential mobile introns embedded within these mitogenomes ranging from 13 to 45. These data show that introns generate genetic diversity and confirms the contribution of mobile introns in genome expansion in Ophiostomatales fungi. This study also uncovered complex intron arrangements (twintrons) suggesting the potential of mobile introns generating complex ribozymes that may have implications in gene regulation.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
Mind and body; hypnotism and suggestion applied in therapeutics and education,
No full textMode of access: Internet
Three new active members of the I-OnuI family of homing endonucleases
No full textIn vitro characterization of three LAGLIDADG-type homing endonucleases (I-CcaI, I-CcaII and I-AstI) that belong to the I-OnuI family showed that they are functional homing endonucleases that cleave their respective cognate target sites. These endonucleases are encoded within group ID introns and appear to be orthologs that have inserted into three different mitochondrial genes, rns, rnl and cox3, respectively. The endonuclease activity of I-CcaI was tested using various substrates and its minimum DNA recognition sequence was estimated to be 26 nt. This set of homing endonucleases may provide some insight on how these types of mobile elements can migrate into new locations. This study provides additional endonucleases that can be added to the catalog of currently available HEs that may have various biotechnology applications.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
The complete mitochondrial genome of the Dutch elm disease fungus Ophiostoma novo-ulmi subspecies novo-ulmi.
No full textOphiostoma novo-ulmi, a member of the Ophiostomatales (Ascomycota) is the causal agents of the current Dutch elm disease pandemic in Europe and North America. The complete mitochondrial genome (mtDNA) of Ophiostoma novo-ulmi subsp. novo-ulmi, the European component of O. novo-ulmi, has been sequenced and annotated. Gene order (synteny) among the currently available members of the Ophiostomatales was examined and appears to be conserved and mtDNA size variability among the Ophiostomatales in part is due the presence of introns and their encoded open reading frames. Phylogenetic analysis of concatenated mitochondrial protein-coding genes yielded phylogenetic estimates for various members of the Ophiostomatales with strong statistical support showing that mtDNA analysis may provide valuable insights into the evolution of the Ophiostomatales.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
The mitogenome of Urnula craterium
No full textUrnula craterium (Schwein.) Fr. (1851) has been reported from North America, Europe, and Asia, and can be a pathogen on various hardwood species. In this study we investigated the mitochondrial genome of U. craterium. The biology and taxonomy of this fungus is poorly studied and there are no mitogenomes currently available for any member of the Sarcosomataceae (Order Pezizales). The complete mitogenome of U. craterium comprises 43 967 bps and encodes 14 protein-coding genes, a complete set of tRNAs and rRNA genes. A novel feature of the mitogenome is the presence of a single subunit DNA polymerase coding region that is typically associated with linear invertron-type plasmids. The mitogenome may offer insights into the evolution of mitogenomes among members of the Pezizales with regards to gene content and order, mobile elements, and genome sizes.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
