11,542,856 research outputs found
The people behind the papers - Wael Noor El-Nachef and Marianne Bronner
No full textThe enteric nervous system (ENS) derives from the neural crest and innervates the gastrointestinal system, in which it is essential for gut function throughout life. A new paper in Development uses zebrafish to investigate the poorly understood process of post-embryonic ENS neurogenesis, in both development and injury contexts. To find out more, we met the paper's two authors, Wael Noor El-Nachef, Assistant Clinical Professor of Medicine at UCLA, and Marianne Bronner, Albert Billings Ruddock Professor of Biology and Biological Engineering at Caltech
Endophragmiella bogoriensis Rifai, spec. nov. (Hyphomycetes)
No full textRIFAI, M.A. 2008. Endophragmiella bogoriensis Rifai, spec. nov. (Hyphomycetes). Reinwardtia 12 (4): 275 – 276. –– A new species of Endophragmiella is described and illustrated based on a specimen found growing on dead branchlets of Morinda citrifolia in Bogor, West Java, and compared with its closely related congeners thus far known
KOSTERMANSINDA RIFAI GENUS NOVUM HYPHOMYCETARUM
No full textThe conidial development of Sclerographium magnum Boedijn is describedand illustrated; based on this species the new aleuriosporous genus Kostermansinda Rifai is proposed
Abstract 1095: A combination of suberoylanilide hydroxamic acid and quinacrine is an effective therapeutic approach in preclinical settings of upper gastrointestinal cancers
No full textAbstract
Background: Quinacrine (QC), an antimalarial drug, has been shown to possess anticancer effects. Suberoylanilide hydroxamic acid (SAHA) inhibits class I and class II HDACs and is approved for cancer therapy. Developing novel approaches to overcome cancer drug resistance could significantly enhance current therapeutic approaches and improve patient care.
Methods: ATP-GLO, clonogenic survival, Annexin-V apoptosis assay, comet assay and DNA double-strand breaks (DSB) kits were used. The mRNA and protein levels were evaluated by quantitative real-time PCR and Western blot analyses.
Results: A combination of QC/SAHA significantly increased cell death in all cancer cell lines and had no effect on immortalized non-cancer cell lines (HFE145, NIH-3T3 and EPC2) (P<0.01). Clonogenic survival assay indicated that QC/SAHA co-treatment led to significantly lower number of cancer cell colonies, as compared to single agents and controls (P<0.01). Of note, the QC/SAHA combination led to an increase in the the sub-G0 population in AGS (9-fold), MKN-28 (14-fold), FLO1 (5.6-fold), and SNU1 (4-fold) cells (P<0.01). These results were confirmed using the Annexin V apoptosis induced significantly higher levels of apoptosis (10 - 20 fold) as compared to single agent and control (P<0.01). Treatment with QC/SAHA combination induced high levels of DSB (>20 fold, P<0.01). Comet assay data showed increased DNA damage compared with vehicle-treated cells (8-fold, P<0.01). Western blot analysis demonstrated a notable increase in activation of PARP, caspases 3, 9 and γ-H2AX following QC/SAHA co-treatment in all cancer cell lines. Interestingly, the combination of QC/SAHA substantially decreased the protein levels of both wtP53 and mutP53 in these cells. Tumor xenograft data confirmed that a combination of QC/SAHA is more effective than a single agent in abrogating tumor growth (P<0.05).
Conclusion: Our novel findings show that QC and SAHA have a synergistic effect on cancer cell death. The results provide compelling evidence that increased DNA damage mediates the cytotoxic effect of combined QC/SAHA. Such effect is likely related to mutP53 and wtP53 protein degradation induced by QC/SAHA combination. Our findings provide a rationale for a clinical evaluation of combined QC/SAHA therapy in gastro-esophageal cancers. Ongoing studies are currently being undertaken to understand mechanisms that regulate the degradation of wtp53 and mutp53 proteins.
Citation Format: Shoumin Zhu, Wael El-Rifai. A combination of suberoylanilide hydroxamic acid and quinacrine is an effective therapeutic approach in preclinical settings of upper gastrointestinal cancers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 1095. doi:10.1158/1538-7445.AM2017-1095</jats:p
Programmed chemotherapy for patients with metastatic unresectable gastric cancer.
No full textBACKGROUND: Recent advances in the treatment of metastatic unresectable gastric cancers (MGC) include the development of new antitumor drugs and new regimens for their use. However, the selection of individually designed regimens by gastric cancer (GC) subtype remains problematic. Here, we investigated the clinical usefulness of programmed chemotherapy. METHODOLOGY/PRINCIPAL FINDINGS: MGC patients were classified into three groups by clinical condition. We implemented a chemotherapy program consisting of S-1 combination regimens. Median survival time (MST) of level 1 patients was 416 days (95% CI: 313-506 days), with an overall response rate of 47%. MSTs of level 2 and 3 patients were 208 (95% CI: 153-287 days) and 95 days (95% CI: 28-136 days), respectively. Grade 3-4 toxicities were neutropenia in 12% and anorexia in 6%. All treatment- related toxicities were resolved, and no treatment-related deaths occurred. CONCLUSIONS/SIGNIFICANCE: This program provided reasonable selection of case-matching regimens and may improve the survival of patients with MGC. Further, it may represent the first clinical tool to provide efficient chemotherapy course selection for MGC. Ongoing analysis of newly developed drugs and regimens will allow the efficacy of this chemotherapy program to be improved
Unraveling the Regimes of Interfacial Thermal Conductance at a Solid/Liquid Interface
No full textThe interfacial thermal conductance at a solid/liquid interface (G) exhibits an exponential-to-linear cross-over with increasing solid/liquid interaction strength, previously attributed to the relative strength of solid/liquid to liquid/liquid interactions. Instead, using a simple Lennard-Jones setup, our molecular simulations reveal that this cross-over occurs due to the onset of solidification in the interfacial liquid at high solid/liquid interaction strengths. This solidification subsequently influences interfacial energy transport, leading to the cross-over in G. We use the overlap between the spectrally decomposed heat fluxes of the interfacial solid and liquid to pinpoint when “solid-like energy transport” within the interfacial liquid emerges. We also propose a novel decomposition of G into: i) the conductance right at the solid/liquid interface and ii) the conductance of the nanoscale interfacial liquid region. We demonstrate that the rise of solid-like energy transport within the interfacial liquid influences the relative magnitude of these conductances, which in turn dictates when the cross-over occurs. Our results can aid engineers in optimizing G at realistic interfaces, critical to designing effective cooling solutions for electronics among other applications. This data set contains the MD simulation files and post-processing codes to reproduce our results. The dataset is related to the publication by Abdullah El-Rifai, Sree Hari Perumanath Dharmapalan, Matthew K. Borg and Rohit Pillai (2024), "Unraveling the Regimes of Interfacial Thermal Conductance at a Solid/Liquid Interface", Journal of Physical Chemistry C, https://doi.org/10.1021/acs.jpcc.4c00536File structure described in README.tx
sw-rifai/eastern-Australia-CO2-NDVI-change: revision_1
No full textText and analysis code for Rifai et al., (202x) Biogeosciences
CAIRO. - The Mosques of Sultan Hassan and El-Rifai
No full textfront: "CAIRO. - The Mosques of Sultan Hassan and El-Rifai" ; back: "L. C. 448 : LE CAIRE. - Les Mosqueés de Sultan Hassan et El-Rifai". Postcard albumBuilding with three domes, four towers Men on path leading up to Mosqu
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