4 research outputs found
Pituitary corticotropin-inhibiting peptide: properties and use in study of corticotropin action
No full textThe biological properties of the naturally occurring pituitary peptide αh7−38-adrenocorticotropin (ACTH) have been investigated. αh7−38-ACTH is devoid of steroidogenic activity but inhibits competitively ACTH-induced steroidogenesis in vitro as well as in vivo. The long-term actions of ACTH on normal and tumor adrenal cells in culture are also antagonized by αh7−38-ACTH. The apparent Ki for the inhibition of cyclic AMP production by αh7−38-ACTH (301±62 nM) was significantly higher than the apparent Ki for the inhibition of corticosterone synthesis (21.6±6.8 nM). Analysis of the inhibition of ACTH-induced steroidogenesis and cyclic AMP production in normal rat adrenocortical cells indicates that two separate receptors may be involved in mediating these responses
Developmental Difficulties and Developmental Priorities of Communes and Towns of Ličko-senjska County in Local Experts Optics
No full textThe paper is founded upon the survey research in developmental aspirations and attitudes
made in 1996 for the purpose of making the programme of spatial regulation of Licko-senjska
County, which is undeveloped and impoverished rural area. The research was carried
out by means of inquiry made among the representatives of local expert groups rom
seven towns and communes of the County. Because of the breach in systematically carried
out researches, this paper is among the rare empirical scientific works on undevelope
rural areas in Croatia made in the last ten years. The insight in developmental resources
and factors and in development planning and organization is not possible without socia
and above all, sociological researches. The price of this situation is known: for pure or no
insight at all, developmental resources are being wasted and many actions aiming at revitalization
of economically and demographically depressed regions have failed.
The results of the research of perception of the way and extent the towns’ and communes’
belong to the County, of the judgement of developmental difficulties and priorities set by
towns and communes as well as suggestions given by the participants of inquiry, are given
in this paper by towns and communes. The author concludes with deduction and interpretation
of common characteristics. First, because of the size and heterogeneity of the
sparsely populated region and in the absence of a strong centre as well as because of the
influence of Rijeka, Zagreb and Split macroregions, the cohesion of Licko-senjska County
is very poor and the question of its identity is still open. Second, this County undoubtedly
belongs to Croatian periphery with prevailing rural communities in all its communes and
in small undeveloped towns without motivation for setting development in motion.
Common causes generating County’s developmental problems and originating particu ar
developmental difficulties and priorities make the contents of the characteristics mentione
The Hepatocellular Transport of Sulfobromophthalein-Glutathione by Clofibrate Treated, Perfused Rat-Liver
No full textThe hypolipidemic drug clofibrate is known to affect the hepatic transport of various organic anions including bilirubin, fatty acids and sulfobromophthalein. Changes in the rate of metabolism and/or intracellular transport have been claimed responsible for the effect. To evaluate these possibilities, the transport of sulfobromophthalein-glutathione, a model compound that does not require metabolism for biliary excretion, was studied in perfused livers isolated from clofibrate-treated and control rats. Cytosolic fatty acid binding protein and glutathione S-transferase activity were also measured. Clofibrate treatment significantly increased liver weight; as a result glutathione S-transferase activity (toward 1-chloro-2,4-dinitrobenzene) fell if expressed per gram of liver (4560±420 (SE) vs 7010±260 nmoles/min for clofibrate treated and controls respectively, p0.5). Irrespective of how it was expressed fatty acid binding protein was significantly increased by the drug treatment. Steady state sulfobromophthalein-glutathione removal velocity was saturble with increasing concentrations of sulfobromophthalein-glutathione in both control and clofibrate-treated livers. Steady state extraction ratio, as well as Vmax and Km for removal, did not differ between the two groups. In keeping with other observations, these data collectively indicate that the hepatic steady state removal of nonmetabolized compounds is not affected by clofibrate. Because the concomitant decrease in glutathione S-transferase activity only reflects an opposite change in liver weight, it remains to be determined whether clofibrate alters the hepatic transport of sulfobromophthalein and other compounds that are conjugated with glutathione solely by changing their rate of metabolis
