1,074 research outputs found
Fluorescence in situ hybridization (FISH) for genomic investigations in rat.
No full textThis chapter concentrates on the use of fluorescence in situ hybridization (FISH) for genomic investigations in the laboratory rat (Rattus norvegicus). The selection of protocols included in the chapter has been inspired by a comprehensive range of previously published molecular cytogenetic studies on this model organism, reporting examples of how FISH can be applied for diverse investigative purposes, varying from comparative gene mapping to studies of chromosome structure and genome evolution, to characterization of chromosomes aberrations as well as transgenic insertions. The protocols, which include techniques for the preparation of mitotic chromosomes and DNA fibers from short-term cell cultures, have been gathered through the years and repeatedly tested in our laboratory, and all together aim at providing sufficient experimental versatility to cover a broad range of cytogenetic and cytogenomic applications
Bildungswerk BLITZ e.V. - the multicultural centre of education, job opportunities and entertainment
No full textDie Abschlussarbeit befasst sich in ihrem theoretischen Teil mit der Gesamtkonzeption und den Zielen des deutschen Bildungswerkes BLITZ e.V. Dieses wird insbesondere durch die Beschreibung seiner Entstehung, Entwicklung und der wichtigsten Projekten vorgestellt. Weiterhin soll auf den Förderverein BLITZ e.V., und dessen unterstützende Hilfe eingegangen werden. Das darauffolgende Kapitel soll das europäische Projekt „Freiwilliger Dienst“ beschreiben. Der zweite Teil dieser Arbeit behandelt den Praxiseinsatz des Autors beim Bildungswerk BLITZ e.V. im Zuge der Veranstaltung -„Kinderdorf 2009“.Bakalářská práce se ve své teoretické části zabývá celkovou koncepcí a cíli německého "Vzdělávacího střediska BLITZ e.V.". To je představeno především popisem jeho vzniku, vývoje a jednotlivých projektů. Zmíněn je také Förderverein BLITZ e.V. a jedna z kapitol je taktéž věnována evropskému projektu "Evropská dobrovolná služba". Druhá část pojednává o autorově praxi ve vzdělávacím středisku BLITZ e.V., v dětské vesničce "Kinderdorf 2009". Ta je v závěrečné práci důkladně popsána a doplněna o vlastní zhodnocení.In its theoretical part, the Bachelor thesis deals with the overall concept and aims of the German "Educational centre BLITZ e.V.". It is introduced particularly through a chronological description of its formation, development and individual projects. Also Förderverein BLITZ e.V. is mentioned and one chapter is also dedicated to the "European voluntary service". The second part deals with author´s training in BLITZ e.V., in the children´s village, "Kinderdorf 2009". The training is described in detail in the Bachelor thesis and is completed with author´s assessment.Katedra cizích jazykůDokončená práce s úspěšnou obhajobo
A Remote Area Power Supply (RAPS) Training Facility utilising SMA Sunny Island Inverter technology and National Instruments measurement package
No full textRAPS systems can sometimes be a cheaper alternative for supplying power than connection to a distant utility. Most assuredly it is a cleaner method of producing electricity than coal or gas constituents, provided the diesel generator is used minimally. A modern RAPS system will generally have several sources of generation, typically they might be; solar, wind and a diesel/petrol generator coupled with a battery to store excess power. The RAPS 2 system at Murdoch University was selected for an overhaul early in 2010. The system was redesigned to include new inverter technology from SMA. The new system relies on 3 separate inverters to convert the sources directly into grid quality electricity, as opposed to some traditional RAPS systems that would have most of its elements feed a DC bus that connected to a single inverter. This novel way of setting up a mini grid finds its niche in situations when the terrain or meteorological conditions make it impractical to have generation equipment all localised and a DC bus would have higher associated cable losses.
For such a system to be of educational benefit to tertiary students, the variables within the system must be able to be monitored. To this end an advanced measurement system has been designed and procured so that every measurable point in the upgraded system is available for display.
The measurement system was designed using National Instruments compact DAQ (data acquisition) hardware, one of the first of its type to be introduced to the university. The compact DAQ unit has been coupled with isolation equipment supplied by Dataforth, which already has an existing prevalence throughout the engineering department. The subsequent measurement program monitors 31 system variables at a high sampling rate to provide real time information on the state of the system. The measurement program also monitors relevant meteorological aspects such as the solar irradiance and local wind speed.
This report documents and details the steps taken to set up a new and novel RAPS system. Discussed are the approaches taken throughout the semester to project manage and facilitate progress. A detailed description is also given of the measurement and protection system that has been designed to allow students and the general public to get a good grasp on the performance of the system
Compendio delle principali eruzioni vesuviane dall'anno 79 e.v. /
No full textIncludes bibliographical references.Mode of access: Internet.Library's copy is signed by author
Internal investigations under German Law
No full textFolker Bittmann is a chief prosecutor and has served as head of the Public Prosecutor’s Office in Dessau-Roßlau, Germany since 2005. Prior to his current position, he worked as a lawyer in Heidelberg, a prosecutor in Darmstadt and Frankfurt, and a chief prosecutor in Halle/Saale. He is the editor and author of a handbook on criminal insolvency law, as well as an author of various articles on property law, white-collar crime, and criminal procedure law. Additionally, he is an advisor to an association on white-collar crime (Wirtschaftsstrafrechtliche Vereinigung e.V.) and is a member of the practice group of the Center for Criminal Compliance at the University of Gießen
LIGHT-DARK CYCLE AND MITOTIC INDEX IN ASELLUS-AQUATICUS (L) (CRUSTACEA, ISOPODA)
No full textThe circadian variation of the mitotic index during spermiohistogenesis was studied in Asellus aquaticus (L.). The actual number of metaphases and prometaphases was determined at the end of each hour of light or darkness over a 24 h period in animals bred under LD 12:12. The number of the metaphases and prometaphases decreases during the light period and sharply increases in the last 3 hrs of the dark period. This variation in the proliferative activity suggests that photoperiod can play a role in the synchronization of mitosis
WebWriter: A System to Author and Remix Explorables – Requirements & First Prototype
No full textActive and personalized learning requires that teachers can remix a wide variety of sources into new learning resources, which in turn may be customized by students. While this is well-supported for paper-oriented resources, multimodal, interactive learning resources for digital use, called explorables, are much more difficult to author and remix. In this paper, we derive a set of requirements for a system to author such explorables from a workshop-based study. We further present a first prototype of such a system, called “WebWriter”
Un anello ebraico in bronzo da Porto Torres (sec. IV-V e.v.), e l'epigrafe di Anabatia rinvenuta a Gerace (sec. XIV), con una nota su una lucerna funeraria ebraica da Licata (secc. IV-V e.v.)
No full textThe study presents a number of new archaeological discoveries of Jewish artifacts and proposes a new reading of an already known inscription. Perani, author of Part I, describes a recently discovered Jewish bronze ring from Porto Torres, Sardinia, dating to late antiquity (4th-5th centuries e.v.), relating to similar Jewish rings, discovered in Sicily and Sardinia in the past. Colafemmina, author of Part II, presents his reading of the inscription of Anabatyya found in 1990 in Gerace, province of Reggio Calabria. Finally, M.S. Rizzo and A. Toscano Raffa describe a recently discovered Jewish funerary lamp with menorah from late Roman period (4th-5th centuries), just found in Licata (Sicily)
PO-018 Acquired DNA damage in adolescent obesity – a promoter and predictor of cancer?
No full textIntroduction
Epidemiological evidence linking obesity with increased risk of cancer is steadily growing, although the causative aspects underpinning this association are only partially understood. Obesity coincides with deficiencies in micronutrients such as Vitamin D, a key player in DNA repair processes. As a result, vitamin D deficiency in obesity may have a marked impact on DNA stability and integrity. 8-hydroxyguanosine (8-OHdG) is a well-established marker of oxidative DNA damage that has been identified in higher concentrations in cancer patients. Here we report, preliminary, unpublished findings from our study on acquired DNA damage in childhood obesity.
Material and methods
Body Mass Index (BMI), Waist to Hip ratio (WHR) and body fat percentage via bioelectrical impedance was assessed in over 70 participants, aged 11–18 and recruited from National Health Service (NHS) obesity clinics and schools in London. A non-invasive, integrated evaluation of urinary 8-OHdG and salivary vitamin D was conducted using ELISA based methods and compared to markers of adiposity.
Results and discussions
A BMI percentile >99 was found to be associated with decreased salivary vitamin D and increased urinary 8-OHdG when compared to healthy weight controls (BMI=5th-85th percentile). Vitamin D levels in saliva were found to be inversely correlated with BMI and body fat percentage. Urinary 8-OHdG positively correlated with body fat percentage and WHR. Most importantly, an inverse correlation between vitamin D in saliva and 8-OHdG in urine was also identified.
Recent evidence has suggested vitamin D in obesity to be a consequence of altered behaviour, reduced intestinal absorption, and sequestration of vitamin D into adipose tissue. As a result, DNA repair processes against oxidative DNA damage in obesity may be impaired, resulting in the excess of lesions including 8-OHdG. The effects of excess 8-OHdG lesions have been well researched to include various mutations that can drive carcinogenesis.
Conclusion
Our results suggest Vitamin D deficiency in obese adolescents may play a significant role in triggering oxidative DNA damage, thus increasing the likelihood of cancer later in life
Controversies in the management of acute tonsillitis: an evidence-based review
No full textBACKGROUND: Patients admitted with acute tonsillitis generate a substantial workload for the National Health Service (NHS), placing huge financial pressures on an already overstretched budget.OBJECTIVE OF REVIEW: Due to the difficulty of accurate diagnosis and varying practices across the UK, there exist a number of controversial areas and non-standardised practice. These will be highlighted and tackled within this article.TYPE OF REVIEW AND SEARCH STRATEGY: A literature review, last performed in September 2013, searched PubMed citing variations on the areas of controversies with 'tonsillitis', 'pharyngitis' and 'pharyngotonsillitis' - from 1956 to present with language restrictions. Excluded articles included those regarding sore throat after tonsillectomy and peritonsillar abscess.EVALUATION METHOD: Titles and abstracts were initially screened, and full text of potentially relevant articles obtained. The bibliographies of articles were searched for relevant references. The references were then compiled and reviewed independently by two authors (JB & TB), overseen by the senior author (EK).RESULTS: (i) Diagnosis and investigation: Use of the Centor criteria is inadequate within the secondary care setting. Blood testing is unnecessary in the majority of cases where patients do not require admission, as they are unlikely to change management. (ii) Antibiotics: Antibiotics are likely to be indicated in all those presenting to secondary ENT care, with penicillin being the antibiotic of choice for first-line therapy. (iii) Corticosteroids: Moderate evidence supports the benefit of steroid administration in this patient cohort, advocating a single dose initially followed by reassessment. (iv) Analgesia: Paracetamol and NSAIDs have good evidence of action. Codeine should be used with caution in the paediatric population. (v) Reduced admission rates and early discharge: There is evidence suggesting that a trial of medical therapy prior to admission is beneficial in reducing rates of admission and length of stay.CONCLUSIONS: Management of acute tonsillitis within a secondary care setting largely consists of anecdotal or relatively low-quality evidence. Thus, much evidence from management comes from expert opinion or practice within a primary care setting. Management across the UK can also vary greatly. An evidence-based review of best practice has been presented here, but further evidence will be required in the future examining the significance of corticosteroids and antibiotic administration in this patient cohort specifically, ensuring practice is evidence based and clinically relevant.</p
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