18,667 research outputs found
Development of 3D Cell Printed Patch-Type Stem Cell Therapy for Treatment of Liver Cirrhosis
No full textLiver cirrhosis is an irreversible end-stage liver disease which has no effective therapy except for liver transplantation, but donor shortage has been a critical limitation. Stem cell injection has been regarded as an alternative to the liver transplantation; yet, there is no optimal condition for such therapy. Moreover, the efficiency of delivery is below 10%, and the efficacy is reported to be minimal or even none. Therefore, we developed a liver patch, which is a 3D cell printed patch-type stem cell therapy with liver decellularized extracellular matrix (LdECM) bioink and human bone marrow derived mesenchymal stem cell to enhance the efficacy and delivery efficiency. The liver patch showed in vitro efficacy in the inactivation of the activated hepatic stellate cell (aHSC) by paracrine effect in transwell coculture condition. For in vivo efficacy assessment, the liver patch was implanted to the pathological liver tissue of irreversible cirrhosis mouse models. The deposited endogenous collagen was significantly decreased, and aHSCs were inactivated in the liver patch delivered cirrhosis model. Consequently, the results demonstrated that the liver patch with functional LdECM bioink and stem cell would be an effective therapy for liver cirrhosis treatment.1
3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications
No full textThe development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.11Ysciescopu
Extracellular matrix-based sticky sealants for scar-free corneal tissue reconstruction
No full text© 2022 The AuthorsRegenerative medicine requires both tissue restoration and ease of compliance for clinical application. Considering this, sticky tissue sealants have been shown to have great potentials over surgical suturing and wound treatment. However, tissue sealants currently used pose challenges such as uncontrollable adhesion formation, mechanical mismatch, and lack of tissue restoration. A new sticky sealant based on gelatinized cornea-derived extracellular matrix (GelCodE) with a visible light-activating system is firstly being introduced in this study. De novo tissue regeneration relies on the matrisome in charge of tissue-organization and development within GelCodE while visible light-based photopolymerization with ruthenium/sodium persulfate rapidly induces covalent bonds with the adjacent tissues. The ease of not only in vivo application, biocompatibility, and biointegration, but also exceptional de novo tissue formation is demonstrated in this study. Interestingly, newly regenerated tissues were shown to have normal tissue-like matrices with little scar formation. Hence, this work presents a promising strategy to meet clinical demands for scar-free tissue recovery with superior ease of clinical application.11Nsciescopu
Three-dimensional cell-printing of advanced renal tubular tissue analogue
No full textDespite significant progress in the development of renal tissue, recapitulation of perfusable complex renal tubular tissue with clinically relevant cellular heterogeneity is still remaining a challenge. In this study, using coaxial 3D cell-printing technique, we present microfluidic hollow tubes to realize tubular/vascular renal parenchyma composed of renal tubular epithelial and endothelial cells, respectively. We developed a functional hybrid bioink that inherits microenvironments for vascularized native kidney tissue with rapidly crosslinkable character to optimize cell functionality and retain the predefined hollow tubular structure. In addition, the novel bioink and 3D coaxial cell-printing technique provided a complex tube with tunable feature of monolayer and bilayer structure across the length of printed tube. Through prototyping a vascularized renal proximal tubule-on-a-chip, we showed its applicability to novel microfluidic renal tissue models. The renal subcapsular transplantation of the hollow tubes showed a long-term graft survival with the therapeutic capability of the tubular constructs in in vivo model of renal disease, which serves their applicability in regenerative medicine.11Nsciescopu
3D Bioprinting of Macro Hepatic Tissue Module Using Light-Activated Liver Decellularized Extracellular Matrix-Based Bioink
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3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering
No full text3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rely on homogeneous biomaterials, which has several shortcomings such as insufficient mechanical properties and recapitulation of microenvironment. In this study, we investigated the capability of skin-derived extracellular matrix (S-dECM) bioink for 3D cell printing-based skin tissue engineering. S-dECM was for the first time formulated as a printable material and retained the major ECM compositions of skin as well as favorable growth factors and cytokines. This bioink was used to print a full thickness 3D human skin model. The matured 3D cell-printed skin tissue using S-dECM bioink was stabilized with minimal shrinkage, whereas the collagen-based skin tissue was significantly contracted during in vitro tissue culture. This physical stabilization and the tissue-specific microenvironment from our bioink improved epidermal organization, dermal ECM secretion, and barrier function. We further used this bioink to print 3D prevascularized skin patch able to promote in vivo wound healing. In vivo results revealed that endothelial progenitor cells (EPC5)-laden 3D-printed skin patch together with adipose-derived stem cells (ASCs) accelerates wound closure, re-epithelization, and neovascularization as well as blood flow. We envision that the results of this paper can provide an insightful step towards the next generation source for bioink manufacturing. (C) 2018 Elsevier Ltd. All rights reserved.11Nsciescopu
Development of Mesenchymal Stem Cell Laden Patch-Type Therapy using 3D Cell-Printing Technology for Liver Cirrhosis Treatment
No full textLiver cirrhosis is an irreversible end-stage liver disease which has no effective therapy except for liver transplantation, but donor
shortage has been a critical limitation. Stem cell injection has been regarded as an alternative therapy to the liver transplantation; yet,
there is no optimal condition for such therapy. Moreover, the efficiency of delivery is below 10%, and the efficacy is reported to be
minimal or even none. Therefore, we developed a new stem cell delivery method, which is a 3D cell printed patch-type stem cell
therapy (liver patch) with liver decellularized extracellular matrix (LdECM) bioink and human bone marrow derived mesenchymal
stem cell to enhance the efficacy and delivery efficiency. The liver patch showed in vitro efficacy in the inactivation of the activated
hepatic stellate cell (aHSC) by paracrine effect in transwell coculture condition. For in vivo efficacy assessment, the liver patch was
implanted to the pathological liver tissue of irreversible cirrhosis mouse models. The deposited endogenous collagen was significantly
decreased, and aHSCs were inactivated in the liver patch delivered cirrhosis model. Consequently, the results demonstrated that the
liver patch with functional LdECM bioink and stem cell would be an effective therapy for liver cirrhosis treatment.2
3D Cell-printing of prevascularized stem cell patch for liver cirrhosis treatment
No full textLiver cirrhosis is an irreversible liver failure which has no curative therapy except liver transplantation, but it is limited by a donor shortage. Stem cell transplantation has been regarded as an alternative therapy to the liver transplantation; yet, the delivery efficiency of stem cells and the efficacy are still controversy. Therefore, we developed a novel stem cell therapy, which is a hBMMSC (human bone marrow derived mesenchymal stem cell) / EPC (endothelial progenitor cell)-laden prevascularized stem cell patch using 3D cell-printing technology with liver decellularized extracellular matrix (LdECM) bioink to enhance the efficacy and delivery efficiency of stem cell therapy. In the in vitro study, the prevascularized stem cell patch showed anti-fibrotic effect on the activated hepatic stellate cell (aHSC) by paracrine effect. For in vivo efficacy assessment, the stem cell patch was transplanted to the pathological liver tissue of irreversible cirrhosis mouse models for 4 weeks. In the stem cell patch transplanted cirrhosis model, the hBMMSCs remained much more than the stem cell injected group during implantation, and the deposited endogenous collagen was significantly decreased. Moreover, fibrosis markers were significantly decreased in the stem cell patch delivered cirrhosis model. Consequently, the results demonstrated that the stem cell patch with functional LdECM bioink and stem cell would be an effective therapy for liver cirrhosis treatment.1
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